Nitric Oxide Synthase and Xanthine Oxidase Activities in the Spermatic Vein of Patients with Varicocele: A Potential Role for Nitric Oxide and Peroxynitrite in Sperm Dysfunction

Mitropoulos, Dionisios; Deliconstantinos, George; Zervas, Anastasios; Villiotou, Vassiliki; Dimopoulos, C; Stavrides, John C.

doi:10.1016/s0022-5347(01)65403-x

Cited by 90 publications

(61 citation statements)

References 33 publications

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“…53 These patients have increased ROS in serum, testes, and semen samples. 54 Increased NO has also been demonstrated in the spermatic veins of patients with varicocele, 55,56 which could lead to spermatozoal dysfunction. 57 Varicocelectomy has been shown to increase the concentrations of antioxidants such as superoxide dismutase, catalase, glutathione peroxidase, and vitamin C in seminal plasma and improve the sperm quality.…”

Section: Varicocele and Osmentioning

confidence: 99%

REVIEW ARTICLE: Clinical Relevance of Oxidative Stress in Male Factor Infertility: An Update

Agarwal

Makker

Sharma

2007

American J Rep Immunol

678

507

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Male factor has been considered a major contributory factor to infertility. Along with the conventional causes for male infertility such as varicocele, cryptorchidism, infections, obstructive lesions, cystic fibrosis, trauma, and tumors, a new, yet important cause has been identified: oxidative stress. Oxidative stress (OS) is a result of the imbalance between reactive oxygen species (ROS) and antioxidants in the body, which can lead to sperm damage, deformity and eventually male infertility. This involves peroxidative damage to sperm membrane and DNA fragmentation at both nuclear and mitochondrial levels. OS has been implicated as the major etiological factor leading to sperm DNA damage. OS‐induced DNA damage can lead to abnormalities in the offspring including childhood cancer and achondroplasia. In this article, we discuss the need of ROS in normal sperm physiology, the mechanism of production of ROS and its pathophysiology in relation to male reproductive system. The benefits of incorporating antioxidants in clinical and experimental settings have been enumerated. We also highlight the emerging concept of utilizing OS as a method of contraception and the potential problems associated with it.

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Section: Varicocele and Osmentioning

confidence: 99%

REVIEW ARTICLE: Clinical Relevance of Oxidative Stress in Male Factor Infertility: An Update

Agarwal

Makker

Sharma

2007

American J Rep Immunol

678

507

View full text Add to dashboard Cite

show abstract

“…Thus, one clinically important issue regarding varicocele is preoperative recognition of who will respond to surgical treatment. Several studies have shown that ROS production is enhanced in varicocele patients [6,7], and that oxidative stress is present in spermatic vein blood and seminal plasma [8][9][10]. Oxidative stress results from an imbalance between the production and reduction of ROS.…”

Section: Discussionmentioning

confidence: 99%

“…In varicocele patients, ROS production is enhanced [6,7], and oxidative stress is present in spermatic vein blood and seminal plasma [8][9][10]. Varicocele treatment can significantly improve sperm parameters, by decreasing ROS concentration and increasing antioxidant capacity, and these effects lead to improved sperm parameters finally [11].…”

Section: Introductionmentioning

confidence: 99%

Genetic polymorphisms in glutathione S-transferase T1 affect the surgical outcome of varicocelectomies in infertile patients

Ichioka¹,

Nagahama²,

Okubo³

et al. 2009

Asian J Androl

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Glutathione S-transferases (GSTs), superoxide dismutase 2 (SOD2) and NAD(P)H:quinone oxidoreductase 1 (NQO1) are anti-oxidant enzyme genes. Polymorphisms of GSTs, SOD2 and NQO1 have been reported to influence individual susceptibility to various diseases. In an earlier study, we obtained preliminary findings that a subset of glutathione S-transferase T1 (GSTT1)-wt patients with varicocele may exhibit good response to varicocelectomy. In this study, we extended the earlier study to determine the distribution of genotype of each gene in the infertile population and to evaluate whether polymorphism of these genes affects the results of surgical treatment of varicocele. We analyzed 72 infertile varicocele patients, 202 infertile patients without varicocele and 101 male controls. Genotypes of GSTs were determined by polymerase chain reaction (PCR). Genotyping of SOD2 and NQO1 was performed using the PCR-restriction fragment length polymorphism (PCR-RFLP) method. A significantly better response to varicocelectomy was found in patients with the GSTT1-wt genotype (63.2%) and NQO1-Ser/Ser genotype (80.0%) than in those with GSTT1-null genotype (35.3%) and NQO1-Pro/Pro or NQO1-Pro/Ser genotype (45.2%), respectively. The frequencies of glutathione S-transferase M1/T1, SOD2 and NQO1 genotypes did not differ significantly among the varicocele patients, idiopathic infertile patients and male controls. GSTT1 genotype is associated with improvement of semen parameters after varicocelectomy. As the number of patients with NQO1-Ser/Ser genotype was not sufficient to reach definite conclusions, the association of NQO1 genotype with varicocelectomy requires further investigation.

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“…On the one hand, enhanced free radical generation by the spermatozoa and/or precursor germ cells has been repeatedly suggested, 65,72,73 on the other, there is evidence to suggest that excess free radical generation may involve the spermatic vein itself. 74 The excess generation of free radicals by the spermatozoa may be an indirect consequence of impaired spermatogenesis/epididymal function resulting in the retention of excess residual cytoplasm. The presence of excess cytoplasm has been positively correlated with the generation of ROS by human spermatozoa, via mechanisms involving the facilitated supply NADPH to oxidases in the sperm plasma membrane.…”

Section: Disruption Of the Antioxidant Status Of The Testesmentioning

confidence: 99%

Effects of Resistance Exercise Training on Antioxidant Enzyme and GLUT 8 Expression in Testes of Type 2 Diabetic Rats

Ahn¹,

Park²,

Hwan³

2017

Exerc Sci

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Nitric Oxide Synthase and Xanthine Oxidase Activities in the Spermatic Vein of Patients with Varicocele: A Potential Role for Nitric Oxide and Peroxynitrite in Sperm Dysfunction

Cited by 90 publications

References 33 publications

REVIEW ARTICLE: Clinical Relevance of Oxidative Stress in Male Factor Infertility: An Update

REVIEW ARTICLE: Clinical Relevance of Oxidative Stress in Male Factor Infertility: An Update

Genetic polymorphisms in glutathione S-transferase T1 affect the surgical outcome of varicocelectomies in infertile patients

Effects of Resistance Exercise Training on Antioxidant Enzyme and GLUT 8 Expression in Testes of Type 2 Diabetic Rats

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