Nitric oxide production during the osteogenic differentiation of human periodontal ligament mesenchymal stem cells

Orciani, Monia; Trubiani, Oriana; Vignini, Arianna; Mattioli‐Belmonte, Monica; Primio, Roberto Di; Salvolini, Eleonora

doi:10.1016/j.acthis.2008.02.005

Cited by 42 publications

(38 citation statements)

References 36 publications

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“…In addition, noni leaf extract strongly increased matrix mineralization in MC3T3-E1 preosteoblasts. While ALP activity is a definitive early marker of osteoblast differentiation and activity in bone formation 28) , mineralized nodule formation is a marker of the late stage of osteoblast differentiation. Therefore, noni leaf extract was chosen over noni fruit aqueous extract to be the focus of investigation in this study for two reasons: it was capable of promoting differentiation and mineralization of both PDL and preosteoblast cell types as well as stimulating osteoblast differentiation and maturation.…”

Section: Discussionmentioning

confidence: 99%

“…The ability to grow and manipulate stem cells within the periodontal ligament is of considerable clinical significance, especially for developing novel mechanisms to achieve bone and periodontal tissue regeneration 28) . Previous findings have indicated that hPDL cells possess several osteoblast-like properties, including the ability to form mineralized nodules in vitro 13,29) when cultured in the presence of growth factors such as enamel matrix derivative (EMD) and transforming growth factor-beta1 (TGF-β1) 30) .…”

Section: Discussionmentioning

confidence: 99%

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Morinda citrifolia leaves enhance osteogenic differentiation and mineralization of human periodontal ligament cells

et al. 2014

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This present study investigated the potential of Morinda citrifolia leaf aqueous extract to induce osteogenic differentiation and matrix mineralization of human periodontal ligament (hPDL) cells. Human periodontal ligament cells were cultured in complete medium, ascorbic acid with β-glycerophosphate, or Morinda citrifolia leaf aqueous extract. Morinda citrifolia leaf aqueous extract significantly increased alkaline phosphatase activity compared to culturing in complete medium or ascorbic acid with β-glycerophosphate. Matrixcontaining mineralized nodules were formed only when the cells were cultured in the presence of Morinda citrifolia leaf aqueous extract. These nodules showed positive alizarin red S staining and were rich in calcium and phosphorus according to energy dispersive X-ray analysis. In conclusion, Morinda citrifolia leaf extract promoted osteogenic differentiation and matrix mineralization in human periodontal ligament cells, a clear indication of the therapeutic potential of Morinda citrifolia leaves in bone and periodontal tissue regeneration.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Morinda citrifolia leaves enhance osteogenic differentiation and mineralization of human periodontal ligament cells

et al. 2014

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“…The benefits of an NO donor in the context of bone formation shed new light on the treatment for osteoporosis. Furthermore, Orciani et al 13) reported the release of NO during osteogenic differentiation in human PDL cells, suggesting that local reimplantation of ex vivo expanded cells in conjugation with an NO donor could represent a promising approach for bone regenerative procedures.…”

Section: )mentioning

confidence: 99%

“…12) Recently, increased NO production during osteogenic differentiation has been reported in human PDL cells, 13) but the role of NO in osteoblastic differentiation of these cells requires further investigation.…”

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confidence: 99%

“…11) In addition, PDL cells can differentiate in response to a variety of extracellular stimuli, serving either to maintain homeostasis or to remodel, repair, and regenerate the surrounding hard tissue. 12) Recently, increased NO production during osteogenic differentiation has been reported in human PDL cells, 13) but the role of NO in osteoblastic differentiation of these cells requires further investigation.…”

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Nitric Oxide Modulates Osteoblastic Differentiation with Heme Oxygenase-1 via the Mitogen Activated Protein Kinase and Nuclear Factor-kappaB Pathways in Human Periodontal Ligament Cells

Lee

Choi

Yang

et al. 2009

Biological & Pharmaceutical Bulletin

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Nitric oxide (NO), synthesized from L-arginine by a family of constitutive and inducible NO synthases (iNOS), is a small, diffusible, diatomic, highly reactive molecule with a variety of cellular functions in all mammalian cells. 1) Recently, it was shown that NO plays an important role in stem cell proliferation and differentiation. It has been reported that, at physiological concentrations, NO functions as a negative regulator of stem/progenitor cell proliferation and is critical to the initiation of cell differentiation.2,3) NO also promotes bone and chondrocyte terminal differentiation ; stimulates preadipocyte differentiation in rat 6) ; and mediates osteoblastic differentiation.7) In addition, NO synthase inhibitors have been shown to arrest differentiation toward a cardiac phenotype in mouse embryonic stem cells; differentiation is rescued by NO donors. 8) In contrast, NO donor sodium nitroprusside (SNP) inhibits mineralization in the mouse chondrocyte-like ATDC5 cell line. 9) However, whether NO can affect the differentiation of periodontal ligament (PDL) cells is unknown.The PDL is a non-mineralized connective tissue which surrounds the tooth and exhibits osteoblast-like features, such as high alkaline phosphatase (ALP) activity and expression of osteonectin (ON), bone sialoprotein (BSP), osteocalcin (OC), and osteopontin (OPN). The cells that compose the PDL are capable of differentiating into osteoblasts or cementoblasts.10) Moreover, PDL cells have demonstrated stem cell properties such as self-renewal, clonogenicity, and multitissue differentiation potential.11) In addition, PDL cells can differentiate in response to a variety of extracellular stimuli, serving either to maintain homeostasis or to remodel, repair, and regenerate the surrounding hard tissue.12) Recently, increased NO production during osteogenic differentiation has been reported in human PDL cells, 13) but the role of NO in osteoblastic differentiation of these cells requires further investigation.Heme oxygenase (HO) is the rate-limiting enzyme involved in the catabolism of heme, yielding equimolar quantities of carbon monoxide (CO), free iron, and biliverdin; the latter two compounds are converted to ferritin and bilirubin, respectively. 14) One of three mammalian HO isoforms, HO-1 (also called heat shock protein 32), is a stress-responsive protein induced by various agents and is involved in a variety of regulatory and protective mechanisms in cells. 15,16) We previously reported that HO-1 is induced by pro-inflammatory cytokines, NO, substance P, hydrogen peroxide, and mechanical stress, [17][18][19][20][21][22][23] and that it may play a cytoprotective role in human pulp and PDL cells. However, HO-1-mediated NOinductive effects of osteoblastic differentiation in PDL cells has not yet been reported.Understanding the mechanisms that regulate osteogenic differentiation in human PDL cells will have important implications for the development of new therapeutic strategies in periodontal defects. This study aimed to investigate whether h...

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Expression of P2X7 ATP Receptor Mediating the IL8 and CCL20 Release in Human Periodontal Ligament Stem Cells

Trubiani

Horenstein

Caciagli

et al. 2014

J of Cellular Biochemistry

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ATP is released by human periodontal ligament cells (hPDLCs) and has been shown to regulate PDL regeneration and responses to mechanical stress through activation of P2Y receptors. This nucleotide, however, has also been reported to trigger the pro-inflammatory cascade by inducing the maturation and/or release of chemokines/cytokines from various cell types mainly via P2X7 receptors. Much less is known on the possible role of ATP in stem cells deriving from PDL (hPDLSCs) which are considered to be a promising tool for cell-based therapy to restore lesions. Given the role played by P2X7 in pathophysiological conditions, in this study we investigated the expression of P2X7 ATP receptors in hPDLSCs. The results obtained showed that hPDLSCs express P2X7 receptors evaluated by means of cytofluorimetric, immunohistochemistry, reverse transcriptase-PCR, and Western blot analyses. P2X7 ligation by 2',3'-(benzoyl-4-benzoyl)-ATP (BzATP), a specific receptor agonist, was followed by an increase in intracellular Ca2+ and in the uptake of ethidium bromide. These effects were dramatically reduced by oxidized ATP (oATP), the P2X7 irreversible inhibitor, suggesting that the P2X7 is the functional receptor involved. At 24 h treatment of hPDLSCs with BzATP it enhanced the release of the pro-inflammatory agents IL8 and CCL20, without influencing cell viability. These effects were counteracted by pre-treating the cells with oATP or with A-740003, a selective and potent P2X7 competitive antagonist. Collectively, these results indicated that extracellular ATP mediate a pro-inflammatory response via P2X7 receptors in hPDLSCs opening a further approach to control hPDLSCs behavior in their possible application as therapeutic tool.

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Nitric oxide production during the osteogenic differentiation of human periodontal ligament mesenchymal stem cells

Cited by 42 publications

References 36 publications

Morinda citrifolia leaves enhance osteogenic differentiation and mineralization of human periodontal ligament cells

Morinda citrifolia leaves enhance osteogenic differentiation and mineralization of human periodontal ligament cells

Nitric Oxide Modulates Osteoblastic Differentiation with Heme Oxygenase-1 via the Mitogen Activated Protein Kinase and Nuclear Factor-kappaB Pathways in Human Periodontal Ligament Cells

Expression of P2X7 ATP Receptor Mediating the IL8 and CCL20 Release in Human Periodontal Ligament Stem Cells

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