Nitric oxide production, systemic inflammation and lipid metabolism in periodontitis patients: possible gender aspect

Andrukhov, Oleh; Haririan, Hady; Bertl, Kristina; Rausch, Wolf‐Dieter; Bantleon, Hans-Peter; Moritz, Andreas; Rausch‐Fan, Xiaohui

doi:10.1111/jcpe.12145

Cited by 56 publications

(61 citation statements)

References 62 publications

(112 reference statements)

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“…Higher nitrite concentrations were found in whole saliva of periodontally healthy subjects in comparison with PD patients, and this situation remained unchanged after periodontal treatment [23]. In the present study, we demonstrated higher salivary NOx concentrations in the control group than in the periodontitis group, which is in agreement with other studies [23,24,25,45]. Conversely, some studies have observed the opposite; i.e., lower salivary NO levels in the healthy group in comparison with the periodontitis group [7,11,18,22].…”

Section: Discussionsupporting

confidence: 93%

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Inducible Nitric Oxide Synthase Polymorphisms and Nitric Oxide Levels in Individuals with Chronic Periodontitis

Scarel‐Caminaga

Flávia

Pigossi

et al. 2017

IJMS

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This study aimed to investigate whether the −1026(A>C)(rs2779249) and +2087(A>G)(2297518) polymorphisms in the NOS2 gene were associated with chronic periodontitis (CP) and with salivary levels of nitrite (NO2−) and/or nitrate + nitrite (NOx). A group of 113 mixed-race patients were subjected to periodontal, genetic, and biochemical evaluations (65 CP/48 periodontally healthy subjects). DNA was extracted from oral epithelial cells and used for genotyping by polymerase chain reaction (real-time). Salivary NOx concentrations were determined using an ozone-based chemiluminescence assay. Association of CP with alleles and genotypes of the −1026(A>C) polymorphism was found (X2 test, p = 0.0075; 0.0308), but this was not maintained after multiple logistic regression, performed to estimate the effect of covariates and polymorphisms in CP. This analysis demonstrated, after correction for multiple comparisons, that only the female gender was significantly associated with CP. Polymorphisms analyzed as haplotypes were not associated with CP. NOx levels were significantly higher in the control group of heterozygous individuals for both polymorphisms. In conclusion, the female gender was significantly associated with CP, and higher levels of salivary NOx were found in control subjects and associated with the heterozygous state of the NOS2 polymorphisms, reinforcing the potential of NO metabolites as markers of periodontitis status.

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Section: Discussionsupporting

confidence: 93%

“…Andrukhov et al, 2013 [45] observed that male patients with periodontitis exhibited significantly lower salivary NO 2 and serum NOx levels. This data suggests that gender might influence the NO production in periodontitis.…”

Section: Discussionmentioning

confidence: 99%

Inducible Nitric Oxide Synthase Polymorphisms and Nitric Oxide Levels in Individuals with Chronic Periodontitis

Scarel‐Caminaga

Flávia

Pigossi

et al. 2017

IJMS

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“…NO is produced from L -arginine by the enzyme NO synthase. It is an important intracellular and extracellular signaling molecule involved in diverse biological processes, including regulation of vascular tone, neurotransmission, and the immune response, and inhibition of platelet aggregation [56]. NO is also an important cytotoxic mediator under pathological conditions.…”

Section: Discussionmentioning

confidence: 99%

The anti-inflammatory and antioxidant effects of melatonin on LPS-stimulated bovine mammary epithelial cells

et al. 2017

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Mastitis is the most prevalent disease in dairy cattle worldwide and not only causes huge economic losses in the dairy industry but also threatens public health. To evaluate the therapeutic potential of melatonin in mastitis, we examined the ability of melatonin to protect bovine mammary epithelial cells (bMECs) from the harmful effects of lipopolysaccharide (LPS). We found that melatonin inhibited the LPS-binding protein–CD14–TLR4 signaling pathway in bMECs, which had opposing effects on pro-inflammatory and anti-inflammatory mediators. Melatonin decreased LPS-induced expression of pro-inflammatory cytokines, chemokines, and positive acute-phase proteins (APPs), including tumor necrosis factor-α, interleukin (IL)-1β, IL-6, granulocyte-monocyte colony-stimulating factor, chemokine CC motif ligand (CCL)2, CCL5, serum amyloid A, haptoglobin, C-reactive protein, ceruloplasmin, and α-1 antitrypsin, and increased expression of the anti-inflammatory cytokine IL-1Ra and the negative APP fibrinogen. In addition, melatonin increased dityrosine levels but suppressed nitrite levels by upregulating the expression of Nrf2 and heme oxygenase-1 in the Nrf2 antioxidant defense pathway. Finally, melatonin administration increased the viability of LPS-stimulated bMECs. These results suggest that melatonin protects bMECs from LPS-induced inflammatory and oxidant stress damage and provide evidence that melatonin might have therapeutic utility in mastitis.

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“…NO is produced from L-arginine by the enzyme NO synthase. It is an important intracellular and extracellular signaling molecule that is involved in diverse biological processes, including regulation of vascular tone, neurotransmission, immune response, and inhibition of platelet aggregation (Andrukhov et al, 2013). NO is also an important cytotoxic mediator under pathological conditions.…”

Section: Discussionmentioning

confidence: 99%

Protective Effect of Melatonin on LPS-stimulated Granulosa Cells in Japanese Quail

Isobe

Maeda

2017

J. Poult. Sci.

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The aim of this study was to evaluate the potential of melatonin to protect cultured granulosa cells from the harmful effects of lipopolysaccharide (LPS) in quail. Granulosa cells isolated from Japanese quails were pretreated with or without melatonin (10 or 100 μg/mL) for 12 h and then incubated for 12 h in the absence or presence of 100 ng/mL LPS. The expression of pro-inflammatory cytokines and chemokine was detected by quantitative real-time PCR. The levels of oxidative stress biomarkers (dityrosine and nitrite) were determined by ELISA and the Griess reaction. Cell viability was quantified using an MTT assay. Additionally, the level of progesterone was measured by ELISA. We found that melatonin decreased LPS-induced expression of IL-1β, . In addition, melatonin increased the dityrosine level, but suppressed the nitrite level. Finally, melatonin administration increased the viability of LPS-stimulated granulosa cells in vitro. However, progesterone basal secretion was not significantly changed. These results suggest that melatonin protects cultured granulosa cells from LPS-induced inflammatory and oxidative stress damage and provide evidence that melatonin might have therapeutic utility in ovarian follicle infection in Japanese quail.

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Nitric oxide production, systemic inflammation and lipid metabolism in periodontitis patients: possible gender aspect

Abstract: NO production is reduced in periodontitis, especially in male population. Gender might be an important factor in assessing risk of cardiovascular disease in periodontitis.

Cited by 56 publications

References 62 publications

Inducible Nitric Oxide Synthase Polymorphisms and Nitric Oxide Levels in Individuals with Chronic Periodontitis

Inducible Nitric Oxide Synthase Polymorphisms and Nitric Oxide Levels in Individuals with Chronic Periodontitis

The anti-inflammatory and antioxidant effects of melatonin on LPS-stimulated bovine mammary epithelial cells

Protective Effect of Melatonin on LPS-stimulated Granulosa Cells in Japanese Quail

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