Nitrifying and Denitrifying Microbial Communities in Centralized and Decentralized Biological Nitrogen Removing Wastewater Treatment Systems

Wigginton, Sara; Brannon, E.; Kearns, Patrick J.; Lancellotti, Brittany V.; Cox, Alissa H.; Moseman‐Valtierra, Serena; Loomis, George W.; Amador, José A.

doi:10.3390/w12061688

Cited by 11 publications

(11 citation statements)

References 81 publications

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“…Wigginton et al [22] also found Nitrosomonas and Nitrosospira to be the most prevalent genera across both settings. Nitrosospira is most commonly found in soil environments, which suggests that these bacteria are likely introduced to OWTS through soil that enters treatment train components during the initial system installation as well as during periodic maintenance inspections [22,28]. The overlap in prevalent nitrifiers observed in OWTS between systems in the Wigginton et al [17,22] studies and those in our systems suggests that certain genera dominate the nitrification process in N-removal OWTS.…”

Section: Amoa: Taxonomymentioning

confidence: 88%

“…Although Wigginton et al [17] observed a higher number of ASVs (711) in samples from 38 advanced N-removal OWTS, they also identified Nitrosospira and Nitrosomonas as two of the most common genera present in effluent. Both genera have also been observed in BNR WTPs throughout the world [4,22,26,27]. Wigginton et al [22] also found Nitrosomonas and Nitrosospira to be the most prevalent genera across both settings.…”

Section: Amoa: Taxonomymentioning

confidence: 91%

“…Both genera have also been observed in BNR WTPs throughout the world [4,22,26,27]. Wigginton et al [22] also found Nitrosomonas and Nitrosospira to be the most prevalent genera across both settings. Nitrosospira is most commonly found in soil environments, which suggests that these bacteria are likely introduced to OWTS through soil that enters treatment train components during the initial system installation as well as during periodic maintenance inspections [22,28].…”

Section: Amoa: Taxonomymentioning

confidence: 91%

“…Other groups of organisms may play a role in nitrification in wastewater treatment. For example, Wigginton et al [22] assessed amoA communities in a BNR WTP and nine advanced N-removal OWTS in the Greater Narragansett Bay Watershed and observed relatively low abundances of Nitrospira in the advanced N-removal OWTS. Several other studies have also found Nitrospira in aquatic environments with low concentrations of ammonium [23,24].…”

Section: Amoa: Taxonomymentioning

confidence: 99%

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Influence of Season, Occupancy Pattern, and Technology on Structure and Composition of Nitrifying and Denitrifying Bacterial Communities in Advanced Nitrogen-Removal Onsite Wastewater Treatment Systems

Ross

Wigginton

Cox

et al. 2020

Water

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Advanced onsite wastewater treatment systems (OWTS) use biological nitrogen removal (BNR) to mitigate the threat that N-rich wastewater poses to coastal waterbodies and groundwater. These systems lower the N concentration of effluent via sequential microbial nitrification and denitrification. We used high-throughput sequencing to evaluate the structure and composition of nitrifying and denitrifying bacterial communities in advanced N-removal OWTS, targeting the genes encoding ammonia monooxygenase (amoA) and nitrous oxide reductase (nosZ) present in effluent from 44 advanced systems. We used QIIME2 and the phyloseq package in R to examine differences in taxonomy and alpha and beta diversity as a function of advanced OWTS technology, occupancy pattern (seasonal vs. year-round use), and season (June vs. September). Richness and Shannon’s diversity index for amoA were significantly influenced by season, whereas technology influenced nosZ diversity significantly. Season also had a strong influence on differences in beta diversity among amoA communities, and had less influence on nosZ communities, whereas technology had a stronger influence on nosZ communities. Nitrosospira and Nitrosomonas were the main genera of nitrifiers in advanced N-removal OWTS, and the predominant genera of denitrifiers included Zoogloea, Thauera, and Acidovorax. Differences in taxonomy for each gene generally mirrored those observed in diversity patterns, highlighting the possible importance of season and technology in shaping communities of amoA and nosZ, respectively. Knowledge gained from this study may be useful in understanding the connections between microbial communities and OWTS performance and may help manage systems in a way that maximizes N removal.

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Section: Amoa: Taxonomymentioning

confidence: 88%

Section: Amoa: Taxonomymentioning

confidence: 91%

Section: Amoa: Taxonomymentioning

confidence: 91%

Section: Amoa: Taxonomymentioning

confidence: 99%

See 2 more Smart Citations

Influence of Season, Occupancy Pattern, and Technology on Structure and Composition of Nitrifying and Denitrifying Bacterial Communities in Advanced Nitrogen-Removal Onsite Wastewater Treatment Systems

Ross

Wigginton

Cox

et al. 2020

Water

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“…2) indicateing that processes that produce N2O, such as nitrification and incomplete denitrification, take place to the same extent in both zones. Wigginton et al (2020) found that the structure and composition of communities of ammonia oxidizing and denitrifying bacteria in these two zones in nine advanced N-removal OWTS did not differ significantly, suggesting a similar potential for N2O production via these processes. As was the case for the other two gases, N2O flux was not influenced by home occupancy pattern, mirroring the fact that the structure and composition of the microbial communities of seasonal and year-round systems were similar (Fig.…”

Section: Nitrous Oxide Fluxmentioning

confidence: 92%

Greenhouse gas emissions from advanced nitrogen-removal onsite wastewater treatment systems

Ross

Lancellotti

Brannon

et al. 2020

Science of The Total Environment

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Advanced onsite wastewater treatment systems (OWTS) designed to remove nitrogen from residential wastewater play an important role in protecting environmental and public health. Nevertheless, the microbial processes involved in treatment produce greenhouse gases (GHGs) that contribute to global climate change, including CO2, CH4, N2O. We measured GHG emissions from 27 advanced N-removal OWTS in the towns of Jamestown and Charlestown, Rhode Island, USA, and assessed differences in flux based on OWTS technology, home occupancy (year-round vs. seasonal), and zone within the system (oxic vs. anoxic/hypoxic). We also investigated the relationship between flux and wastewater properties. Flux values for CO2, CH4, and N2O ranged from-0.44 to 61.8,-0.0029 to 25.3, and-0.02 to 0.23 µmol GHG m-2 s-1 , respectively. CO2 and N2O flux varied among technologies, whereas occupancy pattern did not significantly impact any GHG fluxes. CO2 and CH4but not N2Oflux was significantly higher in the anoxic/hypoxic zone than in the oxic zone. Greenhouse gas fluxes in the oxic zone were not related to any wastewater properties. CO2 and CH4 flux from the anoxic/hypoxic zone peaked at ~22-23 o C, and was negatively correlated with dissolved oxygen levels, the latter 3 suggesting that CO2 and CH4 flux result primarily from anaerobic respiration. Ammonium concentration and CH4 flux were positively correlated, likely due to inhibition of CH4 oxidation by NH4 +. N2O flux in the anoxic/hypoxic zone was not correlated to any wastewater property. We estimate that advanced N-removal OWTS contribute 262 g CO2 equivalents capita-1 day-1 , slightly lower than emissions from conventional OWTS. Our results suggest that technology influences CO2 and N2O flux and zone influences CO2 and CH4 flux, while occupancy pattern does not appear to impact GHG flux. Manipulating wastewater properties, such as temperature and dissolved oxygen, may help mitigate GHG emissions from these systems.

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Effects of sulphur amino acids on the size and structure of microbial communities of aerobic granular sludge bioreactors

et al. 2022

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Granular activated sludge has been described as a promising tool in treating wastewater. However, the effect of high concentrations of sulphur amino acids, cysteine and methionine, in the evolution, development and stability of AGS-SBRs (aerobic granular sludge in sequential batch reactors) and their microbial communities is not well-established. Therefore, this study aimed to evaluate microbial communities' size, structure and dynamics in two AGS-SBRs fed with two different concentrations of amino acids (50 and 100 mg L−1 of both amino acids). In addition, the impact of the higher level of amino acids was also determined under an acclimatization or shock strategy. While N removal efficiency decreased with amino acids, the removal of the organic matter was generally satisfactory. Moreover, the abrupt presence of both amino acids reduced even further the removal performance of N, whereas under progressive adaptation, the removal yield was higher. Besides, excellent removal rates of cysteine and methionine elimination were found, in all stages below 80% of the influent values. Generally considered, the addition of amino acids weakly impacts the microbial communities' total abundances. On the contrary, the presence of amino acids sharply modulated the dominant bacterial structures. Furthermore, the highest amino acid concentration under the shock strategy resulted in a severe change in the structure of the microbial community. Acidovorax, Flavobacterium, Methylophilus, Stenotrophomonas and Thauera stood out as the prominent bacteria to cope with the high presence of cysteine and methionine. Hence, the AGS-SBR technology is valuable for treating influents enriched in sulphur Aa inclusively when a shock strategy was used.

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Nitrifying and Denitrifying Microbial Communities in Centralized and Decentralized Biological Nitrogen Removing Wastewater Treatment Systems

Cited by 11 publications

References 81 publications

Influence of Season, Occupancy Pattern, and Technology on Structure and Composition of Nitrifying and Denitrifying Bacterial Communities in Advanced Nitrogen-Removal Onsite Wastewater Treatment Systems

Influence of Season, Occupancy Pattern, and Technology on Structure and Composition of Nitrifying and Denitrifying Bacterial Communities in Advanced Nitrogen-Removal Onsite Wastewater Treatment Systems

Greenhouse gas emissions from advanced nitrogen-removal onsite wastewater treatment systems

Effects of sulphur amino acids on the size and structure of microbial communities of aerobic granular sludge bioreactors

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