Nitric oxide regulation of plant metabolism

Gupta, Kapuganti Jagadis; Kaladhar, Vemula Chandra; Fitzpatrick, Teresa B.; Fernie, Alisdair R.; Møller, Ian Max; Loake, Gary J.

doi:10.1016/j.molp.2021.12.012

Cited by 80 publications

(54 citation statements)

References 144 publications

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“…Since AOX reduces both ROS and RNS, it can have a positive effect on plant performance. The ONOO À produced under NaCl stress (Figure 4) can negatively affect many proteins via tyrosine nitration, which can irreversibly inactivate the proteins (Gupta et al, 2021). By regulating ONOO À , the AOX can improve plant metabolic activity under stress.…”

Section: Discussionmentioning

confidence: 99%

“…In recent years, nitric oxide (NO) emerged as an important signaling molecule, proven to play a wide array of roles in different physiological and developmental processes such as germination, root growth, respiration, stomatal closure, senescence and adaptive responses to several biotic and abiotic stresses (Kolbert et al, 2019; Neill et al, 2008). Several oxidative and reductive pathways operate and generate NO in various compartments of the cell (Gupta et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

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Alternative oxidase plays a role in minimizing ROS and RNS produced under salinity stress in Arabidopsis thaliana

Bhatoee

Singh

Kumar

et al. 2022

Physiologia Plantarum

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Under stress conditions, the overproduction of different reactive oxygen species (ROS) and reactive nitrogen species (RNS) causes imbalance in the redox homeostasis of the cell leading to nitro-oxidative stress in plants. Alternative oxidase (AOX) is a conserving terminal oxidase of the mitochondrial electron transport chain, which can minimize the ROS. Still, the role of AOX in the regulation of RNS during nitro-oxidative stress imposed by salinity stress is not known. Here, we investigated the role of AOX in minimizing ROS and RNS induced by 150 mM NaCl in Arabidopsis using transgenic plants overexpressing (AOX OE) and antisense lines (AOX AS) of AOX. Imposing NaCl treatment leads to a 4-fold enhanced expression of AOX accompanied by enhanced AOX capacity in WT Col-0. Further AOX-OE seedlings displayed enhanced growth compared with the AOX-AS line under stress. Examination of NO levels by DAF-FM fluorescence and chemiluminescence revealed thatAOX overexpression leads to reduced levels of NO. The total NR activity was elevated under NaCl, but no significant change was observed in wild-type (WT), AOX OE, and AS lines. The total ROS, superoxide, H 2 O 2 levels, and lipid peroxidation were higher in the AOX-AS line than in WT and AOX-OE lines. The peroxynitrite levels were also higher in the AOX-AS line than in WT and AOX-OE lines; further, the expression of antioxidant genes was elevated in AOX-AS. Taken together, our results suggest that AOX plays an important role in the mitigation of ROS and RNS levels and enhances plant growth, thus providing tolerance against nitro-oxidative stress exerted by NaCl.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Alternative oxidase plays a role in minimizing ROS and RNS produced under salinity stress in Arabidopsis thaliana

Bhatoee

Singh

Kumar

et al. 2022

Physiologia Plantarum

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“…Recently, different types of molecules previously considered toxic to cells have been found to exert signaling functions either directly or indirectly. Accordingly, molecules such as hydrogen peroxide (H 2 O 2 ) and nitric oxide (NO), which are part of the metabolism of reactive oxygen species (ROS) and reactive nitrogen species (RNS), have also been shown to be regulators of plant cellular metabolism, participating in all stages of plant development including seed germination, root and plant development, stomatal movement, senescence, flowering, and fruit ripening, as well as in the mechanisms of response to adverse environmental conditions ( Smirnoff and Arnaud, 2019 ; Liu et al , 2020 ; Rodrigues and Shan, 2021 ; Corpas et al , 2022 ; Gupta et al , 2022 ). Other molecules could also be placed in the same category, such as hydrogen sulfide (H 2 S), which has recently been shown to exert regulatory functions in numerous processes, including fruit ripening ( Gotor et al , 2019 ; Corpas, 2019 ; Corpas et al , 2021 ; Mishra et al , 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Interactions of melatonin, reactive oxygen species, and nitric oxide during fruit ripening: an update and prospective view

Corpas

Rodríguez-Ruiz

Muñoz‐Vargas

et al. 2022

Journal of Experimental Botany

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Fruit ripening is a physiological process that involves a complex network of signaling molecules that act as switches to activate or deactivate certain metabolic pathways at different levels, not only regulating the gene and protein expression but also through post-translational modifications (PTMs) of the involved proteins. Ethylene is the distinctive molecule that regulates the ripening of fruits, thus allowing their classification as climacteric and non-climacteric, according to their dependence or not of this phytohormone, respectively. However, in recent years it has been found that other molecules with signaling potential also exert regulatory roles not only by individually, but also due to the interactions among them. This implies mutual and hierarchical regulations that sometimes make it difficult to identify the initial triggering event. Among these “new” molecules, hydrogen peroxide (H2O2), nitric oxide (NO), and melatonin are highlighted. This review provides a comprehensive outline of the relevance of these molecules in the fruit ripening process and the complex network of the known interactions among them.

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“…In recent years, the roles of NO within plant biology have continued to expand. This small, redox‐active molecule plays several important roles in seed germination, root development, flowering, senescence, fruit ripening, stomatal movement, oxygen homeostasis, and regulation of primary and secondary metabolism, in addition to triggering protection against multiple biotic/abiotic stresses (Gupta et al., 2022; Kolbert et al., 2019). The emergence of NO as an important signal in plants has led to development of precise methods to determine the levels and location of this key molecule within plant tissues (Vishwakarma et al., 2019).…”

Section: Introductionmentioning

confidence: 99%

Detection of Nitric Oxide from Chickpea Using DAF Fluorescence and Chemiluminescence Methods

et al. 2022

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The free radical nitric oxide (NO) has emerged as an important signal molecule in plants, due to its involvement in various plant growth, development, and stress responses. For elucidating the role of NO, it is very important to precisely determine, localize, and quantify NO levels. Due to a relatively short half‐life and its rapid, complex reactivity with other radicals, together with its capacity to diffuse from the source of production, the quantification of NO in whole plants, tissues, organelles, and extracts is notoriously difficult. Hence, it is essential to employ sensitive procedures for precise detection of NO. Currently available methods can fulfill many requirements to precisely determine NO, but each method has several advantages and pitfalls. In this article, we describe a detailed procedure for the measurement of NO by diaminofluorescein (DAF) in cell‐permeable forms (DAF‐FM‐DA). In this method, the tissues are immersed in DAF‐FM DA, leading to their diffusion from the plasma membrane to the inside of the cell, where intracellular esterases cleave the ester bonds, leading to DAF‐FM release. The resulting DAF‐FM reacts with intracellularly generated NO and forms highly fluorescent triazolofluorescein (DAF‐FMT), which can be localized and monitored by fluorescence or confocal microscopy, and can also be detected via fluorimetry and flow cytometry. DAF dyes are very popular as they are non‐invasive, relatively easy to handle, and commercially available. Another precise and very sensitive method is chemiluminescence detection of NO, where NO reacts with ozone (O3), leading to emission of a quantum of light from which NO can be calculated. Using chickpea seedlings, we describe in detail the measurement of NO using DAF‐FM‐DA and chemiluminescence methods. © 2022 Wiley Periodicals LLC. Basic Protocol 1: Measurement of nitric oxide from chickpea seedlings using DAF‐FM DA fluorescence with fluorescence and confocal microscopy Basic Protocol 2: Chemiluminescence detection of nitric oxide from chickpea seedlings

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Nitric oxide regulation of plant metabolism

Cited by 80 publications

References 144 publications

Alternative oxidase plays a role in minimizing ROS and RNS produced under salinity stress in Arabidopsis thaliana

Alternative oxidase plays a role in minimizing ROS and RNS produced under salinity stress in Arabidopsis thaliana

Interactions of melatonin, reactive oxygen species, and nitric oxide during fruit ripening: an update and prospective view

Detection of Nitric Oxide from Chickpea Using DAF Fluorescence and Chemiluminescence Methods

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