Nitrite inhibition of aerobic growth of Acinetobacter sp.

Weon, Seung-Yeon; Lee, Chan-Won; Lee, Sang-Ill; Koopman, Ben

doi:10.1016/s0043-1354(02)00185-9

Cited by 66 publications

(25 citation statements)

References 19 publications

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“…Heterotrophic activity can also be severely impeded by FNA and FA (26,40,50), with distinct bacterial species showing highly varying tolerances for these compounds (6,49). While FA and FNA therefore most likely influenced the diversity of heterotrophic microorganisms within the air filter, it is presently unclear to what extent their joint activity and hence the VOC removal are affected as well.…”

Section: Resultsmentioning

confidence: 99%

Distribution and Rate of Microbial Processes in an Ammonia-Loaded Air Filter Biofilm

Juhler

Revsbech

Schramm

et al. 2009

Appl Environ Microbiol

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The in situ activity and distribution of heterotrophic and nitrifying bacteria and their potential interactions were investigated in a full-scale, two-section, trickling filter designed for biological degradation of volatile organics and NH 3 in ventilation air from pig farms. The filter biofilm was investigated by microsensor analysis, fluorescence in situ hybridization, quantitative PCR, and batch incubation activity measurements. In situ aerobic activity showed a significant decrease through the filter, while the distribution of ammonia-oxidizing bacteria (AOB) was highly skewed toward the filter outlet. Nitrite oxidation was not detected during most of the experimental period, and the AOB activity therefore resulted in NO 2 ؊ , accumulation, with concentrations often exceeding 100 mM at the filter inlet. The restriction of AOB to the outlet section of the filter was explained by both competition with heterotrophic bacteria for O 2 and inhibition by the protonated form of NO 2 ؊ , HNO 2 . Product inhibition of AOB growth could explain why this type of filter tends to emit air with a rather constant NH 3 concentration irrespective of variations in inlet concentration and airflow.Emissions of NH 3 , odorous organic gasses, and dust from pig facilities cause significant problems for neighbors and the surrounding natural environment. In Denmark, swine production accounts for 34% of the total atmospheric NH 3 emission, of which 50% originates from pig house emissions (19). Furthermore, multiple volatile and very odorous organic compounds are emitted with animal house exhaust air and constitute a severe nuisance in residential areas (16,31). While biofilters based on wood chips, compost, and peat have proven efficient in removing complex mixtures of volatile organic compounds (VOC) from piggery exhaust air, biotrickling filters have been shown to be efficient in both odor and NH 3 removal (30). In these filters, airborne VOC and NH 3 are taken up by an irrigated biofilm and oxidized by organoheterotrophic and nitrifying bacteria, respectively, resulting in the production of CO 2 , NO 2 Ϫ or NO 3 Ϫ , and microbial biomass (43). The nitrification process, which comprises the two-step oxidation of NH 3 via NO 2 Ϫ to NO 3 Ϫ , is catalyzed by ammonia-oxidizing bacteria (AOB) and ammonia-oxidizing archaea and by nitriteoxidizing bacteria (NOB), respectively (22, 23). However, in many biotrickling filters, NOB activity seems to be absent, resulting in NO 2 Ϫ being the end product of nitrification (29). Waste products often accumulate in biotrickling filters, as the water is recycled many times to minimize wastewater discharge. In particular, NO 2 Ϫ may accumulate to concentrations above 100 mM (29), resulting in high levels of free nitrous acid (FNA, or HNO 2 ), which is inhibitory to many microorganisms (2, 40, 50). As a result of an overall countercurrent air-water flow, FNA, VOC, and NH 3 concentrations are expected to decrease from the filter air inlet toward the outlet, potentially promoting a gradient of microbial p...

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Section: Resultsmentioning

confidence: 99%

Distribution and Rate of Microbial Processes in an Ammonia-Loaded Air Filter Biofilm

Juhler

Revsbech

Schramm

et al. 2009

Appl Environ Microbiol

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“…For example, the growth rate of heterotrophic Acinetobacter spp. was inhibited by 50% in the presence of 6.5 mM NO 2 Ϫ under conditions of a neutral pH (35), which indicates that these species are far less tolerant than YD35. We previously discovered that the heterotrophic strain Ochrobactrum anthropi YD50.2 tolerates up to 100 mM NO 2 Ϫ (15).…”

Section: Fig 6 Effect Of Nomentioning

confidence: 95%

Achromobacter denitrificans Strain YD35 Pyruvate Dehydrogenase Controls NADH Production To Allow Tolerance to Extremely High Nitrite Levels

Doi

Shimizu

Fujita

et al. 2014

Appl Environ Microbiol

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bWe identified the extremely nitrite-tolerant bacterium Achromobacter denitrificans YD35 that can grow in complex medium containing 100 mM nitrite (NO 2 ؊ ) under aerobic conditions. Nitrite induced global proteomic changes and upregulated tricarboxylate (TCA) cycle enzymes as well as antioxidant proteins in YD35. Transposon mutagenesis generated NO 2 ؊ -hypersensitive mutants of YD35 that had mutations at genes for aconitate hydratase and ␣-ketoglutarate dehydrogenase in the TCA cycle and a pyruvate dehydrogenase (Pdh) E1 component, indicating the importance of TCA cycle metabolism to NO 2 ؊ tolerance. A mutant in which the pdh gene cluster was disrupted (⌬pdh mutant) could not grow in the presence of 100 mM NO 2 ؊ . Nitrite decreased the cellular NADH/NAD ؉ ratio and the cellular ATP level. These defects were more severe in the ⌬pdh mutant, indicating that Pdh contributes to upregulating cellular NADH and ATP and NO 2 ؊ -tolerant growth. Exogenous acetate, which generates acetyl coenzyme A and then is metabolized by the TCA cycle, compensated for these defects caused by disruption of the pdh gene cluster and those caused by NO 2؊ . These findings demonstrate a link between NO 2 ؊ tolerance and pyruvate/acetate metabolism through the TCA cycle. The TCA cycle mechanism in YD35 enhances NADH production, and we consider that this contributes to a novel NO 2 ؊ -tolerating mechanism in this strain. Microorganisms in nature are exposed to various stimuli, such as temperature, osmotic pressure, pH, and reactive chemicals, which in excess cause stress through damage to cellular components (1). Microorganisms have diversified detoxification and tolerance mechanisms during the course of evolution to fight such stressors and adapt to various environments, including extremes (2, 3). Nitrite (NO 2 Ϫ ) is in equilibrium with protons in aqueous solution, and its protonated nitrous acid (HNO 2 ) is a powerful oxidant and mutagen that inhibits bacterial activities (4-6). Further protonation and dismutation generate highly unstable nitrosonium cations (NO ϩ ) that in turn generate reactive nitrogen species (RNS) (7, 8). Thus, NO 2 Ϫ causes more severe oxidative stress under acidic conditions. Nitric oxide (NO) is a toxic RNS which the immune systems of vertebrates produce to kill infective bacteria (9, 10). Microorganisms have developed various RNSdetoxifying proteins, such as flavohemoglobin, flavorubredoxin, and peroxiredoxin, to escape the damage caused by RNS (11-13).The microbial denitrification mechanism dissimilates nitrate (NO 3 Ϫ ), which is significant in anaerobic respiration (14). Bacteria denitrify NO 3 Ϫ to gaseous dinitrogen through successive reduction steps to generate NO 2 Ϫ , NO, and nitrous oxide as reaction intermediates that might accumulate, especially if environmental conditions abruptly change. Denitrifying bacteria that can grow in the presence of high NO 2 Ϫ concentrations were isolated from tropical fish aquariums, where NO 3 Ϫ removal is often inadequate, resulting in high levels of accumulated NO 2 ...

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“…It is important to note that nitrite is known to be toxic to aerobic bacteria. It inhibits various physiological activities such as proton-dependent active transport, functional activity of crucial metabolic enzymes and oxygen uptake and oxidative phosphorylation ensuing interferences to energy conservation [37,38]. Furthermore, nitrite accumulation is also detrimental to aquatic life disrupting their respiratory physiological processes including blood oxygen transport [39].…”

Section: Effect Of Benzoate On Denitrificationmentioning

confidence: 99%

Implications of Benzoate Induced Alterations in Cell Morphology and Physiology in Pseudomonas aeruginosa TMR2.13 for Potential Application in Bioremediation and Monitoring Approaches

Sousa¹

2011

J Bioremed Biodegrad

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Nitrite inhibition of aerobic growth of Acinetobacter sp.

Cited by 66 publications

References 19 publications

Distribution and Rate of Microbial Processes in an Ammonia-Loaded Air Filter Biofilm

Distribution and Rate of Microbial Processes in an Ammonia-Loaded Air Filter Biofilm

Achromobacter denitrificans Strain YD35 Pyruvate Dehydrogenase Controls NADH Production To Allow Tolerance to Extremely High Nitrite Levels

Implications of Benzoate Induced Alterations in Cell Morphology and Physiology in Pseudomonas aeruginosa TMR2.13 for Potential Application in Bioremediation and Monitoring Approaches

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