Nitric oxide synthase�II in rat skeletal muscles

Punkt, Karla; Naupert, Andreas; Wellner, Maren; Asmussen, G; Schmidt, Christine E.; Buchwalow, Igor

doi:10.1007/s00418-002-0465-4

Cited by 28 publications

(20 citation statements)

References 28 publications

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“…The reason for the differential effect of CH on sternohyoid and diaphragm muscle endurance remains unclear, but, in light of the present findings with chronic NOS blockade (see below), fibre-specific differences in NOS expression and activity may have been a contributing factor [35][36][37]. NOS activity is highest in fast oxidative fibres, which are considerably more abundant in diaphragm than in sternohyoid muscle.…”

Section: Ch-induced Functional Remodellingmentioning

confidence: 77%

Chronic hypoxia increases rat diaphragm muscle endurance and sodium-potassium ATPase pump content

McMorrow

Fredsted

Carberry

et al. 2010

European Respiratory Journal

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The effects of chronic hypoxia (CH) on respiratory muscle are poorly understood. The aim of the present study was to examine the effects of CH on respiratory muscle structure and function, and to determine whether nitric oxide is implicated in respiratory muscle adaptation to CH.Male Wistar rats were exposed to CH for 1-6 weeks. Sternohyoid and diaphragm muscle contractile properties, muscle fibre type and size, the density of fibres expressing sarco/ endoplasmic reticulum calcium-ATPase (SERCA) 2 and sodium-potassium ATPase (Na + ,K + -ATPase) pump content were determined. Muscle succinate dehydrogenase (SDH) and reduced nicotinamide adenine dinucleotide phosphate (NADPH) dehydrogenase activities were also assessed. Acute and chronic blockade of nitric oxide synthase (NOS) was employed to determine whether or not NO is critically involved in functional remodelling in CH muscles. CH improved diaphragm, but not sternohyoid, fatigue tolerance in a time-dependent fashion. This adaptation was not attributable to increased SDH or NADPH dehydrogenase activities. The areal density of muscle fibres and relative area of fibres expressing SERCA2 were unchanged. Na + ,K + -ATPase pump content was significantly increased in CH diaphragm. Chronic NOS inhibition decreased diaphragm Na + ,K + -ATPase pump content and prevented CH-induced increase in muscle endurance. This study provides novel insight into the mechanisms involved in CH-induced muscle plasticity. The results may be of relevance to respiratory disorders characterised by CH, such as chronic obstructive pulmonary disease.KEYWORDS: Chronic obstructive pulmonary disease, fatigue, myosin heavy chain isoforms, nitric oxide synthase, sarco/endoplasmic reticulum calcium-ATPase 2 S keletal muscle has enormous capacity for remodelling, as evident in various physiological and pathophysiological settings. Chronic hypoxia (CH), a feature of respiratory disease, is known to affect skeletal muscle structure and function. Alterations include changes in capillarity [1,2], fibre size and distribution [3][4][5][6][7][8][9][10], oxidative capacity [5,6,11,12] and contractile performance [5,9,[13][14][15][16]. CH induces reflex hyperventilation. Thus respiratory muscles are unique in that they must increase their workload in the face of a reduction in oxygen availability, necessary for aerobic metabolism. Respiratory muscle remodelling is a feature of chronic obstructive pulmonary disease (COPD) [17][18][19][20][21][22][23][24][25][26][27], which may be the result of hypoxic adaptation. Surprisingly, there is a general paucity of information concerning the effects of CH on respiratory muscle structure and function despite the clinical relevance. Translational animal models permit examination of the effects of CH on skeletal muscle independent of other confounding factors that are present in disease. Furthermore, they permit a thorough exploration of the molecular mechanisms that underpin muscle adaptation. As such, the major aim of the present study was to conduct a compreh...

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Section: Ch-induced Functional Remodellingmentioning

confidence: 77%

Chronic hypoxia increases rat diaphragm muscle endurance and sodium-potassium ATPase pump content

McMorrow

Fredsted

Carberry

et al. 2010

European Respiratory Journal

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“…Nos2a is expressed in many adult organs, in particular in immune tissues such as spleen and major entries for pathogens such as gills, as well as skin and gut which was already reported in trout (Campos-Perez et al, 2000). In mammals, nos2 is also expressed constitutively in various cell types and tissues such as retina and skeletal muscles (Park et al, 1996;Punkt et al, 2002). Zebrafish nos2a expression is inducible in vitro and in vivo by proinflammatory molecules.…”

Section: Divergence Of the Two Zebrafish Nos2 Genesmentioning

confidence: 84%

Comparative analysis of zebrafish nos2a and nos2b genes

Lepiller

Franche

Solary

et al. 2009

Gene

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“…Up to now, the relationship of NOS isoforms to different fibre types of human skeletal muscles was not addressed. Reports on NOS localization in different fibres of rat muscles contain rather confused matters due to the intermingling of different fibre classification systems (see Punkt et al 2001Punkt et al , 2002. When considering the fibre type related effects, it must be awared that muscle fibres may be specified according to three different classification systems as slow and fast (physiological fibre types), or as I, IIA, IIX (IIB; myosin based ATPase fibre types) or as SO (slow-oxidative), FOG (fast-oxidative glycolytic) and FG (fast glycolytic; physiological-metabolic fibre types; summarized in Punkt et al 2004).…”

Section: Introductionmentioning

confidence: 98%

Nitric oxide synthase in human skeletal muscles related to defined fibre types

Punkt

Fritzsche

Stockmar³

et al. 2005

Histochem Cell Biol

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Skeletal muscle functions regulated by NO are now firmly established. However, the knowledge about the NO synthase (NOS) expression related to a defined fibre type in human skeletal muscles necessitates further clarification. To address this issue, we examined localization of NOS isoforms I, II and III, in human skeletal muscles employing immunocytochemical labeling with tyramide signal amplification complemented with enzyme histochemistry and Western blotting. The NOS immunoreactivity was related to fibre types of different classification systems: physiological classification into slow and fast, ATPase classification into I, IIA, IIAX, IIX, and physiological-metabolic classification into slow-oxidative (SO), fast-oxidative glycolytic (FOG) and fast-glycolytic (FG). We found a correlation of NOS I-III immunoreactivity to metabolic defined fibre types with strong expression in FOG fibres. This implies that NO as modulator of muscle function is involved in oxidative metabolism in connection with fast force development, which only occurs in FOG fibres. The NOS expression showed no correlation to ATPase fibre subtypes due to the metabolic heterogeneity of ATPase fibre types. Healthy and affected vastus medialis muscles after anterior cruciate ligament rupture revealed similar NOS expression level as shown by Western blotting with, however, different expression patterns related to the fibre types in affected muscles. This suggests an altered modulation of force development in the fibres of diseased muscles.

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Nitric oxide synthase�II in rat skeletal muscles

Cited by 28 publications

References 28 publications

Chronic hypoxia increases rat diaphragm muscle endurance and sodium-potassium ATPase pump content

Chronic hypoxia increases rat diaphragm muscle endurance and sodium-potassium ATPase pump content

Comparative analysis of zebrafish nos2a and nos2b genes

Nitric oxide synthase in human skeletal muscles related to defined fibre types

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