Nitrification activity and community structure of nitrite-oxidizing bacteria in the bioreactors operated with addition of pharmaceuticals

Kraigher, Barbara; Mandić-Mulec, Ines

doi:10.1016/j.jhazmat.2011.01.072

Cited by 17 publications

(6 citation statements)

References 43 publications

(51 reference statements)

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“…In addition, the increase in the concentration of N-NO 3 - in the soils treated with NSAIDs might result from the stimulation of the ammonification process, as was indicated by the increased concentration of N-NH 4 + , which is the substrate for the nitrifiers (Hicks et al, 1990). The low sensitivity of nitrifiers to NSAIDs was proven by Kraigher and Mandic-Mulec (2011), who observed that the addition of different concentrations (0, 0.05, 0.2, 0.5 mg/L) of IBF, NPX, KTP, and DCF did not affect ammonia removal. However, the eﬄuent concentration of N-NO 2 - and N-NO 3 - was significantly higher in the two reactors that were operated with 0.05 mg/L of NSAIDs than in the other reactors.…”

Section: Discussionmentioning

confidence: 99%

Variable Effects of Non-steroidal Anti-inflammatory Drugs (NSAIDs) on Selected Biochemical Processes Mediated by Soil Microorganisms

et al. 2016

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Non-steroidal anti-inflammatory drugs (NSAIDs) are the most frequently used group of pharmaceuticals. The high consumption and the uncontrolled disposal of unused drugs into municipal waste or their deposit in landfills can result in an increased concentration of these compounds in soils. Moreover, these drugs can affect the microbial activity. However, there is a lack of knowledge about these effects or it is very limited. Therefore, the objective of this study was to compare the impact of selected commercially available NSAIDs, i.e., diclofenac (DCF), naproxen (NPX), ibuprofen (IBF) and ketoprofen (KTP), applied at concentrations of 1 and 10 mg/kg soil, on the activity of soil microorganisms during the 90-day experiment. To ascertain this impact, substrate-induced respiration (SIR), soil enzyme activities, i.e., dehydrogenase (DHA), acid and alkaline phosphatases (PHOS-H and PHOS-OH) and urease (URE) as well as changes in the rates of nitrification and ammonification processes were determined. In addition, the number of culturable bacteria and fungi were enumerated. In general, the obtained data showed a significant stimulatory effect of NSAIDs on the microbial activity. Higher concentrations of NSAIDs caused a greater effect, which was observed for SIR, PHOS-H, PHOS-OH, URE, N-NO3- and N-NH4+, even during the whole incubation period. Moreover, the number of heterotrophic bacteria and fungi increased significantly during the experiment, which was probably a consequence of the evolution of specific microorganisms that were capable of degrading NSAIDs and used them as an additional source of carbon and energy. However, an inhibitory effect of NPX, IBF or KTP for SIR, DHA, on both phosphatases and culturable bacteria and fungi was observed at the beginning of the experiment. At lower concentrations of NSAIDs, in turn, the effects were negligible or transient. In conclusion, the application of NSAIDs altered the biochemical and microbial activity of soil what may cause the disturbance in soil functioning. It is reasonable to assume that some components of the NSAID formulations could stimulate soil microorganisms, thus resulting in an increase in biochemical activities of the soil.

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Section: Discussionmentioning

confidence: 99%

Variable Effects of Non-steroidal Anti-inflammatory Drugs (NSAIDs) on Selected Biochemical Processes Mediated by Soil Microorganisms

et al. 2016

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“…Finally, the highest diversity levels for the phylum Nitrospirae were found in the R40 samples, with several OTUs overrepresented (Figure 6, Table 4). The Nitrospirae are known as chemoautotrophic bacteria involved in the nitrogen cycle and several members are involved in nitrite oxidation while sharing genes with anammox bacteria [114], [115].…”

Section: Discussionmentioning

confidence: 99%

Linking Geology and Microbiology: Inactive Pockmarks Affect Sediment Microbial Community Structure

2014

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Pockmarks are geological features that are found on the bottom of lakes and oceans all over the globe. Some are active, seeping oil or methane, while others are inactive. Active pockmarks are well studied since they harbor specialized microbial communities that proliferate on the seeping compounds. Such communities are not found in inactive pockmarks. Interestingly, inactive pockmarks are known to have different macrofaunal communities compared to the surrounding sediments. It is undetermined what the microbial composition of inactive pockmarks is and if it shows a similar pattern as the macrofauna. The Norwegian Oslofjord contains many inactive pockmarks and they are well suited to study the influence of these geological features on the microbial community in the sediment. Here we present a detailed analysis of the microbial communities found in three inactive pockmarks and two control samples at two core depth intervals. The communities were analyzed using high-throughput amplicon sequencing of the 16S rRNA V3 region. Microbial communities of surface pockmark sediments were indistinguishable from communities found in the surrounding seabed. In contrast, pockmark communities at 40 cm sediment depth had a significantly different community structure from normal sediments at the same depth. Statistical analysis of chemical variables indicated significant differences in the concentrations of total carbon and non-particulate organic carbon between 40 cm pockmarks and reference sample sediments. We discuss these results in comparison with the taxonomic classification of the OTUs identified in our samples. Our results indicate that microbial communities at the sediment surface are affected by the water column, while the deeper (40 cm) sediment communities are affected by local conditions within the sediment.

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“…Biodegradation was observed until all of the ammonia was consumed, suggesting that either the nitrifiers were responsible for CLF biodegradation or heterotrophs were responsible for this transformation during biomass growth. Many previous studies have suggested a co-metabolic action of ammonia and aromatic compounds in nitrifying bacteria [25][26][27][28]. Yi and Harper [27] proposed that the dioxygenase enzymes are capable of mediating the co-metabolic biotransformation of aromatic compounds (for biotransformation of 17␣-ethinylestradiol), which was not only associated with nitrifiers but also to heterotrophic bacteria.…”

Section: Clofibric Acid Biotransformationmentioning

confidence: 99%

Biodegradation of clofibric acid and identification of its metabolites

Salgado

Oehmen

Carvalho

et al. 2012

Journal of Hazardous Materials

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Nitrification activity and community structure of nitrite-oxidizing bacteria in the bioreactors operated with addition of pharmaceuticals

Cited by 17 publications

References 43 publications

Variable Effects of Non-steroidal Anti-inflammatory Drugs (NSAIDs) on Selected Biochemical Processes Mediated by Soil Microorganisms

Variable Effects of Non-steroidal Anti-inflammatory Drugs (NSAIDs) on Selected Biochemical Processes Mediated by Soil Microorganisms

Linking Geology and Microbiology: Inactive Pockmarks Affect Sediment Microbial Community Structure

Biodegradation of clofibric acid and identification of its metabolites

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