Nitrite exposure may induce infertility in mice

Abstract

In the present study, we investigated the potential of nitrite exposure to induce infertility in mice. Adult female C57BL/6J mice were randomly divided into control and nitrite exposure groups. Subsequently, the rate of mouse infertility was calculated, and pathological changes in ovarian tissues were examined using hematoxylin and eosin staining. In addition, TUNEL staining, immunofluorescent labeling, and western blotting were performed to assess cell apoptosis and oxidative stress response in ovarian tissues from various groups. We observed that nitrite exposure could induce infertility (P<0.05) in mice. High-dose nitrite exposure caused infertility in a time-dependent manner, and two-round exposure induced higher infertility than that one-round exposure (P<0.01). In addition, a higher number of atretic follicles were detected in the ovaries of nitrite-exposed groups than in the control group. Furthermore, TUNEL-positive cells were observed in granulosa cells of atretic follicles, and overexpression of caspase 8, c-Fos, and inducible nitric oxide synthase (iNOS) was detected in ovaries after nitrite exposure (P<0.01), suggesting that cell apoptosis and oxidative stress response were induced following nitrite exposure. Collectively, these findings suggest that nitrite exposure can induce mouse infertility in a time-dependent manner. Oxidative stress response and cell apoptosis are involved in mediating nitrite-induced infertility.

Reproductive Toxicity of Sodium Nitrite and Its Modulation by Ascorbic Acid as An Antioxidant in Pregnant Female Mice

“…Also, in the mouth cavity, bacteria convert nitrate to nitrite, while in the gastrointestinal system, intestinal microflora converts nitrate to nitrite (Kross et al, 1992). Wu, et al (2022) reported that, exposure to 120 mg/kg nitrite in saline as high dose + one pregnancy cycle gavage (from G0 -pup birth) lead to sterility in a time-dependent manner (p<0.05), and 2-round exposure increase sterility than that one-round exposure in adult female mice (p<0.01). Also, mouse sterility can be induced by oxidative stress response and cell apoptosis following the exposure to nitrite suggesting that nitrite exposure in a time-dependent manner.…”

Águas Potáveis: Padrões De Qualidade E Sustentabilidade

“…Regiões de absorção do espectro de UV-Vis para contaminantes de águas. DI CRISTO et al, 2039;FAN et al, 2019;JOHNSON et al, 2019;WHO, 2019;WU et al, 2022;ZHANG et al, 2017b).…”

“…A análise de probabilidade de ocorrência de índices acima de 10 mg L -1 foi da ordem de 47,3%, confirmando o que saúde humana e animal. Em concentrações acima de 10 mg L -1 pode ser fatal para crianças com idades inferiores a seis meses (QUEIROZ, 2004;ZHENG et al, 2018;WU et al, 2022).…”

“…A Síndrome do Bebê Azul também pode ser observada em gestantes e pode provocar deformações congênitas. Em adultos o íon NO3pode desencadear câncer ou gerar problemas na tireoide, dentre outras enfermidades (CÉSAR et al, 2002;MARTÍNEZ, 2018;MATO, 1996;WU et al, 2022).…”

Uso De Biossorventes Na Remoção De Cromo Em Águas Potáveis: Uma Revisão

“…No organismo humano, o nitrato pode formar substâncias químicas da classe das nitrosaminas e nitrosamidas, que são tóxicas. Então, a ingestão prolongada de águas contaminadas com elevados índices de nitrato, pode provocar problemas na tireoide, desencadear câncer e na gestação, pode provocar deformações congênitas, dentre outras complicações fisológicas (CÉSAR et al, 2002;MARTÍNEZ, 2018;MATO, 1996;WU et al, 2022).…”