Nitrogen Fixation by the Green and Purple Sulfur Bacteria

Lindstrom, E. S.; Tove, Shirley; Wilson, P. W.

doi:10.1126/science.112.2903.197

Cited by 59 publications

(21 citation statements)

References 5 publications

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“…Nitrogen fixation by autotrophic green photosynthetic bacteria of the genus Chlorobium was first demonstrated by Lindstrom, Tove & Wilson (1950). We have now shown that the green heterotrophic photosynthetic bacterium Chloropseudomonas ethylicum can also grow with Nz as nitrogen source.…”

Section: (Accepted For Publication 17 November 1970)mentioning

confidence: 86%

Nitrogen Fixation by the Green Photosynthetic Bacterium Chloropseudomonas ethylicum

Evans

1971

Journal of General Microbiology

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Nitrogen fixation by autotrophic green photosynthetic bacteria of the genus Chlorobium was first demonstrated by Lindstrom, Tove & Wilson (1950). We have now shown that the green heterotrophic photosynthetic bacterium Chloropseudomonas ethylicum can also grow with Nz as nitrogen source. The mechanism of nitrogen fixation by green bacteria has not been investigated and extracts with nitrogenase activity have not previously been prepared from this group of bacteria.Chloropseudomonas ethylicum strain N 2, obtained from Dr N. Kondratieva, was grown in a modified Pfennig's medium with ethanol as substrate as described previously (Evans, I 968). For growth under nitrogen-fixing conditions, ammonium chloride was omitted and the medium gassed with 95 % nitrogen, 5 % carbon dioxide. Chloropseudomonas ethylicum has been subcultured continuously for up to 3 months under these conditions. Growth was initiated by employing a 5 % inoculum of a N, grown culture, and after 48 h. growth the organisms were harvested with a continuous flow centrifuge. The paste was stored frozen until required.Frozen paste was suspended I : 2 (w/v) in 0.02 M-tris-HCl (pH 7-3). The buffer was deoxygenated by bubbling with N, before use. The suspension was cooled in ice under argon for 5 min. and exposed to sonic oscillation under an atmosphere of argon or nitrogen using a Dawe Soniprobe on setting 8 for omi in. The broken suspension was centrifuged for 30 min. at 150,000g av. and the clear brown supernatant used as the crude extract without further treatment.In some experiments oxygen-free 0.02 M-tris-HCl pH 7.3 containing 0.25 M-NaCl was used. Ferredoxin was removed by passing these extracts through an anaerobic 2 x 1-5 cm. column of DEAE cellulose equilibrated with the same buffer. Two ml. of an 0

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Section: (Accepted For Publication 17 November 1970)mentioning

confidence: 86%

Nitrogen Fixation by the Green Photosynthetic Bacterium Chloropseudomonas ethylicum

Evans

1971

Journal of General Microbiology

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“…rubrum was capable of nitrogen fixation (Kamen and Gest, 1949). Confirmation of this was made by measurement of the fixation of into cells of Rs, rubrum and other species of purple bacteria (Lindstrom et al, 1949), and several papers appearing shortly afterwards suggested that the capacity to fix nitrogen was widely distributed among purple and green bacteria (Lindstrom et al, 1950(Lindstrom et al, ,1951Newton and Wilson, 1953). Abbreviations: Cf.…”

Section: A Discovery Of Fixation In Anoxygenic Photosynthetic Bacteriamentioning

confidence: 95%

Microbiology of Nitrogen Fixation by Anoxygenic Photosynthetic Bacteria

Madigan

Advances in Photosynthesis and Respiration

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“…This hypothesis was validated by 15N incorporation experiments. Lindstrom and co-workers quickly applied the 15N2 technique to other representatives of the known photosynthetic bacterial groups including Rhodopseudomonas, Chromatium, Chlorobium, and Rhodomicrobium, and showed that the ability was common among the photosynthetic bacteria (Lindstrom et al, 1949(Lindstrom et al, , 1950(Lindstrom et al, , 1951.…”

Section: Discovery Of New N2-fixing Organismsmentioning

confidence: 96%

Consumption of Atmospheric Nitrogen

Benson

1985

Bacteria in Nature

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Nitrogen Fixation by the Green and Purple Sulfur Bacteria

Cited by 59 publications

References 5 publications

Nitrogen Fixation by the Green Photosynthetic Bacterium Chloropseudomonas ethylicum

Nitrogen Fixation by the Green Photosynthetic Bacterium Chloropseudomonas ethylicum

Microbiology of Nitrogen Fixation by Anoxygenic Photosynthetic Bacteria

Consumption of Atmospheric Nitrogen

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