Nitrogen Fixation by a Facultative Bacillus

Hino, Seiichi; Wilson, P. W.

doi:10.1128/jb.75.4.403-408.1958

Cited by 124 publications

(32 citation statements)

References 7 publications

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“…The degradation of cellulose and straw in the presence of combined nitrogen and under a range of oxygen concentrations was followed in liquid culture. A mineral salts medium (11) amended with FeNaEDTA (1.64% [wt/vol] aqueous, 4 ml/liter), dialyzed yeast extract (0.5 g/liter), and sterile phosphate buffer (KH2PO4-NaOH [pH 7.0], final concentration of 100 mM) was dispensed (20 ml per flask) into sterile 125-ml Erlenmeyer flasks containing 100 mg of cellulose (Avicel). The yeast extract was dialyzed to remove yeast cell walls, and the low-molecular-weight components were concentrated in a rotary evaporator before use.…”

Section: Methodsmentioning

confidence: 99%

Cellulose Decomposition and Associated Nitrogen Fixation by Mixed Cultures of Cellulomonas gelida and Azospirillum Species or Bacillus macerans

Halsall

Gibson

1985

Appl Environ Microbiol

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Mixed cultures of Cellulomonas gelida plus Azospirillum lipoferum or Azospirillum brasilense and C. gelida plus Bacillus macerans were shown to degrade cellulose and straw and to utilize the energy-yielding products to fix atmospheric nitrogen. This cooperative process was followed over 30 days in sand-based cultures in which the breakdown of 20% of the cellulose and 28 to 30% of the straw resulted in the fixation of 12 to 14.6 mg of N per g of cellulose and 17 to 19 mg of N per g of g straw consumed. Cellulomonas species have certain advantages over aerobic cellulose-degrading fungi in being able to degrade cellulose at oxygen concentrations as low as 1% O 2 (vol/vol) which would allow a close association between cellulose-degrading and microaerobic diazotrophic microorganisms. Cultures inoculated with initially different proportions of A. brasilense and C. gelida all reached a stable ratio of approximately 1 Azospirillum /3 Cellulomonas cells.

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Section: Methodsmentioning

confidence: 99%

Cellulose Decomposition and Associated Nitrogen Fixation by Mixed Cultures of Cellulomonas gelida and Azospirillum Species or Bacillus macerans

Halsall

Gibson

1985

Appl Environ Microbiol

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“…Putative nitrogen fixers were isolated on 15 ml agar .slants of a nitrogen deficient, Hino and Wilson (1957) medium in 55 ml test tubes sealed with rubber septums ('Suba seals'). The isolates were initially cultured at 28 ± l°C for 48 h under an 80% Nj/20% He atmosphere.…”

Section: Methodsmentioning

confidence: 99%

Nitrogen Fixation Associated with Sand Grain Root Sheaths (Rhizosheaths) of Certain Xeric Grasses

Wullstein

Bruening

Bollen

1979

Physiologia Plantarum

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Nitrogen fixation (acetylene reduction) was found to be associated with sand erain root sheaths (rhizoshcalhs) occurring on the following xeric grasses: Oryzopsis hymcnoiilcs (Rocm. and Shult.) Ricker, Agropyron dasyslachyum (Hook.) Scrib., Stipa comata Trin. and Rupr., and Aristidu purpurva Nutt. Acetylene reduction rates associated with whole plant specimens of these species varied from 515 to 920 nmol C^H^Ag dry wt.) x (6 days). Nitrogeniisc activity was shown to be associated with the rhizo sheaths. Bacillus potymyxa\\)/ic nitrogen fixers were isolated from the rhizosheaths of each grass. The isolates reduced acetylene and assimilated "N,.

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“…Bacillus C-11-25 was grown in modified Hino and Wilson's medium (Hino & Wilson 1957) and A. brasilense was grown in Kalinskaya's medium (Biggins & Postgate 1969). Bacterial suspensions were concentrated by centrifugation and resuspended in sterile phosphate buffer twice to make final cell concentrations of ca 108/mI.…”

Section: G R O W T H P O U C H E X P E R I M E N T Smentioning

confidence: 99%

Plant growth‐altering effects of Azospirillum brasilense and Bacillus C–11–25 on two wheat cultivars

Kucey¹

1988

Journal of Applied Bacteriology

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The effect of Azospirillum brasilense Cd, Bacillus C–11–25, indole acetic acid, gibberellic acid and cytokinin on plant growth characteristics of two wheat (Triticum aestivum L. emend Thell) cultivars was studied under laboratory and greenhouse conditions. Responses of wheat plants to bacterial inoculation were similar to those caused by the addition of gibberellic acid in growth pouches. Chester and Fielder wheat varieties differed in responses to the bacteria and hormone additions. When added to growth pouches, bacterial culture filtrates and dead bacterial cells caused plant growth responses similar to those caused by the addition of live cells. Bacteria and hormone additions resulted in increased permeability of Fielder wheat to 15Nlabelled nitrate, and decreased nitrate permeability of Chester wheat. Bacterial inoculation of soil in pots caused 15N isotope dilution in Fielder but not in Chester wheat. Hormone addition to pots caused isotope dilution in Chester wheat. It appeared that genetic differences between cultivars affected plant growth responses. The accuracy of estimates of N2 fixation by associative bacteria based on 15N isotope dilution calculations may be reduced if control plants differ in plant response to these bacteria.

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Nitrogen Fixation by a Facultative Bacillus

Cited by 124 publications

References 7 publications

Cellulose Decomposition and Associated Nitrogen Fixation by Mixed Cultures of Cellulomonas gelida and Azospirillum Species or Bacillus macerans

Cellulose Decomposition and Associated Nitrogen Fixation by Mixed Cultures of Cellulomonas gelida and Azospirillum Species or Bacillus macerans

Nitrogen Fixation Associated with Sand Grain Root Sheaths (Rhizosheaths) of Certain Xeric Grasses

Plant growth‐altering effects of Azospirillum brasilense and Bacillus C–11–25 on two wheat cultivars

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