Nitrogen addition decreases methane uptake caused by methanotroph and methanogen imbalances in a Moso bamboo forest

Li, Quan; Peng, Changhui; Zhang, Junbo; Li, Yongfu; Song, Xinzhang

doi:10.1038/s41598-021-84422-3

Cited by 27 publications

(8 citation statements)

References 96 publications

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“…Thus, a higher abundance of mcrA genes can be expected in topsoils. As for methanogens, which are strictly anaerobic organisms, the presence of mcrA genes has previously been shown in oxic soils in anaerobic microniches (Frey et al, 2011;Li et al, 2021). In addition, anaerobic methanotrophs have been reported that can oxidize methane produced by methanogens (Luesken et al, 2012).…”

Section: Differentially Abundant Genes Of C and N-transforming Processesmentioning

confidence: 93%

Shotgun Metagenomics of Deep Forest Soil Layers Show Evidence of Altered Microbial Genetic Potential for Biogeochemical Cycling

Frey

Varliero

et al. 2022

Front. Microbiol.

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Soil microorganisms such as Bacteria and Archaea play important roles in the biogeochemical cycling of soil nutrients, because they act as decomposers or are mutualistic or antagonistic symbionts, thereby influencing plant growth and health. In the present study, we investigated the vertical distribution of soil metagenomes to a depth of 1.5 m in Swiss forests of European beech and oak species on calcareous bedrock. We explored the functional genetic potential of soil microorganisms with the aim to disentangle the effects of tree genus and soil depth on the genetic repertoire, and to gain insight into the microbial C and N cycling. The relative abundance of reads assigned to taxa at the domain level indicated a 5–10 times greater abundance of Archaea in the deep soil, while Bacteria showed no change with soil depth. In the deep soil there was an overrepresentation of genes for carbohydrate-active enzymes, which are involved in the catalyzation of the transfer of oligosaccharides, as well as in the binding of carbohydrates such as chitin or cellulose. In addition, N-cycling genes (NCyc) involved in the degradation and synthesis of N compounds, in nitrification and denitrification, and in nitrate reduction were overrepresented in the deep soil. Consequently, our results indicate that N-transformation in the deep soil is affected by soil depth and that N is used not only for assimilation but also for energy conservation, thus indicating conditions of low oxygen in the deep soil. Using shotgun metagenomics, our study provides initial findings on soil microorganisms and their functional genetic potential, and how this may change depending on soil properties, which shift with increasing soil depth. Thus, our data provide novel, deeper insight into the “dark matter” of the soil.

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Section: Differentially Abundant Genes Of C and N-transforming Processesmentioning

confidence: 93%

Shotgun Metagenomics of Deep Forest Soil Layers Show Evidence of Altered Microbial Genetic Potential for Biogeochemical Cycling

Frey

Varliero

et al. 2022

Front. Microbiol.

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“…The "three stages" concept is primarily determined by the biphasic dose-response relationship between N input and biotic processes, exhibiting a stimulating effect at low doses and a suppressing effect at high doses (referred to as the "hormesis" effect; (Agathokleous et al, 2020). Additionally, the asynchronous responses of methane production and oxidation processes to N input play a role; the hormesis effect leads to the transition from Stage I to subsequent stages, and the transition from Stage II to Stage III occurs due to the lower tolerance of methanotrophs to N input as compared to methanogens (Li et al, 2021). While methanotrophs are generally sensitive to nitrogen addition (Nyerges & Stein, 2009), at least some methanogens (such as hydrogenotrophic methanogens) are tolerant to high N and low soil pH (Horn Marcus et al, 2003).…”

Section: Global Forest Soil Ch 4 Fluxes and The Underlying Environmen...mentioning

confidence: 99%

Suppression of nitrogen deposition on global forest soil CH4 uptake depends on nitrogen status

Cen,

He,

et al. 2024

Preprint

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Methane (CH4) is the second most important atmospheric greenhouse gas (GHG) and forest soils are a significant sink for atmospheric CH4. Uptake of CH4 by global forest soils is affected by nitrogen (N) deposition; clarifying the effect of N deposition helps to reduce uncertainties of the global CH4 budget. However, it remains an unsolved puzzle why N input stimulates soil CH4 flux (RCH4) in some forests while suppressing it in others. Combining previous findings and data from N addition experiments conducted in global forests, we proposed and tested a “stimulating-suppressing-weakening effect” (“three stages”) hypothesis on the changing responses of RCH4 to N input. Specifically, we calculated the response factors (f) of RCH4 to N input for N-limited and N-saturated forests across biomes; the significant changes in f values supported our hypothesis. We also estimated the global forest soil CH4 uptake budget to be approximately 11.2 Tg yr–1. CH4 uptake hotspots were located predominantly in temperate forests. Furthermore, we quantified that current level of N deposition reduced global forest soil CH4 uptake by ~3%. This suppression effect was more pronounced in temperate forests than in tropical or boreal forests, likely due to differences in N status. The proposed “three stages” hypothesis in this study generalizes the diverse effects of N input on RCH4, which could help improve experimental design. Additionally, our findings imply that by regulating N pollution and reducing N deposition, soil CH4 uptake can be significantly increased in the N-saturated forests in tropical and temperate biomes.

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“…Nitrogen addition was found to decrease methane uptake in a natural environment containing methanotrophs and methanogens [100]. For the effect of ammonia on methanotrophs growth, see [101].…”

Section: Bacillus Firmus (Ncimb Strain 13280)mentioning

confidence: 99%

Value-Added Products from Natural Gas Using Fermentation Processes: Fermentation of Natural Gas as Valorization Route, Part 1

Lackner¹,

Drew²,

Bychkova³

et al. 2022

Natural Gas - New Perspectives and Future Developments [Working Title]

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Methanotrophic bacteria can use methane as their only energy and carbon source, and they can be deployed to manufacture a broad range of value-added materials, from single cell protein (SCP) for feed and food applications over biopolymers such as polyhydroxybutyrate (PHB) to value-added building blocks and chemicals. SCP can replace fish meal and soy for fish (aquacultures), chicken and other feed applications, and also become a replacement of meat after suitable treatment, as a sustainable alternative protein. Polyhydroxyalkanoates (PHA) like PHB are a possible alternative to fossil-based thermoplastics. With ongoing and increasing pressure towards decarbonization in many industries, one can assume that natural gas consumption for combustion will decline. Methanotrophic upgrading of natural gas to valuable products is poised to become a very attractive option for owners of natural gas resources, regardless of whether they are connected to the gas grids. If all required protein, (bio)plastics and chemicals were made from natural gas, only 7, 12, 16–32%, and in total only 35–51%, respectively, of the annual production volume would be required. Also, that volume of methane could be sourced from renewable resources. Scalability will be the decisive factor in the circular and biobased economy transition, and it is methanotrophic fermentation that can close that gap.

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Nitrogen addition decreases methane uptake caused by methanotroph and methanogen imbalances in a Moso bamboo forest

Cited by 27 publications

References 96 publications

Shotgun Metagenomics of Deep Forest Soil Layers Show Evidence of Altered Microbial Genetic Potential for Biogeochemical Cycling

Shotgun Metagenomics of Deep Forest Soil Layers Show Evidence of Altered Microbial Genetic Potential for Biogeochemical Cycling

Suppression of nitrogen deposition on global forest soil CH4 uptake depends on nitrogen status

Value-Added Products from Natural Gas Using Fermentation Processes: Fermentation of Natural Gas as Valorization Route, Part 1

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