Nitric Oxide Production by the Human Intestinal Microbiota by Dissimilatory Nitrate Reduction to Ammonium

Vermeiren, Joan; Wiele, Tom Van de; Verstraete, Willy; Boeckx, Pascal; Boon, Nico

doi:10.1155/2009/284718

Cited by 50 publications

(40 citation statements)

References 59 publications

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“…11 and 12 it is evident that, both in wet and dry conditions, the sensors responses to benzene is considerably higher compared to that of interferers. These interferers were chosen because they are gasotransmitters (NO) [17] normally present in human gut, products of the bacterial activity in human stomach and intestine (NO x family [17][18][19]) or of highly reactive digestion processes (H 2 S). SO 2 was tested to verify the occurring of the chemical reaction…”

Section: Methodsmentioning

confidence: 99%

Detection of colorectal cancer biomarkers in the presence of interfering gases

Zonta

Anania

Fabbri

et al. 2015

Sensors and Actuators B: Chemical

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Medical studies have shown that tumor growth is accompanied by protein changes that may lead to the peroxidation of the cell membrane, with consequent emission of volatile organic compounds (VOCs). VOCs can be detected through breath or intestinal gases and are biomarkers for colorectal cancer (CRC). The analysis of VOCs represents a non-invasive and potentially inexpensive pre-screening technique. An array of chemoresistive gas sensors, based on screen-printed Metal OXide (MOX) semiconducting films, has been selected to identify gaseous compounds of oncological interest, i.e. benzene, 1-iodo-nonane and decanal, from the main interferers that can be found in the intestine. MOX sensors are able to detect concentrations down to about 10th ppb, as experimentally proven in previous works, so they can identify very slight differences in concentration among gas mixtures. In this work it has been proven that the array used is able to identify tumor markers singularly and in combination with other gases both in wet and dry conditions. Moreover, the sensors chosen can discriminate target VOCs from interferers even at low concentrations

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Section: Methodsmentioning

confidence: 99%

Detection of colorectal cancer biomarkers in the presence of interfering gases

Zonta

Anania

Fabbri

et al. 2015

Sensors and Actuators B: Chemical

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“…Enzyme bound NO and NH 2 OH are probable intermediates (Cruz-Garcia et al, 2007;Vermeiren et al, 2009) of DNRA. The ability to carry out DNRA is phylogenetically widespread.…”

Section: Dissimilatory Nitrate Reduction To Ammonium (Dnra)mentioning

confidence: 99%

Microbial nitrate respiration – Genes, enzymes and environmental distribution

Kraft

Strous

Tegetmeyer

2011

Journal of Biotechnology

357

252

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“…Activity of the large conductance Ca 2ϩ -activated K ϩ channel (BK channel) contributes to membrane potential and excitability of GI SMCs (19,58). The SMC BK channel is composed of an ␣-subunit and the SMC specific ␤ 1 -subunit.…”

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confidence: 99%

High-fat diet-induced obesity alters nitric oxide-mediated neuromuscular transmission and smooth muscle excitability in the mouse distal colon

Bhattarai

Fried

Gulbransen

et al. 2016

American Journal of Physiology-Gastrointestinal and Liver Physi

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-fat diet-induced obesity alters nitric oxide-mediated neuromuscular transmission and smooth muscle excitability in the mouse distal colon. Am J Physiol Gastrointest Liver Physiol 311: G210 -G220, 2016. First published June 10, 2016; doi:10.1152/ajpgi.00085.2016.-We tested the hypothesis that colonic enteric neurotransmission and smooth muscle cell (SMC) function are altered in mice fed a high-fat diet (HFD). We used wild-type (WT) mice and mice lacking the ␤1-subunit of the BK channel (BK␤1 Ϫ/Ϫ ). WT mice fed a HFD had increased myenteric plexus oxidative stress, a 28% decrease in nitrergic neurons, and a 20% decrease in basal nitric oxide (NO) levels. Circular muscle inhibitory junction potentials (IJPs) were reduced in HFD WT mice. The NO synthase inhibitor nitro-L-arginine (NLA) was less effective at inhibiting relaxations in HFD compared with control diet (CD) WT mice (11 vs. 37%, P Ͻ 0.05). SMCs from HFD WT mice had depolarized membrane potentials (Ϫ47 Ϯ 2 mV) and continuous action potential firing compared with CD WT mice (Ϫ53 Ϯ 2 mV, P Ͻ 0.05), which showed rhythmic firing. SMCs from HFD or CD fed BK␤1 Ϫ/Ϫ mice fired action potentials continuously. NLA depolarized membrane potential and caused continuous firing only in SMCs from CD WT mice. Sodium nitroprusside (NO donor) hyperpolarized membrane potential and changed continuous to rhythmic action potential firing in SMCs from HFD WT and BK␤1 Ϫ/Ϫ mice. Migrating motor complexes were disrupted in colons from BK␤1 Ϫ/Ϫ mice and HFD WT mice. BK channel ␣-subunit protein and ␤1-subunit mRNA expression were similar in CD and HFD WT mice. We conclude that HFD-induced obesity disrupts inhibitory neuromuscular transmission, SMC excitability, and colonic motility by promoting oxidative stress, loss of nitrergic neurons, and SMC BK channel dysfunction. enteric nervous system; large conductance calcium-activated K ϩ channel; gastrointestinal motility; obesity A HIGH-FAT DIET (HFD) can cause obesity (35) that leads to an increased risk for the development of type 2 diabetes (13,17,33), heart disease (29, 33), and arthritis (25). Obesity is also associated with gastrointestinal (GI) dysmotility (15,35,41,63). GI motility is controlled largely by interactions between enteric neurons, interstitial cells of Cajal (ICCs), and smooth muscle cells (SMCs) but how obesity alters the function of these cells is poorly understood.A HFD is associated with increased oxidative stress and altered function of myenteric neurons in rodent models of obesity (11,60,61). The neuropathological changes that cause GI dysmotility vary among animal models and location in the GI tract (11). Inhibitory motor neurons that express nitric oxide synthase (NOS) are most susceptible to damage caused by obesity (3, 10, 11). These neurons are crucial for coordination of SMC relaxation (23, 41). Thus the disruption of nitrergic neuron function is a mechanism that contributes to HFDinduced GI dysmotility.Enteric motorneurons regulate propulsive motility patterns while GI SMCs have rhythmic electrical activ...

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Nitric Oxide Production by the Human Intestinal Microbiota by Dissimilatory Nitrate Reduction to Ammonium

Cited by 50 publications

References 59 publications

Detection of colorectal cancer biomarkers in the presence of interfering gases

Detection of colorectal cancer biomarkers in the presence of interfering gases

Microbial nitrate respiration – Genes, enzymes and environmental distribution

High-fat diet-induced obesity alters nitric oxide-mediated neuromuscular transmission and smooth muscle excitability in the mouse distal colon

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