Nitric Oxide-Releasing Tryptophan-Based Poly(ester urea)s Electrospun Composite Nanofiber Mats with Antibacterial and Antibiofilm Activities for Infected Wound Healing

Abstract

Bacterial biofilms on wounds can lead to ongoing inflammation and delayed reepithelialization, which brings a heavy burden to the medical systems. Nitric oxide based treatment has attracted attention because it is a promising strategy to eliminate biofilms and heal infected wounds. Herein, a series of tryptophan-based poly­(ester urea)­s with good biodegradation and biocompatibility were developed for the preparation of composite mats by electrospinning. Furthermore, the mats were grafted with a nitric oxide donor (nitrosoglutathione, GSNO) to provide one type of NO loading cargo. The mats were found to have a prolonged NO release profile for 408 h with a maximum release of 1.0 μmol/L, which had a significant effect on killing bacteria and destructing biofilms. The designed mats were demonstrated to promote the growth of cells, regulate inflammatory factors, and significantly improve collagen deposition in the wound, eventually accelerating wound-size reduction. Thus, the studies presented herein provide insights into the production of NO-releasing wound dressings and support the application of full-thickness wound healing.

Natural Deep Eutectic Solvent Extraction of Bioactive Pigments from Spirulina platensis and Electrospinning Ability Assessment

“…During the electrospinning process, several jets are continuously elongated towards the collector, the solvent of the polymeric jets is evaporated quickly, phase separation occurs, the jet solidifies, and nanofibers are formed [ 9 ]. More importantly, the electrospun nanofibers exhibit decreased fiber diameter when compared with the microfibers fabricated from some conventional spinning methods, such as melt spinning, wet spinning, dry spinning, and dry-wet spinning, thus resulting in notably increased specific surface area, which is highly needed in several medicinal applications [ 29 , 30 ].…”

Quercetin-Loaded Polycaprolactone-Polyvinylpyrrolidone Electrospun Membranes for Health Application: Design, Characterization, Modeling and Cytotoxicity Studies

“…Quercetin supply antibacterial property at osteogenic potential of magnesium-doped calcium silicate [ 49 ], important to avoid antibiotic administration and for successful bone regeneration. Nanofiber with nitric oxide-based treatment promotes wound healing by improve collagen deposition, cells growth and regulate inflammatory factors [ 50 ], while electrospun radially-oriented nanofiber mats together with herbal compound loaded hydrogel accelerated in full-thickness skin wound healing in diabetic disease [ 51 ]. Thus, in this prospective study, our research could be considered a preliminary proof of concept whose findings indicated the formation of a highly stable system with promising features for health applications.…”

Biomechanical Behaviors and Degradation Properties of Multilayered Polymer Scaffolds: The Phase Space Method for Bile Duct Design and Bioengineering

“…The electrospinning technique is one advanced method that allows the creation of microfibrous scaffolds with desired properties from various materials for use in tissue engineering, treatment of infected wounds, etc. [ 51 , 52 ], and it may be used for the controlled formation of multilayered scaffolds.…”