Nitric oxide production in rectal dialysate is a marker of disease activity and location in children with inflammatory bowel disease

Canani, Roberto Berni; Cirillo, Paolo; Bruzzese, Eugenia; Graf, Maria; Terrin, Gianluca; Gaudiello, G.; Curtis, Mario De; Cucchiara, Salvatore; Guarino, Alfredo

doi:10.1111/j.1572-0241.2002.05757.x

Cited by 12 publications

(12 citation statements)

References 12 publications

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“…Rectal nitric oxide (NO) was measured in all patients by a dialysis bag, as described previously [22,23]. Briefly, a dialysis bag (3 cm long) was placed into the rectum and left for 30 min.…”

Section: Methodsmentioning

confidence: 99%

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Relationship between severe obesity and gut inflammation in children: what's next?

et al. 2010

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BackgroundPreliminary evidence suggests an association between obesity and gut inflammation.AimsTo evaluate the frequency of glucose abnormalities and their correlation with systemic and intestinal inflammation in severely obese children.Patients and MethodsThirty-four children (25 males; median age 10.8 ± 3.4 yrs) with severe obesity (BMI >95%) were screened for diabetes with oral glucose tolerance test (OGTT), systemic inflammation with C-reactive protein (CRP) and gut inflammation with rectal nitric oxide (NO) and faecal calprotectin.ResultsBMI ranged from 23 to 44 kg/m2, and BMI z-score between 2.08 e 4.93 (median 2.69 ± 0.53). Glucose abnormalities were documented in 71% of patients: type 2 diabetes in 29%, impaired fasting glucose (IFG) in 58%, and impaired glucose tolerance (IGT) in 37.5%. Thirty-one patients (91%) were hyperinsulinemic. CRP was increased in 73.5% with a correlation between BMI z-score and CRP (p 0.03). Faecal calprotectin was increased in 47% patients (mean 77 ± 68), and in 50% of children with abnormal glucose metabolism (mean 76 ± 68 μg/g), with a correlation with increasing BMI z-score. NO was pathological in 88%, and in 87.5% of glucose impairment (mean 6.8 ± 5 μM).ConclusionsIn this study, the prevalence of glucose abnormalities in obese children is higher than in other series; furthermore, a correlation is present between markers of systemic and intestinal inflammation and glucose abnormalities.

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Section: Methodsmentioning

confidence: 99%

“…NO and its stable metabolites NO2 - and NO3 - were measured by the Griess reaction. Normal value was considered < 5 uM [23]. …”

Section: Methodsmentioning

confidence: 99%

Relationship between severe obesity and gut inflammation in children: what's next?

et al. 2010

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“…So far, only H 2 and CH 4 have been shown to be measured reliably in such tests [21]. There are also some reports on gas analysis of the contents of rectally placed dialysis bags in humans [27].…”

Section: Intestinal Gases As Biomarkersmentioning

confidence: 98%

Human intestinal gas measurement systems: in vitro fermentation and gas capsules

Ou¹,

Yao²,

Rotbart³

et al. 2015

Trends in Biotechnology

106

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“…47,48 Luminal NO levels are also greatly increased in children with active IBD. 32,34,35 In healthy infants the levels of both cal protectin 49 and NO 50 can transiently become very high. This is probably part of the activation of the immune system during development of intestinal microflora and tolerance of such.…”

Section: Ibd Diagnosis In Childrenmentioning

confidence: 99%

“…with enema) are not needed (Table 1). Rectal NO levels are greatly increased in adults [30][31][32][33] and children 34,35 with ulcerative colitis or Crohn's disease, and the levels correlate with disease activity. In patients with clinically relapsed ulcerative colitis, NO levels decreased markedly after treatment with glucocorticoids, but only in patients who responded to treatment.…”

Section: Luminal Nitric Oxidementioning

confidence: 99%

Technology Insight: calprotectin, lactoferrin and nitric oxide as novel markers of inflammatory bowel disease

Lundberg

Hellström

Fagerhol

et al. 2005

Nat Rev Gastroenterol Hepatol

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Distinguishing patients with inflammatory bowel disease from those with irritable bowel syndrome can be difficult. A simple and reliable test that detects intestinal inflammation would therefore be very useful in the clinic. If such a test parameter correlated with the intensity of the inflammatory reaction it could also be used to monitor disease activity. Calprotectin, lactoferrin and nitric oxide are produced and released locally in much greater quantities in the inflamed gut than in the noninflamed gut. These compounds can be readily measured in fecal samples (calprotectin and lactoferrin) or directly in the intestinal lumen (nitric oxide gas). Here, we discuss what is known about these markers, how they could be used in clinical practice and how they can complement existing techniques used for the diagnosis and monitoring of inflammatory bowel disease.

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Nitric oxide production in rectal dialysate is a marker of disease activity and location in children with inflammatory bowel disease

Abstract: [No abstract available

Cited by 12 publications

References 12 publications

Relationship between severe obesity and gut inflammation in children: what's next?

Relationship between severe obesity and gut inflammation in children: what's next?

Human intestinal gas measurement systems: in vitro fermentation and gas capsules

Technology Insight: calprotectin, lactoferrin and nitric oxide as novel markers of inflammatory bowel disease

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