Nitrogen depletion and small R3-MYB transcription factors affecting anthocyanin accumulation in Arabidopsis leaves

Nemie-Feyissa, Dugassa; Olafsdottir, Solveig Margret; Heidari, Behzad Shiroud; Lillo, Cathrine

doi:10.1016/j.phytochem.2013.12.006

Cited by 84 publications

(49 citation statements)

References 39 publications

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“…One volume of distilled, deionized water was added to 500 mL of extract and mixed. The sample was extracted with 1 mL of chloroform and centrifuged at 13,000 rpm for 5 min (Nemie-Feyissa et al, 2014). Anthocyanins were measured in the aqueous upper phase at 530 nm (modified from Martin et al, 2002).…”

Section: Phytochemical Extraction and Assaysmentioning

confidence: 99%

“…In addition to subgroup 4 R2R3-MYB repressors, the Arabidopsis single-repeat R3-MYB repressors CAPRICE and ENHANCER OF TRYPTOCHON AND CAPRICE (ETC1) were shown to be involved in the downregulation of anthocyanin biosynthesis (Zhu et al, 2009;Nemie-Feyissa et al, 2014). In petunia, the R3-MYB factor MYBx acts as a negative regulator of anthocyanin accumulation in parallel to PhMYB27 (Albert et al, 2014).…”

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confidence: 99%

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The MYB182 Protein Down-Regulates Proanthocyanidin and Anthocyanin Biosynthesis in Poplar by Repressing Both Structural and Regulatory Flavonoid Genes

2015

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Trees in the genus Populus (poplar) contain phenolic secondary metabolites including the proanthocyanidins (PAs), which help to adapt these widespread trees to diverse environments. The transcriptional activation of PA biosynthesis in response to herbivory and ultraviolet light stress has been documented in poplar leaves, and a regulator of this process, the R2R3-MYB transcription factor MYB134, has been identified. MYB134-overexpressing transgenic plants show a strong high-PA phenotype. Analysis of these transgenic plants suggested the involvement of additional MYB transcription factors, including repressor-like MYB factors. Here, MYB182, a subgroup 4 MYB factor, was found to act as a negative regulator of the flavonoid pathway. Overexpression of MYB182 in hairy root culture and whole poplar plants led to reduced PA and anthocyanin levels as well as a reduction in the expression of key flavonoid genes. Similarly, a reduced accumulation of transcripts of a MYB PA activator and a basic helix-loop-helix cofactor was observed in MYB182-overexpressing hairy roots. Transient promoter activation assays in poplar cell culture demonstrated that MYB182 can disrupt transcriptional activation by MYB134 and that the basic helix-loop-helix-binding motif of MYB182 was essential for repression. Microarray analysis of transgenic plants demonstrated that down-regulated targets of MYB182 also include shikimate pathway genes. This work shows that MYB182 plays an important role in the fine-tuning of MYB134-mediated flavonoid metabolism.

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Section: Phytochemical Extraction and Assaysmentioning

confidence: 99%

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confidence: 99%

The MYB182 Protein Down-Regulates Proanthocyanidin and Anthocyanin Biosynthesis in Poplar by Repressing Both Structural and Regulatory Flavonoid Genes

2015

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“…The switching mechanism of a dimerization domain in a bHLH factor to permit distinct configurations of a regulatory complex for binding on different promoters was also reported (Kong et al, 2012). To add to the complexity of MBW activity regulation, several anthocyanin repressors including small R3 MYBs (CPC, ETC1, and MYBx), MYBL2 with partial R2 and complete R3 domain, and subgroup 4 R2R3 MYBs (Fa MYB1 and Fc MYB1 from strawberry [Fragaria spp] and Ph MYB27 from petunia [Petunia hybrida]), have been characterized (Aharoni et al, 2001;Dubos et al, 2008;Zhu et al, 2009;Salvatierra et al, 2013;Albert et al, 2014;Nemie-Feyissa et al, 2014). R3 MYBs, which contain only an R3 domain and no repressive motif, are known to function as passive repressors by competing with R2R3 MYB activators for binding to a bHLH partner (Guimil and Dunand, 2006).…”

Section: Introductionmentioning

confidence: 98%

The Transcriptional Repressor MYB2 Regulates Both Spatial and Temporal Patterns of Proanthocyandin and Anthocyanin Pigmentation inMedicago truncatula

et al. 2015

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Accumulation of anthocyanins and proanthocyanidins (PAs) is limited to specific cell types and developmental stages, but little is known about how antagonistically acting transcriptional regulators work together to determine temporal and spatial patterning of pigmentation at the cellular level, especially for PAs. Here, we characterize MYB2, a transcriptional repressor regulating both anthocyanin and PA biosynthesis in the model legume Medicago truncatula. MYB2 was strongly upregulated by MYB5, a major regulator of PA biosynthesis in M. truncatula and a component of MYB-basic helix loop helix-WD40 (MBW) activator complexes. Overexpression of MYB2 abolished anthocyanin and PA accumulation in M. truncatula hairy roots and Arabidopsis thaliana seeds, respectively. Anthocyanin deposition was expanded in myb2 mutant seedlings and flowers accompanied by increased anthocyanin content. PA mainly accumulated in the epidermal layer derived from the outer integument in the M. truncatula seed coat, starting from the hilum area. The area of PA accumulation and ANTHOCYANIDIN REDUCTASE expression was expanded into the seed body at the early stage of seed development in the myb2 mutant. Genetic, biochemical, and cell biological evidence suggests that MYB2 functions as part of a multidimensional regulatory network to define the temporal and spatial pattern of anthocyanin and PA accumulation linked to developmental processes.

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“…The single-repeat MYBs lack the C-terminal activation domain and inhibit the activator complex by replacing the R2R3 MYB-related transcription factors and thereby suppress trichome initiation in adjacent cells. While some of the positive and negative regulators are specific for trichome patterning others are also involved in root hair development, anthocyanin biosynthesis (Nemie-Feyissa et al, 2014), and seed coat mucilage production (Zhang et al, 2003; Song et al, 2009). …”

Section: Regulation Of Trichome Density In Arabidopsis and Other Brasmentioning

confidence: 99%

Molecular basis of natural variation and environmental control of trichome patterning

Hauser

2014

Front. Plant Sci.

120

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Trichomes are differentiated epidermal cells on above ground organs of nearly all land plants. They play important protective roles as structural defenses upon biotic attacks such as herbivory, oviposition and fungal infections, and against abiotic stressors such as drought, heat, freezing, excess of light, and UV radiation. The pattern and density of trichomes is highly variable within natural population suggesting tradeoffs between traits positively affecting fitness such as resistance and the costs of trichome production. The spatial distribution of trichomes is regulated through a combination of endogenous developmental programs and external signals. This review summarizes the current understanding on the molecular basis of the natural variation and the role of phytohormones and environmental stimuli on trichome patterning.

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Nitrogen depletion and small R3-MYB transcription factors affecting anthocyanin accumulation in Arabidopsis leaves

Cited by 84 publications

References 39 publications

The MYB182 Protein Down-Regulates Proanthocyanidin and Anthocyanin Biosynthesis in Poplar by Repressing Both Structural and Regulatory Flavonoid Genes

The MYB182 Protein Down-Regulates Proanthocyanidin and Anthocyanin Biosynthesis in Poplar by Repressing Both Structural and Regulatory Flavonoid Genes

The Transcriptional Repressor MYB2 Regulates Both Spatial and Temporal Patterns of Proanthocyandin and Anthocyanin Pigmentation inMedicago truncatula

Molecular basis of natural variation and environmental control of trichome patterning

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