Nitrogen depletion in Arthrospira sp. PCC 8005, an ultrastructural point of view

Deschoenmaeker, Frédéric; Facchini, Raphaël; Cabrera, Juan Carlos; Bayon-Vicente, Guillaume; Sachdeva, Neha; Flammang, Patrick; Wattiez, Ruddy

doi:10.1016/j.jsb.2016.08.007

Cited by 17 publications

(15 citation statements)

References 45 publications

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“…For example, in the case of the action of heavy metals (Cd), first of all, the disintegration and disorganization of thylakoid membranes, as well as the appearance of polyphosphate bodies [ 48 ] are observed. Destruction of photosynthetic membranes and storage of carbon reserves in the form of polyhydroxyalkanoates was also observed in spirulina under conditions of insufficient nutrients, especially nitrogen [ 49 ].…”

Section: Resultsmentioning

confidence: 99%

Modification of Some Structural and Functional Parameters of Living Culture of Arthrospira platensis as the Result of Selenium Nanoparticle Biosynthesis

et al. 2023

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Selenium nanoparticles are attracting the attention of researchers due to their multiple applications, including medicine. The biosynthesis of selenium nanoparticles has become particularly important due to the environmentally friendly character of the process and special properties of the obtained particles. The possibility of performing the biosynthesis of selenium nanoparticles via the living culture of Arthrospira platensis starting from sodium selenite was studied. The bioaccumulation capacity of the culture, along with changes in the main biochemical parameters of the biomass, the ultrastructural changes in the cells during biosynthesis and the change in the expression of some genes involved in stress response reactions were determined. Protein, lipid and polysaccharide fractions were obtained from the biomass grown in the presence of sodium selenite. The formation of selenium nanoparticles in the protein fraction was demonstrated. Thus, Arthrospira platensis culture can be considered a suitable matrix for the biosynthesis of selenium nanoparticles.

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Section: Resultsmentioning

confidence: 99%

Modification of Some Structural and Functional Parameters of Living Culture of Arthrospira platensis as the Result of Selenium Nanoparticle Biosynthesis

et al. 2023

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“…Different strategies have been employed to improve the yield. PHB yield improvement was seen with nitrogen starvation (Deschoenmaeker et al, 2016), phosphate limitation (3.5% of cdw) (Panda et al, 2005), acetate supplement (2.5-3% of cdw) (De Philippis et al, 1992) increased salinity (14.7%) (Shrivastav et al, 2010). Highest yields were obtained with Spirulina sp.…”

Section: Arthrospiramentioning

confidence: 99%

Challenges and Perspectives of Polyhydroxyalkanoate Production From Microalgae/Cyanobacteria and Bacteria as Microbial Factories: An Assessment of Hybrid Biological System

Afreen

Tyagi

Singh

et al. 2021

Front. Bioeng. Biotechnol.

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Polyhydroxyalkanoates (PHAs) are the biopolymer of choice if we look for a substitute of petroleum-based non-biodegradable plastics. Microbial production of PHAs as carbon reserves has been studied for decades and PHAs are gaining attention for a wide range of applications in various fields. Still, their uneconomical production is the major concern largely attributed to high cost of organic substrates for PHA producing heterotrophic bacteria. Therefore, microalgae/cyanobacteria, being photoautotrophic, prove to have an edge over heterotrophic bacteria. They have minimal metabolic requirements, such as inorganic nutrients (CO2, N, P, etc.) and light, and they can survive under adverse environmental conditions. PHA production under photoautotrophic conditions has been reported from cyanobacteria, the only candidate among prokaryotes, and few of the eukaryotic microalgae. However, an efficient cultivation system is still required for photoautotrophic PHA production to overcome the limitations associated with (1) stringent management of closed photobioreactors and (2) optimization of monoculture in open pond culture. Thus, a hybrid system is a necessity, involving the participation of microalgae/cyanobacteria and bacteria, i.e., both photoautotrophic and heterotrophic components having mutual interactive benefits for each other under different cultivation regime, e.g., mixotrophic, successive two modules, consortium based, etc. Along with this, further strategies like optimization of culture conditions (N, P, light exposure, CO2 dynamics, etc.), bioengineering, efficient downstream processes, and the application of mathematical/network modeling of metabolic pathways to improve PHA production are the key areas discussed here. Conclusively, this review aims to critically analyze cyanobacteria as PHA producers and proposes economically sustainable production of PHA from microbial autotrophs and heterotrophs in “hybrid biological system.”

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“…Regarding PHB synthesis, it should be kept in mind that cyanobacteria also produce glycogen as a second carbon and energy storage compound under nitrogen depletion. In fact, the glycogen content is most often higher than the PHB content and varies between 20% and 60% [ 24 , 25 , 26 , 27 , 28 ]. While PHB is produced in 3–8 larger granules, glycogen is stored in many small granules [ 18 , 29 , 30 , 31 , 32 , 33 ].…”

Section: Cyanobacteria and Cyanobacterial Energy And Carbon Storagmentioning

confidence: 99%

“…Under nitrogen depleted conditions PHB granules were more abundant and larger. The nitrogen-starved cells showed an estimated four times higher PHB concentration [ 27 ]. Nitrogen starvation was performed in Arthrospira maxima and glycogen and PHB content was measured.…”

Section: Different Cyanobacteria As Pha Producersmentioning

confidence: 99%

“…Most Arthrospira species produce PHB only in amounts of lower than 5%, even with the addition of sodium acetate. Arthrospira produces glycogen as storage compound, what has been shown in ultrastructural research, too [ 27 ]. Nevertheless, it must be emphasized that Arthrospira , at an industrial scale, is still one of the most promising candidates for PHB production with cyanobacteria.…”

Section: Different Cyanobacteria As Pha Producersmentioning

confidence: 99%

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Cyanobacterial PHA Production—Review of Recent Advances and a Summary of Three Years’ Working Experience Running a Pilot Plant

2017

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Cyanobacteria, as photoautotrophic organisms, provide the opportunity to convert CO2 to biomass with light as the sole energy source. Like many other prokaryotes, especially under nutrient deprivation, most cyanobacteria are able to produce polyhydroxyalkanoates (PHAs) as intracellular energy and carbon storage compounds. In contrast to heterotrophic PHA producers, photoautotrophic cyanobacteria do not consume sugars and, therefore, do not depend on agricultural crops, which makes them a green alternative production system. This review summarizes the recent advances in cyanobacterial PHA production. Furthermore, this study reports the working experience with different strains and cultivating conditions in a 200 L pilot plant. The tubular photobioreactor was built at the coal power plant in Dürnrohr, Austria in 2013 for direct utilization of flue gases. The main challenges were the selection of robust production strains, process optimization, and automation, as well as the CO2 availability.

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Nitrogen depletion in Arthrospira sp. PCC 8005, an ultrastructural point of view

Cited by 17 publications

References 45 publications

Modification of Some Structural and Functional Parameters of Living Culture of Arthrospira platensis as the Result of Selenium Nanoparticle Biosynthesis

Modification of Some Structural and Functional Parameters of Living Culture of Arthrospira platensis as the Result of Selenium Nanoparticle Biosynthesis

Challenges and Perspectives of Polyhydroxyalkanoate Production From Microalgae/Cyanobacteria and Bacteria as Microbial Factories: An Assessment of Hybrid Biological System

Cyanobacterial PHA Production—Review of Recent Advances and a Summary of Three Years’ Working Experience Running a Pilot Plant

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