Nitric Oxide Sensors for Biological Applications

Iverson, Nicole M.; Hofferber, Eric M.; Stapleton, Joseph A.

doi:10.3390/chemosensors6010008

Cited by 34 publications

(33 citation statements)

References 106 publications

(151 reference statements)

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“…4b-visible and infrared range). The RSNO concentration in unirradiated skin was found to be 35.14 ± 6.32 µmol•L −1 , which is similar to other reports (Iverson et al 2018). Figure 4a shows that the irradiation of human skin at 290, 300, 305, 340 and 400 nm decreased the amount of RSNO formation, in comparison with baseline.…”

Section: Figsupporting

confidence: 89%

“…NO is an important molecule in the homeostasis of human skin and overall health and involved in important physiological pathways such as vasodilation and macrophage toxicity (Wright and Weller 2015;Weller 2016). NO has a short halflife in the body of 0.05-1 ms (Iverson et al 2018). Thus in human skin NO is stored in the form of more stable species such as NO 2 − , NO 3 − and RSNOs, usually referred to as NO x species (Mowbray et al 2009;Liu et al 2014).…”

Section: Discussionmentioning

confidence: 99%

“…The electrochemical sensor was immersed into aqueous solutions i-iv or skin slice immersed in water and the signal of free NO production was electrochemically monitored, in the dark and under irradiation with the monochromator light source, described in item 2.4. The electrochemical sensor detects only free NO, since the sensor has a negative charged coating that allows NO pass through the coating while blocking other molecules such as NO 2 − and NO 3 − (Iverson et al 2018). The NO sensor was inserted into the solutions (around 1 cm below the top of the quartz cuvette) and the light guide connected to the monochromator was positioned 90° relative to the NO sensor.…”

Section: Real-time Free No Release Analysesmentioning

confidence: 99%

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Photochemistry of nitric oxide and S-nitrosothiols in human skin

Pelegrino

Paganotti

Seabra

et al. 2020

Histochem Cell Biol

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Nitric oxide (NO) is related to a wide range of physiological processes such as vasodilation, macrophages cytotoxicity and wound healing. The human skin contains NO precursors (NO x). Those are mainly composed of nitrite (NO 2 −), nitrate (NO 3 −), and S-nitrosothiols (RSNOs) which forms a large NO store. These NO x stores in human skin can mobilize NO to blood stream upon ultraviolet (UV) light exposure. The main purpose of this study was to evaluate the most effective UV light wavelength to generate NO and compare it to each NO precursor in aqueous solution. In addition, the UV light might change the RSNO content on human skin. First, we irradiated pure aqueous solutions of NO 2 − and NO 3 − and mixtures of NO 2 − and glutathione and NO 3 − and S-nitrosoglutathione (GSNO) to identify the NO release profile from those species alone. In sequence, we evaluated the NO generation profile on human skin slices. Human skin was acquired from redundant plastic surgical samples and the NO and RSNO measurements were performed using a selective NO electrochemical sensor. The data showed that UV light could trigger the NO generation in skin with a peak at 280-285 nm (UVB range). We also observed a significant RSNO formation in irradiated human skin, with a peak at 320 nm (UV region) and at 700 nm (visible region). Pre-treatment of the human skin slice using NO 2 − and thiol (RSHs) scavengers confirmed the important role of these molecules in RSNO formation. These findings have important implications for clinical trials with potential for new therapies.

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Section: Figsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Section: Real-time Free No Release Analysesmentioning

confidence: 99%

See 1 more Smart Citation

Photochemistry of nitric oxide and S-nitrosothiols in human skin

Pelegrino

Paganotti

Seabra

et al. 2020

Histochem Cell Biol

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“…It is difficult to detect physiological NO concentration directly, which is usually obtained indirectly by detecting nitrate concentration ex vivo . In previous reports, the detected physiological NO concentration is about submicromolar level .…”

Section: Resultsmentioning

confidence: 99%

A built‐in surface‐enhanced Raman scattering‐active microneedle for sampling in vivo and surface‐enhanced Raman scattering detection ex vivo of NO

Han

Sun

Wang

et al. 2018

J Raman Spectroscopy

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For sampling in vivo and surface‐enhanced Raman scattering (SERS) detection ex vivo, a built‐in SERS‐active microneedle is fabricated by adsorbing gold nanoshells (GNSs; as built‐in SERS‐active materials) in a corroded groove on an acupuncture needle. 3,4‐diaminobenzene‐thiol adsorbed on the GNSs is used as a selective SERS probe to detect nitric oxide (NO). The SERS‐active microneedles take SERS probes into the tissues of living mice with minimal invasion to react with NO selectively and then they are pulled out for direct and rapid SERS detection without any pretreatment steps or tedious operations. The built‐in SERS‐active microneedle would become a versatile platform for sampling in vivo and SERS detection ex vivo, taking SERS beyond culture cells to living animals.

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“…Because of the harmful effects of nitrite and nitrate, developing reliable techniques for detecting nitrite and nitrate contamination in foods has attracted significant interest to prevent toxicity. Therefore, in this work, a µPAD was evaluated for nitrite and nitrate detection, based on the Griess method [38,39]. Nitrate-to-nitrite reduction was attained with zinc followed by derivatization with sulfanilamide and N-(1-naphthyl)-ethylenediamine, which resulted in a red-pink azo dye being formed.…”

Section: Introductionmentioning

confidence: 99%

One-Step Polylactic Acid Screen-Printing Microfluidic Paper-Based Analytical Device: Application for Simultaneous Detection of Nitrite and Nitrate in Food Samples

2019

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In this work, we report a one-step approach for fabricating screened-printed microfluidic paper-based analytical devices (μPADs) using polylactic acid as a new hydrophobic material. A polylactic acid solution was screen printed onto chromatography papers to create hydrophobic patterns for fluidic channels. The optimal polylactic acid concentration for successful device fabrication is 9% w/v. The μPADs were fabricated within 2 min and provided high reproducibility and stability. The utility of polylactic acid screen-printing was demonstrated for the simultaneous detection of nitrite and nitrate using colorimetric detection. Under optimized experimental conditions, the detection limits and the linear ranges, respectively, were 1.2 mg L−1 and 2–10 mg L−1 for nitrite and 3.6 mg L−1 and 10–50 mg L−1 for nitrate. The detection times for both ions were found to be within 12 min. The developed μPAD was applied for the simultaneous determination of these ions in food samples, and no significant differences in the analytical results were observed compared to those of the reference method. The polylactic acid screen-printing approach presented here provides a simple, rapid, and cost-effective alternative fabrication method for fabricating μPADs.

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Nitric Oxide Sensors for Biological Applications

Cited by 34 publications

References 106 publications

Photochemistry of nitric oxide and S-nitrosothiols in human skin

Photochemistry of nitric oxide and S-nitrosothiols in human skin

A built‐in surface‐enhanced Raman scattering‐active microneedle for sampling in vivo and surface‐enhanced Raman scattering detection ex vivo of NO

One-Step Polylactic Acid Screen-Printing Microfluidic Paper-Based Analytical Device: Application for Simultaneous Detection of Nitrite and Nitrate in Food Samples

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