Nitric oxide synthase neurons in the rodent spinal cord: distribution, relation to Substance P fibers, and effects of dorsal rhizotomy

Reuss, Martina H.; Reuss, Stefan

doi:10.1016/s0891-0618(01)00091-6

Cited by 37 publications

(31 citation statements)

References 77 publications

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“…Anti-bNOS, a marker for autonomic motor neurons located in the IML region of thoracic spinal cord (Reuss and Reuss, 2001), exhibited strong colocalization with anti-␣3, anti-␣5, and anti-␤2. Colocalization of anti-␣4 and anti-␤4 with anti-bNOS was far weaker than with the other three nicotinic receptor subunit antibodies (Fig.…”

Section: Colocalization Of Nicotinic Receptor Subunits With Cellular mentioning

confidence: 99%

Nicotinic acetylcholine receptor distribution in relation to spinal neurotransmission pathways

Khan

Osaka

Stanislaus

et al. 2003

J of Comparative Neurology

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Neuronal nicotinic receptors (nAChR) are pentameric assemblies of subunits of a gene family where specified combinations of alpha and beta subunits form functional receptors. To extend our understanding of the role of spinal nAChR in the processing of sensory stimuli and regulation of autonomic and motor responses, we initiated investigations to localize nAChR subunit expression within discrete spinal regions and cell types. High-affinity epibatidine binding was present in the superficial dorsal and ventral horns, the mediolateral and central canal regions. RT-PCR identified transcripts for alpha3, alpha4, alpha5, beta2, and beta4 in both spinal cord parenchyma and dorsal root ganglia (DRG). Our affinity-purified antibodies against alpha3, alpha4, alpha5, beta2, and beta4 subunits identified specific protein bands of appropriate molecular mass (preadsorbed with the respective antigens) in specific tissues and cells that express nicotinic receptors, including the spinal cord and DRG neurons. Having established the absence of crossreactivity with related subunits, specific fluorescence labeling of nerve terminals and cell bodies was achieved and correlated with the distribution of defined marker proteins and nicotinic receptor binding sites determined autoradiographically. Our findings indicate that alpha3, alpha4, alpha5, beta2, and beta4 subunits are all expressed on primary afferents (IB4-positive terminals) in the spinal cord. The predominant presynaptic (synaptophysin colocalization) labeling is in the superficial layer of the dorsal horn. These receptor subunits, except for beta4, are also present in postsynaptic autonomic (anti-bNOS-positive) and somatic motor neurons (anti-VAChT-positive). The alpha3, alpha5, and beta2 subunits showed additional staining in glial (anti-GFAP-positive) cells. These studies reveal a dense and distinguishable distribution of nAChR subunits in the spinal cord and point toward future therapeutic targeting for specific spinal actions.

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Section: Colocalization Of Nicotinic Receptor Subunits With Cellular mentioning

confidence: 99%

Nicotinic acetylcholine receptor distribution in relation to spinal neurotransmission pathways

Khan

Osaka

Stanislaus

et al. 2003

J of Comparative Neurology

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“…Retrograde tracing from the corpora cavernosa has identified neurons in the spinal SPN and in the parvocellular region of the paraventricular nucleus and the medial preoptic area (Marson, 1999;Marson and Carson, 1999). Although the expression of the brain-type nNOS and NMDAR has been extensively characterized in the hypothalamus and spinal cord (Vernet et al, 1998;Uttenthal et al, 1998;Harada et al, 1999;Curras-Collazo et al, 2000;Herman et al, 2000;Gore, 2001;Reuss and Reuss, 2001;Sundstrom and Mo, 2001) and PIN has been found in some regions of the brain (Greenwood et al, 1997;Becker et al, 1999) and the spinal cord (Greenwood et al, 1997;Che et al, 2000), no data are available on the expression of PnNOS or NMDAR1-T in the CNS in general or of PIN in the hypothalamus.…”

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confidence: 99%

Penile neuronal nitric oxide synthase and its regulatory proteins are present in hypothalamic and spinal cord regions involved in the control of penile erection

Ferrini

Magee

Vernet

et al. 2003

J of Comparative Neurology

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Control of penile erection requires the coordination of the hypothalamus and the L6-S1 region of the spinal cord. Erection requires the activation of neuronal nitric oxide synthase (nNOS), which is tightly regulated. Because variants of nNOS (penile nNOS: PnNOS) and the N-methyl-D-aspartate receptor (truncated NMDAR subunit 1: NMDAR1-T) as well as protein inhibitor of NOS (PIN) have all been located in the pelvic ganglia and penile nerves, this work aims to determine whether these proteins are also present in the hypothalamus. It was found that PnNOS, the brain-type nNOS, and PIN, were expressed in the hypothalamus. In contrast, NMDAR1-T was expressed only in the penis, whereas the brain-type NMDAR1 was present in the brain and sacral spinal cord and not in the penis. PnNOS was found in the media preoptic area, posterior magnocellular, and the parvocellular regions of the paraventricular nucleus, supraoptic nucleus, septohypothalamic nucleus, medial septum, cortex, and in some of the nNOS staining neurons throughout the brain. It was absent in the organum vasculosum of the lamina terminalis. PIN staining was present in neurons of the medial preoptic area, paraventricular nucleus, medial septum, and cortex, but not in the supraoptic nucleus, septohypothalamic nucleus, or organum vasculosum of the lamina terminalis. Colocalization between PnNOS and PIN was found in the medial preoptic area, medial septum, and cortex, and less in the paraventricular nucleus. PnNOS and oxytocin were colocalized in the paraventricular nucleus and supraoptic nucleus. In hypothalamic extracts, recombinant PIN-GST protein bound to PnNOS in the extracts and partially inhibited NOS activity. These results indicate that both nNOS variants, and their respective regulatory proteins are present and colocalize in the hypothalamic and spinal cord regions involved in penile erection.

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“…The NR2B subunits are important candidates in the activation of NMDARs. 28,29 In the rats applied with ECS, the NMAD-NR2B receptor expression was up-regulated, which could be inhibited by propofol. 7 CaMK II is the main component of neuronal PSD, and > subunit of CaMK II is highly expressed in hippocampus and cerebral cortex.…”

Section: Discussionmentioning

confidence: 99%

Effects of Propofol on Expression of Hippocampal Neuronal Nitric Oxide Synthase and Carboxy-Terminal PDZ Ligand of Neuronal Nitric Oxide Synthase in Stressed Rats Undergoing Electroconvulsive Shock

Lv¹,

Shen²,

Peng³

et al. 2013

The Journal of ECT

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Nitric oxide synthase neurons in the rodent spinal cord: distribution, relation to Substance P fibers, and effects of dorsal rhizotomy

Cited by 37 publications

References 77 publications

Nicotinic acetylcholine receptor distribution in relation to spinal neurotransmission pathways

Nicotinic acetylcholine receptor distribution in relation to spinal neurotransmission pathways

Penile neuronal nitric oxide synthase and its regulatory proteins are present in hypothalamic and spinal cord regions involved in the control of penile erection

Effects of Propofol on Expression of Hippocampal Neuronal Nitric Oxide Synthase and Carboxy-Terminal PDZ Ligand of Neuronal Nitric Oxide Synthase in Stressed Rats Undergoing Electroconvulsive Shock

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