Nitric oxide up-regulates the expression of calcium-dependent potassium channels in the supraoptic nuclei and neural lobe of rats following dehydration

Kadekaro, Massako; Su, Guangxiao; Chu, Rong; Lei, Yongzhong; Li, Junfa; Li, Fang

doi:10.1016/j.neulet.2006.05.035

Cited by 11 publications

(16 citation statements)

References 25 publications

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“…Thus, our results suggest that NO may modify the thiol groups of the α subunit of the BK channel to modulate the activity of magnocellular neurons. In accordance with this concept in previous studies, we also reported that dithiothreitol, a thiol-reducing agent, treated icv to water-sated rats increased the plasma levels of vasopressin and oxytocin as well as their localized enhancement of the expression in these neuronal populations (16,17). However, by reducing NO synthesis with L-NAME, this effect was also observed when NO levels were decreased.…”

supporting

confidence: 89%

“…In the magnocellular neurons, NO activates BK channels in the posterior pituitary and depresses the excitability of the terminals. This reduced impulse activity could lead to the inhibitory action of NO on hormone secretion (16,17). Recent findings from our lab indicated that water deprivation significantly upregulated BK channel protein in magnocellular neurons and that NO levels affected this regulation (17).…”

Section: Nih Public Accessmentioning

confidence: 99%

“…As a marker of NOS activity, NADPH-diaphorase was reported to reside in this system (2,23). The activated NO system was reported to involve the response of magnocellular neurons to acute and chronic osmotic insults, such as dehydration and hypovolemia (17,18,30). These studies indicate that disturbances of fluid balance triggered the system to produce NO to meet the increasing demand for NO modulation in the magnocellular system.…”

Section: Introductionmentioning

confidence: 96%

“…In the hypothalamo-neurohypophysial system, NO mediates neuronal synaptic transmission and plasticity in the regulation of vasopressin and oxytocin secretion (16,17). As the important neuroendocrine cells, the supraoptic nuclei (SON) and paraventricular nuclei (PVN), as well as in their axon terminals in the neural lobe, synthesizes vasopressin and oxytocin in response to osmotic alternations in physiological and pathophysiological states (2,16 Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication.…”

Section: Introductionmentioning

confidence: 99%

“…We previously reported that osmotic stimulation significantly increased the NOS activity in the SON and neural lobe in rats (17,18). In several studies, NO was reportedly generated dynamically during conditions of normal hydration to inhibit the secretion of both vasopressin and oxytocin in the neuroendocrine system (5,11,16,21,26).…”

mentioning

confidence: 99%

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Effects of nitric oxide on expressions of nitrosocysteine and calcium-activated potassium channels in the supraoptic nuclei and neural lobe of dehydrated rats

Kadekaro

Chu

et al. 2007

Neuroscience Letters

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Nitric oxide (NO) is an important gas mediator in the signal transduction cascade regulating osmotic function in the hypothalamo-neurohypophysial system. We previously found that increased nitric oxide synthase (NOS) activity in the supraoptic nuclei (SON) and neural lobe following osmotic stimulation and NO could regulate the expression of Ca 2+ -activated K + channel (BK channels) protein in the magnocellular system during dehydration. The aim of the current study is to examine the role of NO in the regulation of nitrosocysteine and BK channel protein in the magnocellular system in dehydrated animals. Using Western blot analysis and quantitative immunofluorescent staining study, we found that water deprivation in rats significantly enhanced the expression of nitrosocysteine protein in SON and neural lobes. Immunohistochemistry study indicated that dehydration significantly increased the profiles of SON neurons co-expressing nitrosocysteine with BK-channel protein. Intracerebroventricular administration of L-NAME (an inhibitor of NO synthase) significantly reduced the neuronal profiles of nitrosocysteine, as well as their co-expression with BK-channel in SON of dehydrated rats. However, treatment of sodium nitroprusside (a donor of NO) increased this co-expression. Our results indicate that NO signaling cascade may control the expression of BK channels through the regulation of nitrosocysteine in SON and neural lobe of rats during osmotic regulation.Nitric oxide (NO), a gas molecule generated endogenously from the amino acid L-arginine by nitric oxide synthase (NOS), is a freely diffusible intercellular messenger that functions in various cells in the nervous system. In the hypothalamo-neurohypophysial system, NO mediates neuronal synaptic transmission and plasticity in the regulation of vasopressin and oxytocin secretion (16,17). As the important neuroendocrine cells, the supraoptic nuclei (SON) and paraventricular nuclei (PVN), as well as in their axon terminals in the neural lobe, synthesizes vasopressin and oxytocin in response to osmotic alternations in physiological and pathophysiological states (2,16 Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form. Please note that during the production process errorsmaybe discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. reported in the entire hypothalamo-neurohypophysial system (18,30). As a marker of NOS activity, NADPH-diaphorase was reported to reside in this system (2,23). The activated NO system was reported to involve the response of magnocellular neurons to acute and chronic osmotic insults, such as dehydration and hypovolemia (17,18,30). These studies indicate that disturbances of fluid balance triggered the system to prod...

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supporting

confidence: 89%

Section: Nih Public Accessmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

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Effects of nitric oxide on expressions of nitrosocysteine and calcium-activated potassium channels in the supraoptic nuclei and neural lobe of dehydrated rats

Kadekaro

Chu

et al. 2007

Neuroscience Letters

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Neuronal Circuits and Neuroendocrine Responses Involved in Dehydration Induced by Water Restriction/Deprivation

Zhu¹,

Wang²,

McTaggart³

et al. 2011

Handbook of Behavior, Food and Nutrition

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BKβ1 Subunits Contribute to BK Channel Diversity in Rat Hypothalamic Neurons

et al. 2010

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Large conductance Ca(2+)-activated BK channels are important regulators of action potential duration and firing frequency in many neurons. As the pore-forming subunits of BK channels are encoded by a single gene, channel diversity is mainly generated by alternative splicing and interaction with auxiliary beta-subunits (BKbeta1-4). In hypothalamic neurons several BK channel subtypes have been described electrophysiologically; however, the distribution of BKbeta subunits is unknown so far. Therefore, an antibody against the large extracellular loop of the BKbeta1 subunit was raised, freed from cross-reactivity against BKbeta2-4 and affinity-purified. The resulting polyclonal monospecific BKbeta1 antibody was characterized by Western blot analysis, ELISA techniques and immunocytochemical staining of BKbeta1-4-transfected CHO and COS-1 cells. Regional and cellular distribution in the rat hypothalamus was analysed by immunocytochemistry and in situ hybridization experiments. Immunocytochemical staining of rat hypothalamic neurons indicates strong BKbeta1 expression in the supraoptic nucleus and the magno- and parvocellular parts of the paraventricular nucleus. Lower expression was found in periventricular nucleus, the arcuate nucleus and in the median eminence. Immunostaining was predominantly localized to somata. In addition, pericytes and ependymal epithelial cells showed BKbeta1 labelling. In all cases immunocytochemical results were supported by in situ hybridization.

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Nitric oxide up-regulates the expression of calcium-dependent potassium channels in the supraoptic nuclei and neural lobe of rats following dehydration

Cited by 11 publications

References 25 publications

Effects of nitric oxide on expressions of nitrosocysteine and calcium-activated potassium channels in the supraoptic nuclei and neural lobe of dehydrated rats

Effects of nitric oxide on expressions of nitrosocysteine and calcium-activated potassium channels in the supraoptic nuclei and neural lobe of dehydrated rats

Neuronal Circuits and Neuroendocrine Responses Involved in Dehydration Induced by Water Restriction/Deprivation

BKβ1 Subunits Contribute to BK Channel Diversity in Rat Hypothalamic Neurons

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