Nitric Oxide Release from Porcine Aorta Endothelial Cells Measured by Electron Paramagnetic Resonance Spectroscopy

Fujii, Satoshi; Miyakoda, Goro; Chihiro, Masatoshi; Yoshimura, Tetsuhiko; Kamada, Hitoshi

doi:10.1246/cl.1996.1055

Cited by 8 publications

(14 citation statements)

References 10 publications

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“…No ESR spectral changes were observed with respect to the pattern and parameters due to NO-Fe(II)-DTCS in the circulating blood compared with the previous paper. [10] The g ' -and g // -values were found to be 2.04 and 2.02, respectively, with the ESR signals due to ceruloplasmin (g ¼ 2.06) and organic free radicals (g ¼ 2.01) (Fig. 3), as reported previously, [10] suggesting that NO-Fe(II)-DTCS existed stable in the circulating blood of normal rats.…”

Section: In Vivo Stability and Esr Parameters Of No-fe(ii)-dtcs In Thsupporting

confidence: 79%

“…1). [10,14] Therefore, nitrosyl-iron states in NO-Fe(II)-DTCS were stable in the biological fluids such as the blood, serum, bile, and urine under the experimental conditions. An isotropic ESR spectral pattern exhibited the existence of NO-Fe(II)-DTCS unbound to the erythrocytes, while the anisotropic spectral patterns in serum and RSA solution exhibited NO-Fe(II)-DTCS bound to serum proteins including RSA.…”

Section: Resultsmentioning

confidence: 92%

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Spinnokinetic Analyses of Blood Disposition and Biliary Excretion of Nitric Oxide (NO)-Fe(II)-N-(Dithiocarboxy)sarcosine Complex in Rats: BCM-ESR and BEM-ESR Studies

Yasui

Fujii

Yoshimura

2004

Free Radical Research

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Nitric oxide (NO) is well known to have a wide variety of biological and physiological functions in animals. On the basis of the fact that Fe(II)-dithiocarbamates react with NO, a Fe(II)-N-(dithiocarboxy)sarcosine complex (Fe(II)-DTCS) was proposed as a trapping agent for endogenous NO. However, quantitative pharmacokinetic investigation for NO-Fe(II)-dithiocarbamate complexes in experimental animals has been quite limited. This paper describes the results on the quantitative pharmacokinetic features of a NO-Fe(II)-N-DTCS in both the blood and bile of rats following intravenous (i.v.) administration of the complex. For this purpose, we applied two in vivo methods, i.e. (1) in vivo blood circulation monitoring-electron spin resonance (BCM-ESR) which previously developed, and (2) in vivo biliary excretion monitoring-electron spin resonance (BEM-ESR). We monitored real-time ESR signals due to nitrosyl-iron species in the circulating blood and bile flow. The ESR signal due to NO-Fe(II)-DTCS was stable in biological systems such as the fresh blood and bile. In in vivo BCM- and BEM-ESR, the pharmacokinetic parameters were calculated on the basis of the two-compartment and hepatobiliary transport models. The studies also revealed that the compound is widely distributed in the peripheral organs and partially excreted into the bile. We named a kinetic method to follow spin concentrations as spinnokinetics and this method will be useful for detecting and quantifying the endogenously generated NO in Fe(II)-DTCS administered animals.

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Section: In Vivo Stability and Esr Parameters Of No-fe(ii)-dtcs In Thsupporting

confidence: 79%

Section: Resultsmentioning

confidence: 92%

Spinnokinetic Analyses of Blood Disposition and Biliary Excretion of Nitric Oxide (NO)-Fe(II)-N-(Dithiocarboxy)sarcosine Complex in Rats: BCM-ESR and BEM-ESR Studies

Yasui

Fujii

Yoshimura

2004

Free Radical Research

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“…By use of the Fe-DTCS complex synthesized by Satoshi Fujii and colleagues [94,95], Yoshimura and colleagues could trap, stabilize and accumulate the nitrosylated ternary complex detected at 700 MHz, in the abdomen, particularly the liver, of LPS-treated mice and make a tomographical description of three-dimensional images [96]. The method was fully described and generalized with other spin-traps [116,117].…”

Section: Three-dimensional Epr Imaging and Other Recent Methodologicamentioning

confidence: 99%

“…Yoshimura, Fujii and others have developed dithiocarbamate derivatives expected to be even more water-soluble than MGD, such as N-(dithiocarboxy)sarcosine (DTCS) or having substituted proline, pyrrolidine and serine moiety such as N-methyl-L-serine and L-proline-dithiocarbamate [94][95][96][97][98]. These spin-traps are very efficient, close to the stoichiometry, and bind NO with large rate constant, 1.1 × 10 8 M -1 s -1 , which represents a real advantage for selectivity and time resolution over the much slower reaction of NO with CPTIO of 10 4 M -1 s -1 [70,97].…”

Section: Other Related Iron-dithiocarbamate Complexesmentioning

confidence: 99%

Contribution of spin-trapping EPR techniques for the measurement of NO production in biological systems

Henry¹,

Guissani²

2000

Analusis

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In this overview of NO spin-trapping methods, we have pointed out their main advantages over frozen solution EPR spectroscopy detection of nitrosylated metalloproteins: improved sensitivity, potential specificity and time-resolution. A few simple ideas concerning EPR methodology have been recalled. Examples of possible artefacts, sometimes unexpected, which can easily be encountered in any analytical methods have been described. New methodological developments have also been mentioned.

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“…The buffers employed in these experiments were 50 mmol/l sodium citrate-citric acid buffer (pH 5.0 and 6.0) and 50 mmol/l Tris-HCl buffer (pH 7.2 and 8.0). NO release from ISDN and GTN was also measured directly by an electron spin resonance (ESR) spectral method with a spin-trapping technique [17]. N-(Dithiocarboxy)sarcosine (DTCS)-Fe complex was used as NO trapping agent.…”

Section: No Liberation From Isdnmentioning

confidence: 99%

Effect of Isosorbide Dinitrate on Nitric Oxide Synthase under Hypoxia

Jiang

Yoneyama²,

Furukawa³

et al. 2001

Pharmacology

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Nitric oxide synthase (NOS) catalyzes nitric oxide (NO) formation from L-arginine in the presence of molecular oxygen and NADPH. NO is involved in the regulation of microvasculature. Isosorbide dinitrate (ISDN) and glyceryl trinitrate (GTN) have been widely used as vasodilators to treat acute myocardial ischemia, their biological effects being due to the release of NO. In this investigation, the effects of ISDN and GTN on NOS activity in the presence or absence of oxyhemoglobin under hypoxia and normoxia were studied. The apparent K_m values for molecular oxygen were 21.6 ± 1.5 and 9.4 ± 1.3 µmol/l for nNOS and eNOS, respectively. ISDN liberated NO in a concentration- and pH-dependent manner, but no differences between hypoxia and normoxia were observed. The NO release from ISDN was also measured directly by an electron spin resonance spectral method with N-(dithiocarboxy)sarcosine-Fe complex as a NO-trapping agent. ISDN increased nNOS and eNOS activities in the presence of 30 µmol/l oxyhemoglobin under hypoxia, while it did not affect nNOS and eNOS activities under normoxia. In the absence of oxyhemoglobin, ISDN inhibited nNOS and eNOS activities under both hypoxic and normoxic experimental conditions. The rate of oxygen release from oxyhemoglobin under hypoxia was increased 3 times in the presence of 1 mmol/l ISDN. In contrast to ISDN, GTN could not release NO spontaneously, and it also did not affect nNOS and eNOS activities in the absence or presence of 30 µmol/l oxyhemoglobin under both hypoxic and normoxic conditions. These results indicated that the NO release from ISDN is different from that of GTN, and the increase of NOS activity by ISDN in the presence of oxyhemoglobin under hypoxia is ascribed to the increase in molecular oxygen concentration.

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Nitric Oxide Release from Porcine Aorta Endothelial Cells Measured by Electron Paramagnetic Resonance Spectroscopy

Cited by 8 publications

References 10 publications

Spinnokinetic Analyses of Blood Disposition and Biliary Excretion of Nitric Oxide (NO)-Fe(II)-N-(Dithiocarboxy)sarcosine Complex in Rats: BCM-ESR and BEM-ESR Studies

Spinnokinetic Analyses of Blood Disposition and Biliary Excretion of Nitric Oxide (NO)-Fe(II)-N-(Dithiocarboxy)sarcosine Complex in Rats: BCM-ESR and BEM-ESR Studies

Contribution of spin-trapping EPR techniques for the measurement of NO production in biological systems

Effect of Isosorbide Dinitrate on Nitric Oxide Synthase under Hypoxia

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