Nitrogen partitioning in oak leaves depends on species, provenance, climate conditions and soil type

Hu, Baishi; Simon, Judy; Kuster, Thomas; Arend, Matthias; Siegwolf, Rolf; Rennenberg, Heinz

doi:10.1111/j.1438-8677.2012.00658.x

Cited by 30 publications

(23 citation statements)

References 56 publications

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“…The extraction of amino acids from plant material was conducted after the method of Winter et al [58] and Hu et al [59] with slight modifications. Approximately 50 mg of frozen leaf or 80 mg root powder were mixed with 1 mL methanol/chloroform (Merck KGaA, Darmstadt, Germany) (3.5/1.5, v/v) and 0.2 mL Hepes buffer (containing 20 mM Hepes (Merck KGaA, Darmstadt, Germany), 5 mM EGTA (Sigma-Aldrich Inc., St. Louis, MO, USA), 10 mM NaF (Sigma-Aldrich Inc., St. Louis, MO, USA)).…”

Section: Protein and Amino Acid Analysesmentioning

confidence: 99%

Nitrogen Nutrition of European Beech Is Maintained at Sufficient Water Supply in Mixed Beech-Fir Stands

Magh

Yang

Rehschuh

et al. 2018

Forests

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Research highlights: Interaction effects of coniferous on deciduous species have been investigated before the background of climate change. Background and objectives: The cultivation of European beech (Fagus sylvatica L.) in mixed stands has currently received attention, since the future performance of beech in mid-European forest monocultures in a changing climate is under debate. We investigated water relations and nitrogen (N) nutrition of beech in monocultures and mixed with silver-fir (Abies alba Mill.) in the Black Forest at different environmental conditions, and in the Croatian Velebit at the southern distribution limit of beech, over a seasonal course at sufficient water availability. Material and methods: Water relations were analyzed via δ13C signatures, as integrative measures of water supply assuming that photosynthesis processes were not impaired. N nutrition was characterized by N partitioning between soluble N fractions and structural N. Results: In the relatively wet year 2016, water relations of beech leaves, fir needles and roots differed by season, but generally not between beech monocultures and mixed cultivation. At all sites, previous and current year fir needles revealed significantly lower total N contents over the entire season than beech leaves. Fir fine roots exhibited higher or similar amounts of total N compared to needles. Correlation analysis revealed a strong relationship of leaf and root δ13C signatures with soil parameters at the mixed beech stands, but not at pure beech stands. While glutamine (Gln) uptake capacity of beech roots was strongly related to soil N in the monoculture beech stands, arginine (Arg) uptake capacities of beech roots were strongly related to soil N in mixed stands. Conclusions: Leaf N contents indicated a facilitative effect of silver-fir on beech on sites where soil total N concentrations where low, but an indication of competition effect where it was high. This improvement could be partially attributed to protein contents, but not to differences in uptake capacity of an individual N source. From these results it is concluded that despite similar performance of beech trees at the three field sites investigated, the association with silver-fir mediated interactive effects between species association, climate and soil parameters even at sufficient water supply.

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Section: Protein and Amino Acid Analysesmentioning

confidence: 99%

Nitrogen Nutrition of European Beech Is Maintained at Sufficient Water Supply in Mixed Beech-Fir Stands

Magh

Yang

Rehschuh

et al. 2018

Forests

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“…In many forest species, organic solutes, specifically soluble carbohydrates and free amino acids, are the principal compounds involved in osmotic adjustment (Patakas et al, 2002). At the molecular level, most of these responses depend on proteins, therefore comparison of the protein profile of tolerant and sensitive populations can provide valuable information to predict the performance of a given genotype under drought conditions (Hu et al, 2012). …”

Section: Introductionmentioning

confidence: 99%

Drought Tolerance in Pinus halepensis Seed Sources As Identified by Distinctive Physiological and Molecular Markers

et al. 2017

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Drought is one of the main constraints determining forest species growth, survival and productivity, and therefore one of the main limitations for reforestation or afforestation. The aim of this study is to characterize the drought response at the physiological and molecular level of different Pinus halepensis (common name Aleppo pine) seed sources, previously characterized in field trials as drought-sensitive or drought-tolerant. This approach aims to identify different traits capable of predicting the ability of formerly uncharacterized seedlings to cope with drought stress. Gas-exchange, water potential, photosynthetic pigments, soluble sugars, free amino acids, glutathione and proteomic analyses were carried out on control and drought-stressed seedlings in greenhouse conditions. Gas-exchange determinations were also assessed in field-planted seedlings in order to validate the greenhouse experimental conditions. Drought-tolerant seed sources presented higher values of photosynthetic rates, water use efficiency, photosynthetic pigments and soluble carbohydrates concentrations. We observed the same pattern of variation of photosynthesis rate and maximal efficiency of PSII in field. Interestingly drought-tolerant seed sources exhibited increased levels of glutathione, methionine and cysteine. The proteomic profile of drought tolerant seedlings identified two heat shock proteins and an enzyme related to methionine biosynthesis that were not present in drought sensitive seedlings, pointing to the synthesis of sulfur amino acids as a limiting factor for drought tolerance in Pinus halepensis. Our results established physiological and molecular traits useful as distinctive markers to predict drought tolerance in Pinus halepensis provenances that could be reliably used in reforestation programs in drought prone areas.

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“…For this process, the mixture underwent ultrasonic extraction for 1 h; the extract was centrifuged at 13,200 r/min for 10 min at 4 °C. The supernatant was measured by the HPLC-MS method (Hu et al 2013). Amino acid content was determined by BEH Amide, 1.7 mu M, 100 × 2.1 mm column (liquid phase model UltiMate 3000, Thermo Scientific™ production; mass spectrometry model is Q Exactive™ production).…”

Section: Plant Sampling and Analysismentioning

confidence: 99%

The effects of fall fertilization on the growth of Chinese pine and Prince Rupprecht’s larch seedlings

Zhu

Wang

et al. 2019

J. For. Res.

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acids were analyzed. Results showed that: (1) stem height, diameter and biomass for both species were similar between controls and fall fertilization treatments; (2) compared to controls, fall fertilization increased Chinese pine needle and root N by 17.7% and 36.9%, respectively. For Prince Rupprecht's larch, fall fertilization resulted in 26.3% and 34.54% more N in stem and roots, respectively, than controls; (3) the three main amino acids in control and fertilization treatments in Prince Rupprecht's larch seedlings were glutamine, arginine and proline, and in Chinese pine seedlings were glutamine, arginine and γ-amino butyric acid; (4) total amino acid contents were not significantly increased by fall fertilization, but glutamine in Chinese pine and Prince Rupprecht's larch increased by 64.2% and 35.2%, respectively. Aboveground biomass of Prince Rupprecht's larch had higher proline contents than Chinese pine, which suggests that the stress resistance of the aboveground tissue may be higher for Prince Rupprecht's larch. The results indicate that different plant organs with various response are well adapted to nitrogen loading for nutrient storage in evergreen and deciduous conifer seedlings.

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Nitrogen partitioning in oak leaves depends on species, provenance, climate conditions and soil type

Cited by 30 publications

References 56 publications

Nitrogen Nutrition of European Beech Is Maintained at Sufficient Water Supply in Mixed Beech-Fir Stands

Nitrogen Nutrition of European Beech Is Maintained at Sufficient Water Supply in Mixed Beech-Fir Stands

Drought Tolerance in Pinus halepensis Seed Sources As Identified by Distinctive Physiological and Molecular Markers

The effects of fall fertilization on the growth of Chinese pine and Prince Rupprecht’s larch seedlings

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