Nitric oxide synthase and tyrosine nitration in idiopathic asthenozoospermia: an immunohistochemical study

Salvolini, Eleonora; Buldreghini, Eddi; Lucarini, Guendalina; Vignini, Arianna; Primio, Roberto Di; Balercia, Giancarlo

doi:10.1016/j.fertnstert.2011.12.022

Cited by 37 publications

(38 citation statements)

References 32 publications

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“…Consistent with this, asthenozoospermic infertile men present significantly higher ONOO 2 concentrations than normospermic fertile donors, revealing a negative correlation between ONOO 2 concentration and sperm motility (Vignini et al, 2006). Also, it has been affirmed that the high levels of peroxynitrite observed in patients together with high NOS activity are involved in the pathogenesis of idiopathic asthenozoospermia (Salvolini et al, 2012). In vitro studies have shown an association between high levels of peroxynitrite and impaired sperm motility.…”

Section: Discussionmentioning

confidence: 54%

“…Although oxidative stress has been widely associated with impaired sperm function and male infertility (Gharagozloo and Aitken, 2011), nitrosative stress caused by excessive RNS production may also contribute to impaired sperm function (Khosravi et al, 2012;Salvolini et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

“…In asthenozoospermic infertile men, higher levels of ONOO 2 and nitration of the tyrosine residues have been found than in normospermic fertile men (Vignini et al, 2006;Salvolini et al, 2012). A negative correlation has been reported between ONOO 2 concentration and sperm morphology and motility (Vignini et al, 2006;Khosravi et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

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Peroxynitrite-mediated nitrosative stress decreases motility and mitochondrial membrane potential in human spermatozoa

Uribe

Boguen

Treulén

et al. 2014

MHR: Basic Science of Reproductive Medicine

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Nitrosative stress is produced by high levels of reactive nitrogen species (RNS). The RNS include peroxynitrite, a highly reactive free radical produced from a diffusion-controlled reaction between nitric oxide and superoxide anion. Peroxynitrite causes nitration and oxidation of lipids, proteins and DNA, and is thus considered an important pathogenic mechanism in various diseases. Although high levels of peroxynitrite are associated with astenozoospermia, few reports exist regarding the in vitro effect of high levels of this RNS on human sperm. The aim of this study was to evaluate the in vitro effect of nitrosative stress caused by peroxynitrite on the viability, motility and mitochondrial membrane potential of human spermatozoa. To do this, human spermatozoa from healthy donors were exposed in vitro to 3-morpholinosydnonimine (SIN-1), a molecule that generates peroxynitrite. Incubations were done at 378C for up to 4 h with SIN-1 concentrations between 0.2 and 1.0 mmol/l. Generation of peroxynitrite was confirmed using dihydrorhodamine 123 (DHR) by spectrophotometry and flow cytometry. Sperm viability was assessed by propidium iodide staining; sperm motility was analyzed by CASA, and the state of mitochondrial membrane potential (DCm) by JC-1 staining. Viability and DCm were measured by flow cytometry. The results showed an increase in DHR oxidation, demonstrating the generation of peroxynitrite through SIN-1. Peroxynitrite decreased progressive and total motility, as well as some sperm kinetic parameters. Mitochondrial membrane potential also decreased. These alterations occurred with no decrease in sperm viability. In conclusion, peroxynitriteinduced nitrosative stress impairs vital functions in the male gamete, possibly contributing to male infertility.

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Section: Discussionmentioning

confidence: 54%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Peroxynitrite-mediated nitrosative stress decreases motility and mitochondrial membrane potential in human spermatozoa

Uribe

Boguen

Treulén

et al. 2014

MHR: Basic Science of Reproductive Medicine

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show abstract

“…Expression of all three isoforms of NOS (eNOS, nNOS, and iNOS), the NO producing enzyme, on the sperm membrane has been reported. A positive correlation between NOS enzyme activity and sperm motility is also established as inhibition of NOS enzyme either by L‐NAME or methylene blue decrease sperm motility (Donnelly, Lewis, Thompson, & Chakravarthy, ; Lewis et al, ; Salvolini et al, ). Human seminal plasma has been reported for sperm motility inhibitory as well as NOS activity reducing efficacy (de Lamirande, Belles‐Isles, & Gagnon, ; Schaad, Zhang, Campana, & Schorderet‐Slatkine, ).…”

Section: Discussionmentioning

confidence: 99%

A novel epididymal quiescence factor inhibits sperm motility by modulating NOS activity and intracellular NO‐cGMP pathway

Ghosh

Mukherjee

Bhoumik

et al. 2018

Journal Cellular Physiology

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Mature and potentially motile spermatozoa stored in cauda epididymis in an inactive state for approximately 30 days; however, during ejaculation they regain motility. To understand the actual molecular mechanism of the sperm quiescence during caudal stay, a proteinaceous quiescence factor (QF) has been purified from caprine epididymal plasma to apparent homogeneity. In the present study complete purification, detailed characterization as well as mechanistic pathway of QF has been described. QF is purified to 215-fold with 45% activity recovery. It is a 59 kDa monomeric protein with isoelectric point 5.8 and optimally active at pH 7.5. Circular dichroism spectroscopy and atomic force microscopy study confirm its α-helical secondary structure and globular tertiary conformation. QF is a thermo-stable protein as higher temperature does not alter its helical structure. N-terminal amino acid sequencing and MALDI analysis of QF did not find 100% similarity with any available protein of the database, proved its novelty. QF at 2 μM dose inhibits sperm progressive forward motility within 10 min. This motility inhibitory activity of QF is mediated by reducing NOS enzyme activity and subsequently decreasing the intracellular NO and cGMP concentration. It does not modulate intracellular Ca and cAMP concentration. QF has no adverse effect on DNA integrity and morphology of spermatozoa. Motility inhibitory action of QF is reversible. Thus, the role of QF in maintaining energy saving quiescence state of mature cauda spermatozoa and its reactive nitrogen species reducing activity may lead to a new direction for storage of spermatozoa and idiopathic male infertility.

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“…As positive controls for antibody against p53, p73 and TRAIL A549 cells treated with asbestiform antigorite were used. As positive controls for antibody against iNOS, isolated asthenozoospermic cells (Salvolini et al, 2012) were used. For negative controls primary antibodies were replaced with non-immune serum.…”

Section: Immunohistochemical Evaluationsmentioning

confidence: 99%

Raw and thermally treated cement asbestos exerts different cytotoxicity effects on A549 cells in vitro

et al. 2015

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Nitric oxide synthase and tyrosine nitration in idiopathic asthenozoospermia: an immunohistochemical study

Cited by 37 publications

References 32 publications

Peroxynitrite-mediated nitrosative stress decreases motility and mitochondrial membrane potential in human spermatozoa

Peroxynitrite-mediated nitrosative stress decreases motility and mitochondrial membrane potential in human spermatozoa

A novel epididymal quiescence factor inhibits sperm motility by modulating NOS activity and intracellular NO‐cGMP pathway

Raw and thermally treated cement asbestos exerts different cytotoxicity effects on A549 cells in vitro

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