Nitrification and Denitrification Gene Abundances in Swine Wastewater Anaerobic Lagoons

Ducey, Thomas F.; Shriner, Anthony D.; Hunt, P. G.

doi:10.2134/jeq2010.0387

Cited by 26 publications

(21 citation statements)

References 65 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Conversely, while L2T and L3B clustered loosely with the two remaining depths from their respective lagoons, when compared to the other lagoon samples, they remained most closely related to the samples collected from the same lagoon. Taken together, these results indicate a degree of mixing amongst the lagoon layers, a finding that is supported by previous studies [5,6].…”

Section: Discussionsupporting

confidence: 91%

“…This was confirmed in a report by Ducey et al [6], that quantified the abundance of nitrification and denitrification genes in the water column of eight anaerobic swine wastewater lagoons. They hypothesized that, though redox conditions tend to favor sulfate-reduction and methanogenesis, nitrification and denitrification could potentially occur in select microenvironments, such as the surfaceeliquid interface, where sufficient oxygen diffusion exists [48].…”

Section: Discussionsupporting

confidence: 77%

“…Prior to DNA extraction a composite sample, consisting of equal volumes from each of four quadrant samples taken at each lagoon depth, was generated as previously described [6]. From this composite sample, a volume of 5 mL was centrifuged at 14,000 Â g for 5 min, and DNA was extracted from the resultant pellet as described previously [23].…”

Section: Dna Extractionmentioning

confidence: 99%

See 2 more Smart Citations

Microbial community analysis of swine wastewater anaerobic lagoons by next-generation DNA sequencing

Ducey

Hunt

2013

Anaerobe

Self Cite

View full text Add to dashboard Cite

a b s t r a c tAnaerobic lagoons are a standard practice for the treatment of swine wastewater. This practice relies heavily on microbiological processes to reduce concentrated organic material and nutrients. Despite this reliance on microbiological processes, research has only recently begun to identify and enumerate the myriad and complex interactions that occur in this microbial ecosystem. To further this line of study, we utilized a next-generation sequencing (NGS) technology to gain a deeper insight into the microbial communities along the water column of four anaerobic swine wastewater lagoons. Analysis of roughly one million 16S rDNA sequences revealed a predominance of operational taxonomic units (OTUs) classified as belonging to the phyla Firmicutes (54.1%) and Proteobacteria (15.8%). At the family level, 33 bacterial families were found in all 12 lagoon sites and accounted for between 30% and 50% of each lagoon's OTUs. Analysis by nonmetric multidimensional scaling (NMS) revealed that TKN, COD, ORP, TSS, and DO were the major environmental variables in affecting microbial community structure. Overall, 839 individual genera were classified, with 223 found in all four lagoons. An additional 321 genera were identified in sole lagoons. The top 25 genera accounted for approximately 20% of the OTUs identified in the study, and the low abundances of most of the genera suggests that most OTUs are present at low levels. Overall, these results demonstrate that anaerobic lagoons have distinct microbial communities which are strongly controlled by the environmental conditions present in each individual lagoon.Published by Elsevier Ltd.

show abstract

Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 77%

Section: Dna Extractionmentioning

confidence: 99%

See 1 more Smart Citation

Microbial community analysis of swine wastewater anaerobic lagoons by next-generation DNA sequencing

Ducey

Hunt

2013

Anaerobe

Self Cite

View full text Add to dashboard Cite

show abstract

“…The relatively high abundance of individual nirS , nirK , nosZ I , and nosZ II genes highlights the important biogeochemical role of denitrification in boreal lake sediments. For comparison, the abundance of individual denitrification genes nirS , nirK , and nosZ have previously been found to range between 0.5 and 6.8% of the 16S rRNA gene abundance in various soil and sediment samples [14, 21, 30, 31]. Bioavailability of copper (Cu) and iron (Fe) can control the expression and activity of nitrite and nitrous oxide reductases.…”

Section: Discussionmentioning

confidence: 99%

Genetic and Environmental Controls on Nitrous Oxide Accumulation in Lakes

et al. 2015

View full text Add to dashboard Cite

We studied potential links between environmental factors, nitrous oxide (N2O) accumulation, and genetic indicators of nitrite and N2O reducing bacteria in 12 boreal lakes. Denitrifying bacteria were investigated by quantifying genes encoding nitrite and N2O reductases (nirS/nirK and nosZ, respectively, including the two phylogenetically distinct clades nosZ I and nosZ II) in lake sediments. Summertime N2O accumulation and hypolimnetic nitrate concentrations were positively correlated both at the inter-lake scale and within a depth transect of an individual lake (Lake Vanajavesi). The variability in the individual nirS, nirK, nosZ I, and nosZ II gene abundances was high (up to tenfold) among the lakes, which allowed us to study the expected links between the ecosystem’s nir-vs-nos gene inventories and N2O accumulation. Inter-lake variation in N2O accumulation was indeed connected to the relative abundance of nitrite versus N2O reductase genes, i.e. the (nirS+nirK)/nosZ I gene ratio. In addition, the ratios of (nirS+nirK)/nosZ I at the inter-lake scale and (nirS+nirK)/nosZ I+II within Lake Vanajavesi correlated positively with nitrate availability. The results suggest that ambient nitrate concentration can be an important modulator of the N2O accumulation in lake ecosystems, either directly by increasing the overall rate of denitrification or indirectly by controlling the balance of nitrite versus N2O reductase carrying organisms.

show abstract

“…Primers used in these assays were synthesized by Integrated DNA Technologies (Coralville, IA) and are listed in Table 3. Assays were carried out using SYBR GreenER qPCR SuperMix (Invitrogen, Carlsbad, CA) in a total reaction volume of 25 L as previously described (Ducey et al, 2011). Final reaction concentrations of reagents consisted of 1× SYBR GreenER qPCR SuperMix, 200 nM each of forward and reverse primers (Table 3), and 1 L of a 1:100 dilution of DNA template.…”

Section: Quantitative Real-time Polymerase Chain Reaction (Qpcr) Assaysmentioning

confidence: 99%

Addition of activated switchgrass biochar to an aridic subsoil increases microbial nitrogen cycling gene abundances

Ducey

Ippolito

Cantrell

et al. 2013

Applied Soil Ecology

Self Cite

177

View full text Add to dashboard Cite

a b s t r a c tIt has been demonstrated that soil amended with biochar, designed specifically for use as a soil conditioner, results in changes to the microbial populations that reside therein. These changes have been reflected in studies measuring variations in microbial activity, biomass, and community structure. Despite these studies, very few experiments have been performed examining microbial genes involved in nutrient cycling processes. Given the paucity of research in this area, we designed a 6 month study in a Portneuf subsoil treated with three levels (1%, 2%, and 10% w/w ratio) of a biochar pyrolyzed from switchgrass (Panicum virgatum) at 350 • C and steam activated at 800 • C to measure the abundances of five genes involved in N cycling. Gene abundances were measured using qPCR, with relative abundances of these genes calculated based on measurement of the 16S rRNA gene. At the end of the 6 month study, all measured genes showed significantly greater abundances in biochar amended treatments as compared to the control. In soil amended with 10% biochar, genes involved in nitrogen fixation (nifH), and denitrification (nirS), showed significantly increased relative abundances. Lastly, gene abundances and relative abundances correlated with soil characteristics, in particular NO 3 -N, % N and % C. These results confirm that activated switchgrass-derived biochar, designed for use as a soil conditioner, has an impact on the treated soils microbial communities. We therefore suggest that future use of biochar as a soil management practice should take into account not only changes to the soil's physiochemical properties, but its biological properties as well.Published by Elsevier B.V.

show abstract

Nitrification and Denitrification Gene Abundances in Swine Wastewater Anaerobic Lagoons

Cited by 26 publications

References 65 publications

Microbial community analysis of swine wastewater anaerobic lagoons by next-generation DNA sequencing

Microbial community analysis of swine wastewater anaerobic lagoons by next-generation DNA sequencing

Genetic and Environmental Controls on Nitrous Oxide Accumulation in Lakes

Addition of activated switchgrass biochar to an aridic subsoil increases microbial nitrogen cycling gene abundances

Contact Info

Product

Resources

About