Nitric Oxide Synthase in Male Urological and Andrologic Functions

Yu, Qiuming; Li, Tieqiu; Li, Jingping; Zhong, Lintao; XiangmingMao,

doi:10.5772/67562

Cited by 8 publications

(3 citation statements)

References 133 publications

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“…These inflammatory mediators directly induce endothelial cell dysfunction and via nitric oxide pathway they may lead to male erectile dysfunction (Yu, Li, Li, Zhong, & Mao, 2017). Obesity is linked with several pathogenic conditions, such as diabetes, hypertension and dyslipidemia, which may be linked to the induction of ED (Shamloul & Ghanem, 2013).…”

Section: Obesity and Erectile Dysfunctionmentioning

confidence: 99%

Obesity and male infertility: Mechanisms and management

et al. 2020

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Obesity is characterised as an accumulation of excess body fat that has a detrimental impact on health outcomes and is clinically defined based on the body mass index (BMI), measured as weight (kg) divided by height (meters) squared (kg/m 2) (World Health Organization, 2000). The BMI is recommended by the World Health Organization (WHO) to clinically classify weight and determine clinical risk (Table 1) (McPherson & Lane, 2015). Within this system, obesity is defined as having a BMI of >30 kg/m 2 , while morbid obesity is defined as having a BMI of >35 kg/m 2 (Okorodudu et al., 2010). However, the type and distribution of body fat accumulation are of clinical importance in the assessment of the risk of obesity-related complications (Alberti et al., 2009). In fact, BMI does not consider lean body mass and fat mass proportions, nor the distribution of detrimental visceral adiposity (white adipose tissue) and protective subcutaneous adipose tissue. BMI is therefore considered to have a low sensitivity to appropriately determining adiposity and may underestimate or overestimate the risk of obesity in a significant proportion of the population (Okorodudu et al., 2010). The use of body fat percentage provides insight into the lean and fat mass ratios, differentiated between genders. The accepted consensus for increased risk of adiposity complications is >25% in males and >30% in females (Adler, Cassidy, Fricke, & Bonduriansky, 2013).

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Section: Obesity and Erectile Dysfunctionmentioning

confidence: 99%

Obesity and male infertility: Mechanisms and management

et al. 2020

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“…In this study, the concentration of testicular glycogen was unaffected, indicating that the extract had no effect on glycogen synthesis in testicular cells. Meanwhile, nitric oxide synthase (NOS) activity has been reported in male reproductive organs [48]. Endothelial nitric oxide synthase is expressed in Sertoli and Leydig cells at various stages of spermatogenesis in testicular cells [49].…”

Section: Discussionmentioning

confidence: 99%

Experimental validation and molecular docking to explore the active components of cannabis in testicular function and sperm quality modulations in rats

Nwonuma

Nwatu

Mostafa‐Hedeab

et al. 2022

BMC Complement Med Ther

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Background Data available support that ninety percent of male infertility cases are due to low sperm counts. There is a scarcity of data on the medicinal effects of cannabis on fertility. This study evaluated testicular function and sperm quality modulation with cannabis in rats. Methodology Twenty-five male Wistar rats were randomly grouped into five: A, B, C, and D, each group have 5 rats. A (control): 0.2 ml 2% DMSO, B (vitamin C): 90 mg/kg body weight, C, D, and E were administered: 5 mg/kg, 10 mg/kg and 20 mg/kg body weight of ethanolic leaf extract of cannabis (ELEC) respectively. The rats were sacrificed 24 h after the last day of the 60 day oral administrations. Flavonoids were the predominant phytochemical present in the extract while quercetin, kemferol, silyman and gallic acid were identified. Results The results showed a significant improvement (p < 0.05) in sperm quality and a significant increase in the concentrations of follicle-stimulating hormone, luteinizing hormone, triglycerides, cholesterol, and total protein determination compared to the normal control. Similarly, there was a significant increase (p < 0.05) in the activities of acid phosphatase, alkaline phosphatase, and superoxide dismutase compared to the normal control. RAC-alpha serine/threonine-protein kinase (AKT1)-silymarin complexes (-8.30 kcal/mol) and androgen receptor (AR)-quercetin complexes (9.20 kcal/mol) had the highest affinity. Conclusion The antioxidant effects of the flavonoids in the ethanolic extract of cannabis may have protected testicular and sperm cells from oxidative damage. Biochemical processes and histopathological morphology were preserved by cannabis. The docking prediction suggests that the bioactive principle of cannabis may activate the androgenic receptors. The androgenic receptor modulation may be attributed to silymarin and quercetin.

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“…In the testes, NO regulates tunica albuginea contraction and relaxation processes which provide forces needed to transport sperm cells from the testes to the epididymis (Lee & Cheng, 2009). Although gradual elevation in local NO levels in penile tissues ultimately leads to erection, a significantly high concentration of the signaling molecule was reported to have a detrimental effect on male tissue such as testes (Yu et al., 2017). Increased NO and its reactive nitrogen species derivatives have been associated with low sperm quality, testicular dysfunction and reduced gonadotrophin production (Doshi et al., 2012).…”

Section: Discussionmentioning

confidence: 99%

Ocimum tenuiflorum mitigates iron‐induced testicular toxicity via modulation of redox imbalance, cholinergic and purinergic dysfunctions, and glucose metabolizing enzymes activities

et al. 2021

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Oxidative stress is a primary culprit in the pathophysiology of infertility conditions in males. This study investigated the effects of Ocimum tenuiflorum on redox imbalance, cholinergic and purinergic dysfunctions and glucose dysmetabolism in oxidative‐mediated testicular toxicity using in vitro, ex vivo and in silico models. Induction of oxidative testicular injury was carried out by incubating normal testicular tissue with 0.1 mM FeSO4 and treated by co‐incubating with different concentrations of O. tenuiflorum infusion for 30 min at 37°C. O. tenuiflorum displayed significant ferric reducing power activity while scavenging DPPH and hydroxyl (OH˙) free radicals in vitro. Oxidative testicular injury significantly reduced the glutathione level and superoxide dismutase and catalase activities with concomitant elevation of malondialdehyde and nitric oxide levels and acetylcholinesterase, ATPase, fructose‐1,6‐bisphosphatase and glycogen phosphorylase (GlyP) activities. Incubation with the infusion significantly reversed these levels and activities. The phytochemical constituent of the infusion was detected by gas chromatography–mass spectroscopy analysis and revealed favourable binding energies when docked with some of the studied proteins. These results suggest O. tenuiflorum exerts a protective effect against Fe2+ induced testicular toxicity via mitigation of redox imbalance while modulating metabolic dysfunctions linked to male infertility.

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Nitric Oxide Synthase in Male Urological and Andrologic Functions

Cited by 8 publications

References 133 publications

Obesity and male infertility: Mechanisms and management

Obesity and male infertility: Mechanisms and management

Experimental validation and molecular docking to explore the active components of cannabis in testicular function and sperm quality modulations in rats

Ocimum tenuiflorum mitigates iron‐induced testicular toxicity via modulation of redox imbalance, cholinergic and purinergic dysfunctions, and glucose metabolizing enzymes activities

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