Nitric oxide regulated cyclic di-GMP signaling

Boon, Elizabeth M.; Dai, Zhou; Liu, Niu; Henares, Bernadette M.; Arora, Dhruv P.; Lahiri, Tanaya

doi:10.1186/2050-6511-14-s1-o37

Cited by 3 publications

(2 citation statements)

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“…-LuxI: enzyme that synthesizes auto-inducing molecules, such as acyl-homoserine lactones (AHLs). -LuxS: enzyme involved in the synthesis of signaling molecules, such as autoinducer-2 (AI-2), which participates in intercellular communication [125,[135][136][137][138][139]…”

Section: Lux Regulatorsmentioning

confidence: 99%

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Relevance of the Adjuvant Effect between Cellular Homeostasis and Resistance to Antibiotics in Gram-Negative Bacteria with Pathogenic Capacity: A Study of Klebsiella pneumoniae

Rivera-Galindo,

Aguirre-Garrido,

Garza-Ramos

et al. 2024

Antibiotics

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Antibiotic resistance has become a global issue. The most significant risk is the acquisition of these mechanisms by pathogenic bacteria, which can have a severe clinical impact and pose a public health risk. This problem assumes that bacterial fitness is a constant phenomenon and should be approached from an evolutionary perspective to develop the most appropriate and effective strategies to contain the emergence of strains with pathogenic potential. Resistance mechanisms can be understood as adaptive processes to stressful conditions. This review examines the relevance of homeostatic regulatory mechanisms in antimicrobial resistance mechanisms. We focus on the interactions in the cellular physiology of pathogenic bacteria, particularly Gram-negative bacteria, and specifically Klebsiella pneumoniae. From a clinical research perspective, understanding these interactions is crucial for comprehensively understanding the phenomenon of resistance and developing more effective drugs and treatments to limit or attenuate bacterial sepsis, since the most conserved adjuvant phenomena in bacterial physiology has turned out to be more optimized and, therefore, more susceptible to alterations due to pharmacological action.

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Section: Lux Regulatorsmentioning

confidence: 99%

“…Transcriptional regulator that belongs to the family of LuxR-type regulators, acting by suppressing the expression of fimbriae and biofilm formation, in addition to regulating the expression of genes related to virulence. [136,139]…”

Section: Sdiamentioning

confidence: 99%

Relevance of the Adjuvant Effect between Cellular Homeostasis and Resistance to Antibiotics in Gram-Negative Bacteria with Pathogenic Capacity: A Study of Klebsiella pneumoniae

Rivera-Galindo,

Aguirre-Garrido,

Garza-Ramos

et al. 2024

Antibiotics

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Antibiotic failure: Beyond antimicrobial resistance

de la Fuente-Nunez,

Cesaro,

Hancock

2023

Drug Resistance Updates

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Functional metagenomic analysis of quorum sensing signaling in a nitrifying community

Tan

Yeo

Hafiz

et al. 2021

npj Biofilms Microbiomes

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Quorum sensing (QS) can function to shape the microbial community interactions, composition, and function. In wastewater treatment systems, acylated homoserine lactone (AHL)-based QS has been correlated with the conversion of floccular biomass into microbial granules, as well as EPS production and the nitrogen removal process. However, the role of QS in such complex communities is still not fully understood, including the QS-proficient taxa and the functional QS genes involved. To address these questions, we performed a metagenomic screen for AHL genes in an activated sludge microbial community from the Ulu Pandan wastewater treatment plant (WWTP) in Singapore followed by functional validation of luxI activity using AHL biosensors and LC–MSMS profiling. We identified 13 luxI and 30 luxR homologs from the activated sludge metagenome. Of those genes, two represented a cognate pair of luxIR genes belonging to a Nitrospira spp. and those genes were demonstrated to be functionally active. The LuxI homolog synthesized AHLs that were consistent with the dominant AHLs in the activated sludge system. Furthermore, the LuxR homolog was shown to bind to and induce expression of the luxI promoter, suggesting this represents an autoinduction feedback system, characteristic of QS circuits. Additionally, a second, active promoter was upstream of a gene encoding a protein with a GGDEF/EAL domain, commonly associated with modulating the intracellular concentration of the secondary messenger, c-di-GMP. Thus, the metagenomic approach used here was demonstrated to effectively identify functional QS genes and suggests that Nitrospira spp. maybe QS is active in the activated sludge community.

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Nitric oxide regulated cyclic di-GMP signaling

Cited by 3 publications

References 0 publications

Relevance of the Adjuvant Effect between Cellular Homeostasis and Resistance to Antibiotics in Gram-Negative Bacteria with Pathogenic Capacity: A Study of Klebsiella pneumoniae

Relevance of the Adjuvant Effect between Cellular Homeostasis and Resistance to Antibiotics in Gram-Negative Bacteria with Pathogenic Capacity: A Study of Klebsiella pneumoniae

Antibiotic failure: Beyond antimicrobial resistance

Functional metagenomic analysis of quorum sensing signaling in a nitrifying community

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