Nitric oxide modulates actin filament organization in <i>Arabidopsis thaliana</i> primary root cells at low temperatures

Plohovska, Svitlana H.; Krasylenko, Yuliya; Yemets, А. І.

doi:10.1002/cbin.10931

Cited by 21 publications

(11 citation statements)

References 51 publications

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“…Calcium (Ca 2+ ) serves as a versatile intracellular messenger that has been shown to regulate actin dynamics and rearrangements in plants [ 21 ]. Nitric oxide (NO) is a small bioactive signaling molecule that modulates actin filament organization in Arabidopsis primary root cells at low temperatures [ 22 ]. ROS have emerged as major regulatory molecules in plants and play roles in early signaling events initiated by environmental stimuli [ 23 ].…”

Section: Introductionmentioning

confidence: 99%

Actin filaments mediated root growth inhibition by changing their distribution under UV-B and hydrogen peroxide exposure in Arabidopsis

Wang²,

Chen

et al. 2020

Biol Res

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Background UV-B signaling in plants is mediated by UVR8, which interacts with transcriptional factors to induce root morphogenesis. However, research on the downstream molecules of UVR8 signaling in roots is still scarce. As a wide range of functional cytoskeletons, how actin filaments respond to UV-B-induced root morphogenesis has not been reported. The aim of this study was to investigate the effect of actin filaments on root morphogenesis under UV-B and hydrogen peroxide exposure in Arabidopsis. Results A Lifeact-Venus fusion protein was used to stain actin filaments in Arabidopsis. The results showed that UV-B inhibited hypocotyl and root elongation and caused an increase in H2O2 content only in the root but not in the hypocotyl. Additionally, the actin filaments in hypocotyls diffused under UV-B exposure but were gathered in a bundle under the control conditions in either Lifeact-Venus or uvr8 plants. Exogenous H2O2 inhibited root elongation in a dose-dependent manner. The actin filaments changed their distribution from filamentous to punctate in the root tips and mature regions at a lower concentration of H2O2 but aggregated into thick bundles with an abnormal orientation at H2O2 concentrations up to 2 mM. In the root elongation zone, the actin filament arrangement changed from lateral to longitudinal after exposure to H2O2. Actin filaments in the root tip and elongation zone were depolymerized into puncta under UV-B exposure, which showed the same tendency as the low-concentration treatments. The actin filaments were hardly filamentous in the maturation zone. The dynamics of actin filaments in the uvr8 group under UV-B exposure were close to those of the control group. Conclusions The results indicate that UV-B inhibited Arabidopsis hypocotyl elongation by reorganizing actin filaments from bundles to a loose arrangement, which was not related to H2O2. UV-B disrupted the dynamics of actin filaments by changing the H2O2 level in Arabidopsis roots. All these results provide an experimental basis for investigating the interaction of UV-B signaling with the cytoskeleton.

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Section: Introductionmentioning

confidence: 99%

Actin filaments mediated root growth inhibition by changing their distribution under UV-B and hydrogen peroxide exposure in Arabidopsis

Wang²,

Chen

et al. 2020

Biol Res

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“…The MTS of plants is closely related to cold‐resistance (Zhao et al., 2006). Micro‐tubulin stabilization modification can improve plant cold resistance (Blume et al., 2008; Plohovska et al., 2017); the relationship between TUA protein expression and plant cold resistance, however, is rarely reported. In this study, combining the results of phenotype observation, the overexpression of TUA protein under cold stress is correlated with cold resistance of plants.…”

Section: Discussionmentioning

confidence: 99%

Overexpression of sugarcane SoTUA gene enhances cold tolerance in transgenic sugarcane

et al. 2021

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Alpha (α)-tubulin (TUA) is a primary unit of cytoskeleton microtubules. In recent years, more frequent frosts have caused considerable yield loss in sugarcane (Saccharum spp. hybrid) production. Temperature is one of the most influential abiotic factors affecting the microtubule polymerization state in plants. In this research, we developed overexpressing SoTUA transgenic sugarcane lines from the cold-susceptible cultivar ROC22. The transgene was confirmed by polymerase chain reaction (PCR) and quantificational real-time polymerase chain reaction (qRT-PCR) analysis, while the relative expression of SoTUA protein was verified by Western blot. Compared with the nontransformed control plants (wild type [WT]), the transgenic lines showed higher relative protein expression than the WT at 25 ˚C, which indicates the overexpression of SoTUA protein. The contents of soluble protein and sugar and the activity of peroxidase (POD) were higher while the content of maleicdialdehyde was lower in the transgenic plants than the WT plants under the chilling treatment. Meanwhile, the expression levels of cold-defense related genes (P5CS, WRKY, Cu/Zn-SOD, and POD1) were higher in the transgenic lines than in the WT. These results suggest that the SoTUA protein plays an important role in protecting plants during chilling stress. This positive effect is not affected by transgenic plant generations.

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“…Under the action of arginase, arginine was decomposed into ornithine, and then ornithine was catalyzed by ornithine decarboxylase to synthesize putrescine, which has been shown to alleviate cold damage (Kovaćs et al, 2010;Zuo et al, 2021). Nitric oxide synthase is a key enzyme in arginine catabolism, and decomposes nitric oxide (NO) from arginine (Martıńez, 1995), while low temperature makes plants accumulate more NO to reduce cold damage (Plohovska et al, 2019). This also indicates that increased arginine accumulation is required to supply ornithine and NO production at low temperatures.…”

Section: B C a Figurementioning

confidence: 99%

Application of transcriptome analysis to investigate the effects of long-term low temperature stress on liver function in the tiger puffer (Takifugu rubripes)

et al. 2022

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The tiger puffer (Takifugu rubripes) is an important economic fish species in northern China. However, it is a warm-temperature species, and low winter temperatures can result in high mortality in aquaculture. Understanding the mechanisms of cold resistance in tiger puffers will thus provide critical information to help cope with winter cold. In this study, we performed transcriptome analysis of livers from puffer fish kept at different temperatures (18°C, 13°C, and 8°C) to identify the key pathways and genes involved in the response to low-temperature stress. We also detected serum levels of proteases, arginine, and proline to obtain further information on the response to cold adaption. Totals of 51, 942, and 195 differentially expressed genes were identified in the 18°C vs 13°C, 18°C vs 8°C, and 13°C vs 8°C groups, respectively. Pathway analysis showed that significantly enriched pathways were mainly related to digestion, metabolism, and environmental adaptation. Most genes in the pathways related to digestion and metabolism were down-regulated, while most genes in the pathways related to environmental adaptation were up-regulated. Serum levels of proteases were significantly lower in the low-temperature groups (13°C and 8°C) compared with the control group (18°C), while arginine and proline levels were significantly higher in the 8°C group compared with the other two groups. These results suggest that low temperature caused digestive and metabolic disorders, as well as adaptive changes to low temperature in tiger puffers. On this premise, we found that some up-regulated genes in the pancreatic secretion pathway, arginine and proline metabolism pathway, and circadian rhythm pathway played important roles in the survival, growth, and development of tiger puffers under low-temperature stress. The accumulation of arginine and proline can maintain metabolism and circulation and resist cold stress. The circadian rhythm is closely related to digestion and metabolism, which is an adaptive change and plays a positive role in the resistance to low temperature. The results of this study provide new insights and a theoretical basis for the study of cold tolerance in tiger puffers.

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Nitric oxide modulates actin filament organization in Arabidopsis thaliana primary root cells at low temperatures

Cited by 21 publications

References 51 publications

Actin filaments mediated root growth inhibition by changing their distribution under UV-B and hydrogen peroxide exposure in Arabidopsis

Actin filaments mediated root growth inhibition by changing their distribution under UV-B and hydrogen peroxide exposure in Arabidopsis

Overexpression of sugarcane SoTUA gene enhances cold tolerance in transgenic sugarcane

Application of transcriptome analysis to investigate the effects of long-term low temperature stress on liver function in the tiger puffer (Takifugu rubripes)

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