Nitrogen fixing efficiency and physiological characteristics of Azospirillum isolates from the paddy fields of North Bengal

Hossain, MK; Akter, Salina; Hasan, Mahmudul; Hasan, Ashraful; Uddin, Kazi Rasel; Parvin, Afroza; Jahan, Israt; Rahman, Nazibur; Rahman, SM Badier

doi:10.3329/jujbs.v3i1.28277

Cited by 3 publications

(4 citation statements)

References 3 publications

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“…(Table 2). Among other factors, the nitrogen fixing abilities of Azospirillum isolates depend on nitrogenase enzyme activity (Sant'Anna et al 2011). Hossain et al (2014 reported that nitrogen fixing efficiency of Azospirillum sp.…”

Section: Resultsmentioning

confidence: 99%

“…Azospirillum colonizes the root region of crop plants in large numbers and fixes substantial amount of nitrogen (Saikia et al 2013). Crop production response to bacteria with Azospirillum inoculants was almost equivalent to that of application of 15 -20 kg N/ha (Hossain et al 2014). Inoculation with Azospirillum brasilense increased grain production of maize (Morais et al 2016).…”

Section: Introductionmentioning

confidence: 92%

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Isolation and identification of Azospirillum and Azotobacter species from Acacia spp. at Riyadh, Saudi Arabia

et al. 2019

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Populations of Azospirillum and Azotobacter isolated and identified from the soil rhizosphere of Acacia ehernbergiana, A. gerrardii and A. tortilis from three sites varied widely irrespective of tree species, size and site. Azospirillum strains were purified and characterized through morphological, microscopic and biochemical tests for motility, cell shape, pigmentation, colony color, gram reaction, optimal temperatures, biotin requirement, pH growth, acidification of peptone glucose medium, ability of nitrogen fixation, growth on 3% NaCl, and D-glucose and D-ribose utilization. Twenty Azospirillum isolates were divided into four matching characters. They were identified as A. lipoferum, A. brasilense, A. dobereinerae and A. zeae. The same above tests with omission of temperatures, biotin requirement, and acidification of peptone and addition of starch hydrolysis, H2S, urease, oxidase and sucrose, maltose and mannitol as carbon source were applied to Azotobacter strains. Azotobacter isolates were divided into three matching characters. They were identified as A. chroococcum, A. beijerinckii and A. salinestris.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 92%

Isolation and identification of Azospirillum and Azotobacter species from Acacia spp. at Riyadh, Saudi Arabia

et al. 2019

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“…After completing the requisite incubation, the plates were flooded with iodine solution to test the isolates starch digestion capacity. A yellow zone around the colony indicated starch hydrolysis, while a blue/black area indicated starch presence (Hossain et al, 2015).…”

Section: Starch Hydrolysis Testmentioning

confidence: 99%

Influence of Foliar Spray of Bio-Inoculant and Micronutrient on Soil Biology and Yield in Broccoli

Chandra¹,

Dash²

2023

AJMBES

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The experiment was carried out in the years 2021-22, at the Department of Agricultural Microbiology, Indira Gandhi Krishi Vishwavidyalaya, Raipur (Chhattisgarh). The study involved application of foliar spray of bio-inoculants and micronutrient; Ammonium Molybdate. Bio-inoculants; Azotobacter and Azospirillum inoculation strains were collected from Microbiology repository and were revived for biochemical characteristics on Jensen’s and Okon’s media respectively. Both the isolates showed positive reaction for starch hydrolysis test, catalase test, urease test and oxidase test. Plant parameters evaluated include biomass accumulation and fruit weight per plant. The experimental results revealed that significantly maximum fruit weight per plant (359.0g), dry shoot biomass (16.6g/ plant), dry root biomass (8.6g/ plant) were recorded under T4 (IF + Foliar Application of Bio-inoculants and Ammonium molybdate 0.2%). Besides higher microbial population, Azotobacter (44.3 x 103 cfu/ g of soil), Azospirillum (28.5x 103 cfu/ g of soil) were also observed to be significant under T4. Dehydrogenase activity in rhizosphere soils was found maximum 27.3 µg TPF/g soil/h under T4 (IF + Foliar Application of Bio-inoculants and Ammonium molybdate 0.2%) at 60DAT. Thus application of Bio-inoculants along with Ammonium molybdate (0.2%) as foliar spray along with 50% inorganic fertilizer proved to be the most effective and may be adopted in Broccoli cultivation.

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“…The highest efficiencies of nitrogen fixation first reported by Dobereiner and Day, (1976) 115 mg N 2 fixed/ g lactate which has not been reported in other studies, Okon et al, (1977) reported values of 20 to 24 mg N 2 fixed/ g substrate, while Lakshmi et al, (1977) recorded from 12 to 36 mg N 2 fixed/ g substrate, Nelson and Knowles, (1978) reached to 28 mg N 2 fixed/ g substrate, as well as Khan et al, (2001) recorded that the N 2 -fixing potentials of Azospirillum isolated from wheat fields of Dhaka ranged from 15.12 : 22.16 mg N 2 fixed/g substrate, and Azospirillum isolated from Bangladesh was fixed nitrogen and ranged from 10.08 : 28.00 mg N 2 fixed/g substrate, Kanimozhi and Panneerselvam, (2010) ranged from 3.3 to 15.6 mg N 2 fixed/g substrate, while Hossain et al, (2014) ranging from 10.03 to 13.11 mg N 2 fixed/ g substrate (malate), finally Bharathiraja, (2019) ranged from 1.03: 42.30 mg N 2 fixed/g cell weight.…”

Section: Determination Of N 2 Fixationmentioning

confidence: 99%

Isolation and characterization of local Azospirillum and yeast strains to be used for wheat grains inoculation to improve plant growth and yield under field cultivation

Ahmed

Badawy

Mahmoud

et al. 2022

AJAS

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Four Azospirillum strains and three yeast isolates were isolated from rhizosphere soil of wheat plants grown on Soil of the Experimental Farm of Soil and Water Department, Assiut University, Egypt. There were studied the characteristic of both Azospirillum and yeast to be used as a biofertilizers in inoculation of wheat grains under field condition to study the response of growth and yield to inoculation. For Azospirillum, the isolation was on Nitrogen free bromothymol (Nfb) semisolid medium, their morphology (light microscope, scanning electron microscope), cultural characteristics to verity their identity to those reported on Bergey's Manual of Determinative Bacteriology (1994), and N2fixing capacities, which were ranged from 13.8:42.5 mg N2-fixed/g malic acid. For yeast, the isolation was on Malt Extract Peptone Glucose (MEPG) medium, their morphology, cultural characteristics and acid production were determined, as well as the promotive stimulating effects of Azospirillum and yeast on grain germination and early seedling growth of wheat.

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Nitrogen fixing efficiency and physiological characteristics of Azospirillum isolates from the paddy fields of North Bengal

Cited by 3 publications

References 3 publications

Isolation and identification of Azospirillum and Azotobacter species from Acacia spp. at Riyadh, Saudi Arabia

Isolation and identification of Azospirillum and Azotobacter species from Acacia spp. at Riyadh, Saudi Arabia

Influence of Foliar Spray of Bio-Inoculant and Micronutrient on Soil Biology and Yield in Broccoli

Isolation and characterization of local Azospirillum and yeast strains to be used for wheat grains inoculation to improve plant growth and yield under field cultivation

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