Nitric oxide (NO) synthase immunoreactivity in the starfish Marthasterias glacialis

Martı́nez, Alfredo; Riveros‐Moreno, Valentina; Polak, Julia M.; Moncada, Salvador; Sesma, P.

doi:10.1007/bf00318828

Cited by 62 publications

(18 citation statements)

References 27 publications

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“…The antiserum thus obtained has been shown to react immunocytochemically with neurons of different species (Springall et al, 1992;Terenghi et al, 1993;Martinez et al, 1994;Rodrigo et al, 1994) and to cross-react when used at low dilution (,1:1,000) with: pig, human and bovine endothelium (Springall et al, 1992). Western blots (antibody dilution 1:500) of different human tissue homogenates rich in neurons showed a protein band of 160 kDa, corresponding to neuronal NOS.…”

Section: Antibodymentioning

confidence: 96%

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Subcellular localization of nitric oxide synthase in the cerebral ventricular system, subfornical organ, area postrema, and blood vessels of the rat brain

et al. 1997

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The distribution of neuronal nitric oxide synthase (nNOS) has been studied in the more rostral portion of the lateral ventricle, subfornical organ, area postrema and blood vessels of the rat central nervous system. nNOS was located by means of a specific polyclonal antibody, by using light and electron microscopy. Light microscopy showed immunoreactive varicose nerve fibers and terminal boutons-like structures in the lateral ventricle, positioned in supra- and subependimal areas. The spatial relationships between immunoreactive neuronal processes and the wall of the intracerebral blood vessels were studied. Electron microscopy showed numerous nerve fibers in the wall of the lateral ventricle; many were nNos-immunoreactive and established very close contact with ependymal cells. Immunoreactive neurons and processes were found in the subependymal plate of the ventricular wall, the subfornical organ, the area postrema, and the circularis nucleus of the hypothalamus. In these last three areas, the immunoreactive neurons were found close to the perivascular space of fenestrated and nonfenestrated blood vessels. The nNOS immunoreactivity was localized to the endoplasmic reticulum, cisterns, ribosomes, neurotubules, and in the inner part of the external membrane. In the terminal boutons, the reaction product was found surrounding the vesicle membranes. This distribution showed nNOS as a predominantly membrane-bound protein. The nitrergic nerve fibers present in the wall of the ventricular system might regulate metabolic functions as well as neurotransmission in the subfornical organ, area postrema and circularis nucleus of the hypothalamus.

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Section: Antibodymentioning

confidence: 96%

“…It has also been described in invertebrate neural structures (Elphick et al, 1993;Martinez et al, 1994).…”

mentioning

confidence: 94%

Subcellular localization of nitric oxide synthase in the cerebral ventricular system, subfornical organ, area postrema, and blood vessels of the rat brain

et al. 1997

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“…NOS was purified from whole rat brain according to the method of Bredt and Snyder (1990) and injected into rabbits as described elsewhere (Springall et al, 1992;Riveros-Moreno et al, 1993). This antiserum has been shown to react immunocytochemically with neurons from different species (Springall et al, 1992;Terenghi et al, 1993;Martinez et al, 1994).…”

Section: Primary Antiseramentioning

confidence: 99%

Nitric oxide synthase in the Guinea pig preoptic area and hypothalamus: Distribution, effect of estrogen, and colocalization with progesterone receptor

Warembourg¹,

Leroy²,

Jolivet³

1999

J. Comp. Neurol.

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“…The aim of this study was the investigation on the oocytes of abyssal teleost Hoplostethus mediterraneus in order to ascertain the presence of bioactive molecules too [9][10][11][12][13] and to confirm their role in the reproductive function.…”

Section: Introductionmentioning

confidence: 99%

Histological, histochemical and immunohistochemical study on the growing oocytes of the abyssal teleost Hoplostethus mediterraneus (V).

Calabrò¹,

Albanese²,

Bertuccio³

et al. 2008

Folia Histochem Cytobiol.

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Abstract:The oocytes of the abyssal Teleost, Hoplostethus mediterraneus were studied. Four stages of growth were observed and the oocytes of all the stages were surrounded by follicular cells and had several nucleoli in the nucleus. In the oocytes of the II° stage, vacuoles without contents, in oocytes of the III° stage several vacuoles with a basophilic contents and small yolk globules were identified. General and basic proteins, ribonucleoproteins, acid proteoglycans with -COOH groups were recognized in the cytoplasm, in the nucleoli of oocytes in the II° stage and in the vacuolar contents of oocytes in the III° stage. In the follicular cells, in the pellucid zone, in the yolk globules, from their beginning, glycoproteins were present. Positivity, for all lectins used, was revealed in the follicular cells and in the four stages of oocytes growth. α-D-glucose and α-D-mannose binding sites were in the pellucid zone and in the initial yolk globules. In the lather galactose and β-N-acetyl glucosamine were present too. nNOS and VIP immunopositivity revealed at the periphery of the cytoplasm and at network of nerve fibres between oocytes, suggests NO is involved in a mechanism of regulation of the gametogenesis and of the spawning.

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Nitric oxide (NO) synthase immunoreactivity in the starfish Marthasterias glacialis

Cited by 62 publications

References 27 publications

Subcellular localization of nitric oxide synthase in the cerebral ventricular system, subfornical organ, area postrema, and blood vessels of the rat brain

Subcellular localization of nitric oxide synthase in the cerebral ventricular system, subfornical organ, area postrema, and blood vessels of the rat brain

Nitric oxide synthase in the Guinea pig preoptic area and hypothalamus: Distribution, effect of estrogen, and colocalization with progesterone receptor

Histological, histochemical and immunohistochemical study on the growing oocytes of the abyssal teleost Hoplostethus mediterraneus (V).

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