Nitro substituent caused negative impact on occurrence and development of atherosclerotic plaque by PM2.5-bound polycyclic aromatic compounds

Zhang, Yu; Zhao, JingBo; Hu, Qian; Mao, HongJun; Wang, Ting

doi:10.1016/j.scitotenv.2023.167700

Cited by 1 publication

(1 citation statement)

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“…Each well was supplemented with 200 μL of medium containing the corresponding pollutants, and the medium was changed every 24 h during the incubation period. After culturing for 24, 48, and 72 h under the experimental conditions, the cells were incubated with CCK-8 reagent (10 μL/well) for 4 h [ 38 ]. Following the kit instructions, CCK-8 optical density (OD) absorbance at 450 nm was measured to judge the effect of exhaust particulate matter on cell viability.…”

Section: Methodsmentioning

confidence: 99%

Lipid Dysregulation Induced by Gasoline and Diesel Exhaust Exposure and the Interaction with Age

Gao,

Zhang,

et al. 2024

Toxics

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Limited knowledge exists regarding gasoline and diesel exhaust effects on lipid metabolism. This study collected gasoline and diesel exhaust under actual driving conditions and conducted inhalation exposure on male young and middle-aged C57BL/6J mice for 4 h/day for 5 days to simulate commuting exposure intensity. Additionally, PM2.5 from actual roadways, representing gasoline and diesel vehicles, was generated for exposure to human umbilical vein endothelial cells (HUVECs) and normal liver cells (LO2) for 24, 48, and 72 h to further investigate exhaust particle toxicity. Results showed that diesel exhaust reduced total cholesterol and low-density lipoprotein cholesterol levels in young mice, indicating disrupted lipid metabolism. Aspartate aminotransferase and alanine aminotransferase levels increased by 53.7% and 21.7%, respectively, suggesting potential liver injury. Diesel exhaust exposure decreased superoxide dismutase and increased glutathione peroxidase levels. Cell viability decreased, and reactive oxygen species levels increased in HUVECs and LO2 following exposure to exhaust particles, with dose- and time-dependent effects. Diesel exhaust particles exhibited more severe inhibition of cell proliferation and oxidative damage compared to gasoline exhaust particles. These findings provide novel evidence of the risk of disrupted lipid metabolism due to gasoline and diesel exhaust, emphasizing the toxicity of diesel exhaust.

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Section: Methodsmentioning

confidence: 99%