Nitric oxide‐releasing microparticles as a potent antimicrobial therapeutic against chronic rhinosinusitis bacterial isolates

Abuzeid, Waleed M.; Girish, Vallerinteavide Mavelli; Fastenberg, Judd H.; Draganski, Andrew; Lee, Andrew Y.; Nosanchuk, Joshua D.; Friedman, Joel M.

doi:10.1002/alr.22185

Cited by 15 publications

(33 citation statements)

References 46 publications

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“…A clinical strain of PA derived from a CRS patient and used in earlier work evaluating SNO‐MP (PA1) and a reference PA strain, ATCC 15692, were used in this study . PA1 demonstrated an antimicrobial resistance typical of PA species without an acquired resistance to fluoroquinolones.…”

Section: Resultsmentioning

confidence: 99%

“…This study was approved by the institutional review board of the Albert Einstein College of Medicine. PA was obtained from patients diagnosed with CRS as described and was termed PA1 . In brief, patients with CRS diagnosed according to the updated adult clinical guidelines for sinusitis were consented .…”

Section: Methodsmentioning

confidence: 99%

“…SNO‐MP was synthesized as described elsewhere . In brief, an organosilica sol‐gel monolith was formed containing many covalently attached nitrosated thiol groups.…”

Section: Methodsmentioning

confidence: 99%

“…Established biofilms were divided into 4 treatment groups: (1) negative control with untreated bacterial suspension; (2) B‐MP (10 mg/mL); (3) SNO‐MP (10 mg/mL); (4) MH with SNO‐MP (10 mg/mL); (5) MH with nitrite (10 mmol/L); and (6) MH with nitrite (10 mmol/L) combined with SNO‐MP (10 mg/mL). SNO‐MP, whether blank or active, was applied at a concentration of 10 mg/mL, based on previously published data demonstrating efficacy against planktonic bacteria at this concentration . Biofilms were treated as described earlier before evaluation with CLSM.…”

Section: Methodsmentioning

confidence: 99%

“…We hypothesize that MH will reduce nitrite in vitro, thereby generating NO in concentrations that are able to disrupt Pseudomonas aeruginosa (PA) bacterial biofilms. We also hypothesize that the combination of MH and nitrite will augment the anti‐biofilm capability of a previously described NO‐releasing microparticle (SNO‐MP) …”

mentioning

confidence: 98%

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Novel nitric oxide‒generating platform using manuka honey as an anti‐biofilm strategy in chronic rhinosinusitis

Yang

Mavelli

Nacharaju

et al. 2019

Int Forum Allergy Rhinol

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Background Bacterial biofilms are implicated in the pathogenesis of chronic rhinosinusitis. Nitric oxide (NO) is a key immune effector with potent antimicrobial effects, but a short half‐life limits achievement of therapeutic concentrations. We hypothesized that manuka honey (MH) could induce sustained reduction of nitrite to NO causing biofilm disruption and that this effect would be enhanced with the addition of a NO‐releasing microparticle. Methods Porous organosilica microparticles containing nitrosylated thiol groups were formulated (SNO‐MP). MH was combined with serial dilutions of nitrite. NO release was evaluated using a NO analyzer. The susceptibility of 2 strains of Pseudomonas aeruginosa biofilms to these NO‐releasing platforms was evaluated using confocal microscopy. Cell viability and biofilm volume were quantified. Statistical analysis was performed using the Mann‐Whitney U test with SPSS software. Results MH with nitrite generated a linear increase in NO formation. SNO‐MP induced a bolus release of NO within 5 minutes, followed by a sustained plateau phase. MH with nitrite combined with SNO‐MP enhanced NO release during the plateau phase. MH with nitrite reduced biofilm live cells and volume by 88.5% to 96.9% and 95.1% to 95.6%, respectively, vs control (p < 0.0001). SNO‐MP reduced live cells and volume by 61.0% to 98.5% and 74.7% to 85.7%, respectively, vs control (p < 0.0001). MH with nitrite combined with SNO‐MP nearly eradicated biofilm, with a 98.3% to 99.8% (log 1.8‐2.6) reduction in viability and a 91.4% to 97.7% decrease in volume (p < 0.0001 vs control). Conclusion A novel platform that generates NO using MH and nitrite produces a potent anti‐biofilm effect, which can be further enhanced with the addition of SNO‐MP.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…SNO‐MP was synthesized as described elsewhere . In brief, an organosilica sol‐gel monolith was formed containing many covalently attached nitrosated thiol groups.…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 98%

See 3 more Smart Citations

Novel nitric oxide‒generating platform using manuka honey as an anti‐biofilm strategy in chronic rhinosinusitis

Yang

Mavelli

Nacharaju

et al. 2019

Int Forum Allergy Rhinol

Self Cite

View full text Add to dashboard Cite

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Moving slowly but surely toward more precise rhinologic care

Hwang¹

2018

Int Forum Allergy Rhinol

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Future topical medications in chronic rhinosinusitis

Miyake

Bleier

2019

Int Forum Allergy Rhinol

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Background:Research has progressed rapidly in recent decades to be er understand the etiopathogenesis and management paradigms of chronic rhinosinusitis (CRS). Although oral antibiotics o en mitigate symptoms in acute CRS exacerbations, eradication of polymicrobial biofilms and multidrug-resistant bacteria remains a challenge. The goal of this review is to summarize and discuss the potential and pitfalls of topical medications in the treatment of CRS. Methods:A related literature review was performed using PubMed and Scopus, with only the English database included. Results:The main therapies were selected and separated in sections. Details regarding future topical treatments of CRS were summarized and discussed. Conclusion:The ease of access of the sinonasal mucosa positions CRS as a disease with high potential for local topical treatment. The ultimate adoption of topical agents will require continued expansion of our understanding of novel local targets in CRS as well as improved methods to deliver and retain the drug of interest at the site of activity. C 2019 ARS-AAOA, LLC.

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Nitric oxide‐releasing microparticles as a potent antimicrobial therapeutic against chronic rhinosinusitis bacterial isolates

Abstract: SNO-MP demonstrates potent bactericidal effect against antibiotic-resistant CRS bacterial strains.

Cited by 15 publications

References 46 publications

Novel nitric oxide‒generating platform using manuka honey as an anti‐biofilm strategy in chronic rhinosinusitis

Novel nitric oxide‒generating platform using manuka honey as an anti‐biofilm strategy in chronic rhinosinusitis

Moving slowly but surely toward more precise rhinologic care

Future topical medications in chronic rhinosinusitis

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