Nitric Oxide Synthesis in the Adult and Developing Kidney

Han, Ki-Hwan; Jung, Jongwan; Chung, Ku Yong; Kim, Hyang; Kim, Jin

doi:10.5049/ebp.2006.4.1.1

Cited by 7 publications

(6 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The abundance of the substrate, L-arginine, is well-maintained during aging, but there are increases in the concentration of circulating endogenous NOS inhibitors, which could contribute to a powerful inhibitory effect in adult normotensive animals and weakened in young animals [44]. In the renal cortex of normotensive rats, both endothelial and neuronal NOS [45] are abundantly expressed. All three isoforms of NOS (nNOS, iNOS, and eNOS) could have a higher expression of the mRNA or protein level in SHR compared to Wistar Kyoto rats [36, 37].…”

Section: Discussionmentioning

confidence: 99%

Chronic NOS Inhibition Affects Oxidative State and Antioxidant Response Differently in the Kidneys of Young Normotensive and Hypertensive Rats

Majzúnová

Kvandová

Berenyiová

et al. 2019

Oxidative Medicine and Cellular Longevity

View full text Add to dashboard Cite

Deficiency of nitric oxide (NO) and oxidative stress can be a cause, a consequence, or, more often, a potentiating factor for hypertension and hypertensive renal disease. Both NO and superoxide anions are radical molecules that interact with each other, leading to oxidative damage of such organs as the kidney. In the present study, we investigated the effect of chronic-specific (neuronal NOS inhibition) and nonspecific NOS inhibition on the oxidative state and antioxidant response and associated oxidative damage of the kidney of young normotensive and hypertensive rats. Young male normotensive Wistar rats (WRs, age 4 weeks) and spontaneously hypertensive rats (SHRs, age 4 weeks) were divided into three groups for each strain by the type of administered compounds. The first group was treated with 7-nitroindazole (WR+7-NI; SHR+7-NI), the second group was treated with N(G)-nitro-L-arginine-methyl ester (WR+L-NAME; SHR+L-NAME), and the control group was treated with pure drinking water (WR; SHR) continuously for up to 6 weeks. Systolic blood pressure increased in WR+L-NAME after the first week of administration and increased slightly in SHR+L-NAME in the third week of treatment. 7-NI had no effect on blood pressure. While total NOS activity was not affected by chronic NOS inhibition in any of the WR groups, it was attenuated in SHR+7-NI and SHR+L-NAME. Nitration of proteins (3-nitrotyrosine expression) was significantly reduced in WR+7NI but not in WR+L-NAME and increased in SHR+7-NI and SHR+L-NAME. Immunoblotting analysis of SOD isoforms showed decreased SOD2 and SOD3 expressions in both WR+7-NI and WR+L-NAME followed by increased SOD activity in WR+L-NAME. Conversely, increased expression of SOD2 and SOD3 was observed in SHR+L-NAME and SHR+7-NI, respectively. SOD1 expression and total activity of SOD did not change in the SHR groups. Our results show that the antioxidant defense system plays an important role in maintaining the oxidative state during NO deficiency. While the functioning antioxidant system seeks to balance the oxidation state in the renal cortex of normotensive WRs, the impaired antioxidant activity leads to the development of oxidative damage of proteins in the kidney induced by peroxynitrite in SHRs.

show abstract

Section: Discussionmentioning

confidence: 99%

Chronic NOS Inhibition Affects Oxidative State and Antioxidant Response Differently in the Kidneys of Young Normotensive and Hypertensive Rats

Majzúnová

Kvandová

Berenyiová

et al. 2019

Oxidative Medicine and Cellular Longevity

View full text Add to dashboard Cite

show abstract

“…Moreover, the present study indicated that iNOS is widely expressed in renal tubule epithelia, including the proximal, distal and collecting tubules. iNOS is known to play significant roles in the regulation of kidney hemodynamics (Han, Jung, Chung, Kim, & Kim, 2006). Wang (2002) has confirmed that iNOS regulates tubular water and electrolytes excretion in the kidney and the tubular reabsorptive function as well as, it has a role in the regulation of renal blood flow.…”

Section: Discussionmentioning

confidence: 99%

The structural and ultrastructural organization of the cellular constituents of the trunk kidney of grass carp (Ctenopharyngodon idella)

Mokhtar

2020

Microscopy Res & Technique

View full text Add to dashboard Cite

The trunk kidney of grass carp mainly consisted of renal tubules with a few interstitial hematopoietic tissues. The component structure of fish nephron markedly varies between different species of fish. The nephron of grass carp consisted of morphologically distinct segments; renal corpuscles, neck segment, proximal, intermediate, distal, and collecting tubules. The glomerulus of renal corpuscles mainly composed of mesangial cells and well‐developed podocytes that extended their processes to the endothelium of glomerular capillaries forming the filtration barrier. The podocytes expressed both α‐SMA and the transforming growth factor gene, TGF‐β. The proximal convoluted tubules (PCTs) expressed α‐SMA iNOS2, and TGF‐β. The cytoplasm of PCT was rich in mitochondria and rER, in addition to the presence of well‐developed basolateral tubular system and apical brush borders. Collecting tubules distributed throughout the kidney and lined by principal and flask cells. The interstitial hemopoietic tissues contained iNOS2‐positive polymorphic granulocytes, CD3‐positive T lymphocytes, rodlet cells, dendritic cells, macrophages, melanomacrophage centers, and telocytes. This study described for the first time the cellular components of the nephron and its associated hemopoietic tissues that can act as a basis for studying the structural changes that may occur in the kidney of grass carp during water salinitiy, environmental, or experimental conditions.

show abstract

“…Its expression was also found in the epithelia of TAL of Henle and CD (180). Whereas nNOS was detected predominantly in the tubular epithelial cells of the macula densa (114) in the kidneys of rat, mouse, guinea pig, and rabbit, the intensity of signal was much weaker in humans and pigs (18). Additionally, nNOS was also detected in inner and outer medullary CD, efferent arterioles, Bowman's capsule, and various cells at the cortical TAL in rats (311).…”

Section: Endothelium-associatedmentioning

confidence: 91%

“…Interestingly, expression of all three isoforms have been detected in the kidney tissues but have different distribution profiles (168). For example, eNOS was found to be expressed in the endothelial cells (ECs) of glomeruli and peritubular capillaries in the cortex, and in the ECs of vascular bundles in the medulla, with a stronger immunoreactivity in the cells of renal medulla than in the cortex (114). Its expression was also found in the epithelia of TAL of Henle and CD (180).…”

Section: Endothelium-associatedmentioning

confidence: 99%

Renal cell markers: lighthouses for managing renal diseases

Agarwal

Sudhini

Polat

et al. 2021

American Journal of Physiology-Renal Physiology

View full text Add to dashboard Cite

Kidneys, one of the vital organs in our body, are responsible for maintaining whole-body homeostasis. The complexity of renal function (e.g., filtration, reabsorption, fluid and electrolyte regulation, urine production) demands diversity not only at the level of cell types but also in their overall distribution and structural framework within the kidney. To gain an in-depth molecular-level understanding of the renal system, it is imperative to discern the components of kidney and the types of cells residing in each of the sub-regions. Recent developments in labeling, tracing, and imaging techniques enabled us to mark, monitor and identify these cells in vivo with high efficiency in a minimally invasive manner. In this review, we have summarized different cell types, specific markers that are uniquely associated with those cell types, and their distribution in kidney, which altogether make kidneys so special and different. Cellular sorting based on the presence of certain proteins on the cell surface allowed for assignment of multiple markers for each cell type. However, different studies using different techniques have found contradictions in the cell-type specific markers. Thus, the term "cell marker" might be imprecise and sub-optimal, leading to uncertainty when interpreting the data. Therefore, we strongly believe that there is an unmet need to define the best cell markers for a cell type. Although, the compendium of renal-selective marker proteins presented in this review is a resource that may be useful to the researchers, we acknowledge that the list may not be necessarily exhaustive.

show abstract

Nitric Oxide Synthesis in the Adult and Developing Kidney

Cited by 7 publications

References 42 publications

Chronic NOS Inhibition Affects Oxidative State and Antioxidant Response Differently in the Kidneys of Young Normotensive and Hypertensive Rats

Chronic NOS Inhibition Affects Oxidative State and Antioxidant Response Differently in the Kidneys of Young Normotensive and Hypertensive Rats

The structural and ultrastructural organization of the cellular constituents of the trunk kidney of grass carp (Ctenopharyngodon idella)

Renal cell markers: lighthouses for managing renal diseases

Contact Info

Product

Resources

About