Nitrogen deficiency modulates carbon allocation to promote nodule nitrogen fixation capacity in soybean

Abstract

Xiaolong Ke and Xuelu Wang designed the experiments; Xiaolong Ke performed most of the experiments; Han Xiao contributed to the vector construction, material collection, and nodulation assays; Yaqi Peng generated the stable transgenic soybean plants; Xue Xia participated in the design of experiments; Xiaolong Ke and Xuelu Wang wrote the manuscript.

Ionomic and proteomic changes highlight the effect of silicon supply on the functioning of Trifolium incarnatum L. nodules subjected to nitrogen starvation

“…This nding is consistent with maintenance of the number, biomass, and density of nodules as well as their nitrogenase abundance in -Si plants after 25 days of N de ciency (D25) compared with D0 (+N) plants (Table 1). This absence of change in nodulation parameters during N-deprivation agrees with a recent study reporting that soybean subjected to Ndeprivation for 25 days had numbers of nodules and a nodule biomass similar to plants fed with 2 mM nitrate (Ke et al 2024). In contrast, N-deprived plants fed with Si (-N+Si) possessed nodules that were bigger, more numerous and contained higher levels of nitrogenase than -N-Si plants, leading to an increase in their total nitrogen supply due to a sharp rise in their atmospheric N 2 xation capacity (from 21.5 to 87.5 mg plant -1 ).…”

“…Indeed, Xia et al 2017 reported that, in soybean, root nodulation was suppressed by high N (> 50 mg L − 1 ) whereas it was accelerated by low N concentrations (< 50 mg L − 1 ). Finally, in a recent study, Ke et al (2024) reported that a total N-deprivation had no effect on root nodulation or nitrogenase activity in soybean. In addition, N 2 xation is also impaired by various abiotic stress conditions such as other macronutrient de ciencies like potassium (Liu et This study aimed to investigate the effects of Si on physiological traits such as shoot and root biomasses, total N and Si content, N 2 xation capacity, nodulation parameters (nodule biomass, number, density and their nitrogenase content) in Trifolium incarnatum L inoculated with Rhizobium leguminosarum bv trifolii and grown under N-deprivation for a period of 25 days.…”