Nitrogen-doped carbon nanoparticle modulated turn-on fluorescent probes for histidine detection and its imaging in living cells

Zhu, Xiaohua; Zhao, Tingbi; Nie, Zhou; Miao, Zhuang; Liu, Yang; Yao, Shouzhuo

doi:10.1039/c5nr07826a

Cited by 98 publications

(41 citation statements)

References 63 publications

(67 reference statements)

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“…This result supports the notion that for CNDs the nature of the surface states determines the emission wavelength. The highest PLQYs for N-CND 1 of 17% (10X TAE + starch) and for N-CND 2 of 28% (50X TAE) were obtained for a reaction temperature of 230 °C, which is lower than the recently reported PLQY for an alginate/tryptophan mixture (47.9%)32, but higher than the PLQY values previously reported for N-CNDs obtained from citric acid and urea (14%)35, EDTA (17.5%)34, and waste chicken eggshell and urea (7.8%)23. The strong linearity between PLQY and synthesis temperature (Fig.…”

Section: Resultscontrasting

confidence: 70%

White carbon: Fluorescent carbon nanoparticles with tunable quantum yield in a reproducible green synthesis

Meiling

Cywiński

Bald

2016

Sci Rep

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In this study, a new reliable, economic, and environmentally-friendly one-step synthesis is established to obtain carbon nanodots (CNDs) with well-defined and reproducible photoluminescence (PL) properties via the microwave-assisted hydrothermal treatment of starch and Tris-acetate-EDTA (TAE) buffer as carbon sources. Three kinds of CNDs are prepared using different sets of above mentioned starting materials. The as-synthesized CNDs: C-CND (starch only), N-CND 1 (starch in TAE) and N-CND 2 (TAE only) exhibit highly homogenous PL and are ready to use without need for further purification. The CNDs are stable over a long period of time (>1 year) either in solution or as freeze-dried powder. Depending on starting material, CNDs with PL quantum yield (PLQY) ranging from less than 1% up to 28% are obtained. The influence of the precursor concentration, reaction time and type of additives on the optical properties (UV-Vis absorption, PL emission spectrum and PLQY) is carefully investigated, providing insight into the chemical processes that occur during CND formation. Remarkably, upon freeze-drying the initially brown CND-solution turns into a non-fluorescent white/slightly brown powder which recovers PL in aqueous solution and can potentially be applied as fluorescent marker in bio-imaging, as a reduction agent or as a photocatalyst.

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Section: Resultscontrasting

confidence: 70%

White carbon: Fluorescent carbon nanoparticles with tunable quantum yield in a reproducible green synthesis

Meiling

Cywiński

Bald

2016

Sci Rep

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“…3. In the presence of Cu 2+ , the as-prepared N-CDs with strong fluorescence (curve a) were efficiently quenched (curve b), suggesting that an effective electron or energy transfer process have happened between Cu 2+ and N-CDs 49, 50 . This transfer process was mainly caused by the chelation of Cu 2+ with N-CDs, which could be ascribed to the quite high thermodynamics affinity of Cu 2+ for typical N,O-chelate ligands on the surface of the N-CDs and rapid metal-to-ligand binding kinetics 51, 52 .…”

Section: Resultsmentioning

confidence: 99%

Nitrogen-doped Carbon Dots Mediated Fluorescent on-off Assay for Rapid and Highly Sensitive Pyrophosphate and Alkaline Phosphatase Detection

Geng

Zhang

et al. 2017

Sci Rep

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In this report, a novel fluorescent sensing platform using nitrogen-doped carbon dots (N-CDs) as probes for fluorescence signal transmission has been designed for the detection of significant biomolecules pyrophosphate (PPi) and alkaline phosphatase (ALP). The high fluorescent N-CDs could be selectively quenched by Cu2+, and recovered by the addition of PPi because PPi preferentially binds to Cu2+. Once ALP was introduced into the system, ALP can specifically hydrolyze PPi into Pi, the intense fluorescence of N-CDs could be quenched again due to the recombination of the as-released Cu2+ with N-CDs. So, fluorescence of N-CDs is regulated by an ALP-triggered reaction. Based on this strategy, we demonstrated that N-CDs could serve as a very effective fluorescent sensing platform for label-free, sensitive and selective detection of PPi and ALP with low detection limit of 0.16 μM and 0.4 U/L for PPi and ALP, respectively. Moreover, the assay time is just around 0.5 min for PPi and 30 min for ALP. This developed strategy shows remarkable advantages including sensitive, rapid, simple, convenient, and low-cost and so forth. Furthermore, this method was also successfully applied to monitor ALP in human serum, which indicates its great potential for practical applications in biological and clinical diagnosis.

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“…Interaction between antioxidants and ROS is mainly by hydrogen atom transfer (HAT), single electron transfer (SET), and transition metal chelation [8,9]. As a quasi-zero-dimensional carbon nanomaterial, graphene quantum dots (GQDs) have stable fluorescence [10][11][12], water solubility [13], excellent biocompatibility [14,15], and low cytotoxicity [16][17][18]. Thus, it exhibits great application potential in the fields of bio-imaging [19], catalysis [20,21], desalination [22][23][24], plasmonics [25][26][27][28], and sensing [29].…”

Section: Introductionmentioning

confidence: 99%

Antioxidant Activity of Graphene Quantum Dots Prepared in Different Electrolyte Environments

Zhao

Wang

2019

Nanomaterials

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Antioxidants can reduce or inhibit damage such as oxidative decay caused by elevated levels of free radicals. Therefore, pursuing antioxidants with excellent properties has attracted more and more attention. Graphene quantum dots (GQDs) are considered a promising material because of their good free radical scavenging activity, low toxicity, and excellent water solubility. However, their scavenging efficiency, antioxidant mechanism, and effective control methods need to be improved. Herein, in order to further reveal the antioxidant mechanism of GQDs, the role of electrolytes in improving the antioxidant activity of GQDs is explored. In addition, 1,1-diphenyl-2-picrazine (DPPH∙), hydroxyl (∙OH), and superoxide (∙O2−) free radicals are used to evaluate the antioxidant activity of the as-prepared GQDs. Combined with transmission electron microscopy, Fourier-transform infrared spectroscopy, Raman spectroscopy, and cyclic volt–ampere characteristic curves, the effects of an electrolytic environment on the surface functional groups, charge transfer capability, and defect states of GQDs are obtained. The antioxidant mechanism of GQDs and how to improve their antioxidant activity are further elucidated.

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Nitrogen-doped carbon nanoparticle modulated turn-on fluorescent probes for histidine detection and its imaging in living cells

Cited by 98 publications

References 63 publications

White carbon: Fluorescent carbon nanoparticles with tunable quantum yield in a reproducible green synthesis

White carbon: Fluorescent carbon nanoparticles with tunable quantum yield in a reproducible green synthesis

Nitrogen-doped Carbon Dots Mediated Fluorescent on-off Assay for Rapid and Highly Sensitive Pyrophosphate and Alkaline Phosphatase Detection

Antioxidant Activity of Graphene Quantum Dots Prepared in Different Electrolyte Environments

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