Nitrite correlates with 3-nitrotyrosine but not with the F2-isoprostane 15(S)-8-iso-PGF2α in urine of rheumatic patients

Pham, Vu Vi; Stichtenoth, Dirk O.; Tsikas, Dimitrios

doi:10.1016/j.niox.2009.09.001

Cited by 26 publications

(20 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Dimethylamine (rϭ0.66; Pϭ0.07) and ADMA (rϭϪ0.79; Pϭ0.02) correlated with 3-nitrotyrosine ( Figure B), a potential biomarker of myeloperoxidase activity. 2 These findings support the conclusion by Antoniades et al 1 that ADMA may be both a link between inflammation and endothelial dysfunction and a potential therapeutic strategy for the treatment of inflammation-related endothelial dysfunction.…”

Section: Asymmetrical Dimethylarginine Oxidative Stress and Atherossupporting

confidence: 86%

“…In patients with chronic inflammatory rheumatic diseases, serum nitrite concentrations were lower compared with healthy subjects but higher in the patients' synovial fluid, suggesting impaired endothelial NO synthase activity but increased inducible NO synthase activity in the inflamed joint. 2 In coronary artery disease patients without rheumatoid arthritis, 3 as well as in rheumatoid arthritis patients without coronary artery disease, both ADMA synthesis and dimethylarginine dimethylaminohydrolase activity are severalfold elevated compared with healthy controls ( Figure A). Dimethylamine (rϭ0.66; Pϭ0.07) and ADMA (rϭϪ0.79; Pϭ0.02) correlated with 3-nitrotyrosine ( Figure B), a potential biomarker of myeloperoxidase activity.…”

Section: Asymmetrical Dimethylarginine Oxidative Stress and Atherosmentioning

confidence: 99%

See 1 more Smart Citation

Asymmetrical Dimethylarginine, Oxidative Stress, and Atherosclerosis

Chobanyan-Jürgens¹,

Pham²,

Stichtenoth³

et al. 2011

Hypertension

Self Cite

View full text Add to dashboard Cite

Section: Asymmetrical Dimethylarginine Oxidative Stress and Atherossupporting

confidence: 86%

Section: Asymmetrical Dimethylarginine Oxidative Stress and Atherosmentioning

confidence: 99%

Asymmetrical Dimethylarginine, Oxidative Stress, and Atherosclerosis

Chobanyan-Jürgens¹,

Pham²,

Stichtenoth³

et al. 2011

Hypertension

Self Cite

View full text Add to dashboard Cite

“…Mild bacteriuria may be an explanation for the elevated nitrite excretion rate in the urine of phenylketonuria children. On the other hand, elevated excretion rate of nitrite was found in adult patients with rheumatism and may indicate enhanced myeloperoxidase activity (Pham et al 2009). …”

Section: Discussionmentioning

confidence: 99%

Asymmetric dimethylarginine in children with homocystinuria or phenylketonuria

et al. 2011

Self Cite

View full text Add to dashboard Cite

Plasma concentration of asymmetric dimethylarginine (ADMA), an endogenous inhibitor of nitric oxide (NO) synthesis from L-arginine and a cardiovascular risk factor, was found to be elevated in plasma of homocysteinemic adults. Enhanced cardiovascular risk due to homocystinuria and impaired renal function has been found in patients with phenylketonuria (PKU) on protein-restricted diet. However, it is still unknown whether ADMA synthesis is also elevated in children with homocystinuria due to cystathionine beta-synthase deficiency (classical homocystinuria), and whether ADMA may play a role in phenylketonuria in childhood. In the present study, we investigated the status of the L-arginine/NO pathway in six young patients with homocystinuria, in 52 young phenylketonuria patients on natural protein-restricted diet, and in age- and gender-matched healthy children serving as controls. ADMA in plasma and urine was determined by GC-MS/MS. The NO metabolites nitrate and nitrite in plasma and urine, and urinary dimethylamine (DMA), the dimethylarginine dimethylaminohydrolase (DDAH) metabolite of ADMA, were measured by GC-MS. Unlike urine ADMA excretion, plasma ADMA concentration in patients with homocystinuria was significantly higher than in controls (660±158 vs. 475±77 nM, P=0.035). DMA excretion rate was considerably higher in children with homocystinuria as compared to controls (62.2±24.5 vs. 6.5±2.9 μmol/mmol creatinine, P=0.068), indicating enhanced DDAH activity in this disease. In contrast and unexpectedly, phenylketonuria patients had significantly lower ADMA plasma concentrations compared to controls (512±136 vs. 585±125 nM, P=0.009). Phenylketonuria patients and controls had similar L-arginine/ADMA molar ratios in plasma. Urinary nitrite excretion was significantly higher in phenylketonuria as compared to healthy controls (1.7±1.7 vs. 0.7±1.2 μmol/mmol creatinine, P=0.003). Our study shows that the L-arginine/NO pathway is differently altered in children with phenylketonuria and homocystinuria. Analogous to hyperhomocysteinemic adults, elevated ADMA plasma concentrations could be a cardiovascular risk factor in children with homocystinuria. In phenylketonuria, the L-arginine/NO pathway seems not be altered. Delineation of the role of ADMA in childhood phenylketonuria and homocystinuria demands further investigation.

show abstract

“…In a previous study, we found that in adult patients with rheumatism the excretion rate of nitrite was elevated and correlated closely with 3-nitrotyrosine, a biomarker of oxidative stress (Pham et al 2009). Thus, increased nitrite excretion rate in children with DMD may also be associated with enhanced oxidative/nitrosative stress in this disease, which has been reported to be exacerbated in DMD (Terrill et al 2013).…”

mentioning

confidence: 88%

The l-arginine/NO pathway and homoarginine are altered in Duchenne muscular dystrophy and improved by glucocorticoids

et al. 2015

Self Cite

View full text Add to dashboard Cite

The L-arginine/nitric oxide (L-Arg/NO) pathway regulates endothelial function and may play an important role in the pathogenesis of Duchenne muscular dystrophy (DMD). Yet, this pathway is poorly investigated in children suffering from DMD. Endothelial dysfunction can affect the perfusion of contracting muscles, thus leading to ischemia and hypoxia. In the present study, we tested the hypothesis that reduced NO production due to elevated synthesis of N (G),N (G)-dimethyl-L-arginine (asymmetric dimethylarginine, ADMA), an endogenous inhibitor of NO synthesis, is a possible pathophysiological mechanism for progressive intramuscular muscle ischemia and disturbed endothelial function in children with DMD. Given the possible antagonistic action of homoarginine (hArg) on ADMA, we also analyzed this amino acid. We investigated 55 male patients with DMD and 54 healthy male controls (HC; aged 11.9 ± 4.8 vs. 11.1 ± 4.9 years, mean ± SD). Urinary creatinine and metabolites of the L-Arg/NO pathway were measured in plasma and urine by GC-MS or GC-MS/MS. Urine levels of ADMA and its major urinary metabolite dimethylamine (DMA), nitrite and nitrate (P < 0.001 for all) and hArg (P = 0.002) were significantly higher in DMD patients compared to HC, while the urinary DMA/ADMA molar ratio was lower (P = 0.002). In plasma, nitrate (P < 0.001), hArg (P = 0.002) and the hArg/ADMA ratio (P < 0.001) were lower in DMD than in HC. In plasma, ADMA (631 ± 119 vs. 595 ± 129 nM, P = 0.149), arginine and nitrite did not differ between DMD and HC. In DMD, positive correlations between ADMA, DMA or nitrate excretion and the stage of disease (according to Vignos and Thompson) were found. In DMD patients on steroid medication, lower concentrations of ADMA in plasma, and of DMA, ADMA, nitrate and hArg in urine were observed compared to non-treated patients. The L-Arg/NO pathway is impaired in DMD patients, with the disease progression being clinically negatively correlated with the extent of impairment. One of the underlying mechanisms in DMD may involve insufficient antagonism of ADMA by hArg. Steroids, but not creatine supplementation, seems to improve the L-Arg/NO pathway in DMD.

show abstract

Nitrite correlates with 3-nitrotyrosine but not with the F2-isoprostane 15(S)-8-iso-PGF2α in urine of rheumatic patients

Cited by 26 publications

References 37 publications

Asymmetrical Dimethylarginine, Oxidative Stress, and Atherosclerosis

Asymmetrical Dimethylarginine, Oxidative Stress, and Atherosclerosis

Asymmetric dimethylarginine in children with homocystinuria or phenylketonuria

The l-arginine/NO pathway and homoarginine are altered in Duchenne muscular dystrophy and improved by glucocorticoids

Contact Info

Product

Resources

About