Nitric oxide regulation of superoxide and peroxynitrite-dependent lipid peroxidation. Formation of novel nitrogen-containing oxidized lipid derivatives

Rubbo, Homero; Radí, Rafael; Trujillo, Madia; Telleri, R; Kalyanaraman, Balaraman; Barnes, Stephen; Kirk, Marion; Freeman, Bruce Α.

doi:10.1016/s0021-9258(18)47160-8

Cited by 1,208 publications

(192 citation statements)

References 72 publications

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“…If NO & was produced in high enough concentrations equivalent to endogenous levels of SOD, mitochondria would be irreversibly injured because the O 2 & 2 would preferentially react with NO & rather than SOD to produce ONOO 2 , a highly reactive and potentially cytotoxic molecule (Ischiropoulos et al 1992). The stability of ONOO 2 allows it to readily diffuse through a cell and interact with many biological targets, causing DNA condensation and damage, inhibition of protein synthesis, lipid and protein oxidation, and protein nitration (Rubbo et al 1994;Kim et al 1995). Results from this study indicate an approximate 2-fold increase in the amount of 3-NT found in old rats compared with young.…”

Section: The Journal Of Histochemistry and Cytochemistrymentioning

confidence: 99%

Aging Results in Increased Autophagy of Mitochondria and Protein Nitration in Rat Hepatocytes Following Heat Stress

Oberley

Swanlund

Zhang

et al. 2008

J Histochem Cytochem.

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The natural breakdown of cells, tissues, and organ systems is a significant consequence of aging and is at least partially caused by a decreased ability to tolerate environmental stressors. Based on quantitative ultrastructural analysis using transmission electron microscopy and computer imaging, we show significant differences in hepatocyte morphology between young and old rats during a 48-hr recovery period following a 2-day heat stress protocol. Mitochondrial injury was greater overall in old compared with young rats. Autophagy was observed in both young and old rats, with autophagy greater overall in old compared with young hepatocytes. Lipid peroxidation and protein nitration were evaluated by localization and quantification of 4-hydroxy-2-nonenal (4-HNE)-modified protein adducts and 3-nitrotyrosine (3-NT) levels, respectively. Levels of 3-NT but not 4-HNE-protein adducts were significantly elevated in hepatocytes of old rats in comparison with young at 90 min after heat stress, suggesting a major role for reactive nitrogen species in the pathology observed at this time point. These results show a differential response of hepatocyte mitochondria to heat stress with aging, as well as greater levels of both autophagic and nitrative damage in old vs young hepatocytes. This manuscript contains online supplemental material at http://www.jhc.org. Please visit this article online to view these materials.

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Section: The Journal Of Histochemistry and Cytochemistrymentioning

confidence: 99%

Aging Results in Increased Autophagy of Mitochondria and Protein Nitration in Rat Hepatocytes Following Heat Stress

Oberley

Swanlund

Zhang

et al. 2008

J Histochem Cytochem.

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“…Provocative recent studies have revealed that . NO can also function as a chainbreaking antioxidant by reacting with peroxyl radicals, and appears kinetically to be more effective in this than ␣-TOH, at least in model systems (52,53). In the process, lipid nitrites and nitrates appear to be generated via rearrangement of unstable nitrosoperoxyl intermediates (53).…”

Section: Enzymatic and Non-enzymatic Inhibitors Of Lipid Peroxidationmentioning

confidence: 99%

Lipid hydroperoxide generation, turnover, and effector action in biological systems

Girotti

1998

Journal of Lipid Research

1,006

198

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Lipid peroxidation is a well known example of oxidative damage in cell membranes, lipoproteins, and other lipid-containing structures. Peroxidative modification of unsaturated phospholipids, glycolipids, and cholesterol can occur in reactions triggered by i ) free radical species such as oxyl radicals, peroxyl radicals, and hydroxyl radicals derived from iron-mediated reduction of hydrogen peroxide or ii ) non-radical species such as singlet oxygen, ozone, and peroxynitrite generated by the reaction of superoxide with nitric oxide. Lipid hydroperoxides (LOOHs) are prominent non-radical intermediates of lipid peroxidation whose identification can often provide valuable mechanistic information, e.g., whether a primary reaction is mediated by singlet oxygen or oxyradicals. Cer tain cholesterolderived hydroperoxides (ChOOHs) have been used ver y effectively in this regard, both in model systems and cells. Being more polar than parent lipids, LOOHs perturb membrane structure/function and can be deleterious to cells on this basis alone. However, LOOHs can also participate in redox reactions, the nature and magnitude of which often determines whether peroxidative injury is exacerbated or prevented. Exacerbation may reflect iron-catalyzed one-electron reduction of LOOHs, resulting in free radicalmediated chain peroxidation, whereas prevention may reflect selenoperoxidase-catalyzed two-electron reduction of LOOHs to relatively non-toxic alcohols. LOOH partitioning between these two pathways in an oxidatively stressed cell is still poorly understood, but recent cell studies involving various ChOOHs have begun to shed light on this important question. An aspect of related interest that is under intensive investigation is lipid peroxidation/LOOHmediated stress signaling, which may evoke a variety of cellular responses, ranging from induction of antioxidant enzymes to apoptotic death. Ongoing exploration of these processes will have important bearing on our understanding of disease states associated with peroxidative stress.-Girotti, A. W. Lipid hydroperoxide generation, turnover, and effector action in biological systems.

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“…When the concentration of NO is greater than 1 µM, these intermediate species become significantly important because, at these concentrations, reactive nitrogen species (RNOS) like S-nitrosothiols (RSNO), peroxynitrite (OONO−), nitrogen dioxide (NO 2 ) are produced [1,47,48]. Peroxynitrite and nitrogen dioxide have demonstrated NO-associated lipid damage, which shows the antimicrobial activities associated with NO [1,49,50].…”

Section: Metal Oxide Nitric Oxide and Chitosan Npsmentioning

confidence: 99%

Pulling the Brakes on Fast and Furious Multiple Drug-Resistant (MDR) Bacteria

Khan

Manzoor

Sultan

et al. 2021

IJMS

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Life-threatening bacterial infections have been managed by antibiotics for years and have significantly improved the wellbeing and lifetime of humans. However, bacteria have always been one step ahead by inactivating the antimicrobial agent chemically or by producing certain enzymes. The alarming universal occurrence of multidrug-resistant (MDR) bacteria has compelled researchers to find alternative treatments for MDR infections. This is a menace where conventional chemotherapies are no longer promising, but several novel approaches could help. Our current review article discusses the novel approaches that can combat MDR bacteria: starting off with potential nanoparticles (NPs) that efficiently interact with microorganisms causing fatal changes in the morphology and structure of these cells; nanophotothermal therapy using inorganic NPs like AuNPs to destroy pathogenic bacterial cells; bacteriophage therapy against which bacteria develop less resistance; combination drugs that act on dissimilar targets in distinctive pathways; probiotics therapy by the secretion of antibacterial chemicals; blockage of quorum sensing signals stopping bacterial colonization, and vaccination against resistant bacterial strains along with virulence factors. All these techniques show us a promising future in the fight against MDR bacteria, which remains the greatest challenge in public health care.

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Nitric oxide regulation of superoxide and peroxynitrite-dependent lipid peroxidation. Formation of novel nitrogen-containing oxidized lipid derivatives

Cited by 1,208 publications

References 72 publications

Aging Results in Increased Autophagy of Mitochondria and Protein Nitration in Rat Hepatocytes Following Heat Stress

Aging Results in Increased Autophagy of Mitochondria and Protein Nitration in Rat Hepatocytes Following Heat Stress

Lipid hydroperoxide generation, turnover, and effector action in biological systems

Pulling the Brakes on Fast and Furious Multiple Drug-Resistant (MDR) Bacteria

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