Nitric oxide mediates brassinosteroid-induced flavonoid biosynthesis in Camellia sinensis L.

Li, Xin; Zhang, Lan; Ahammed, Golam Jalal; Li, Zhixin; Wei, Ji‐Peng; Shen, Chen; Peng, Yan; Zhang, Liping; Wang, Han

doi:10.1016/j.jplph.2017.04.005

Cited by 79 publications

(42 citation statements)

References 20 publications

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“…The scavenger and inhibitors of NO not only inhibited the production of endogenous NO, but also inhibited the effect of the exogenous BR. This observation was consistent with the findings of Li et al [56]. Our results suggested that nitric oxide was involved in the brassinolide-induced adventitious root development in cucumber explants.…”

Section: Discussionsupporting

confidence: 94%

Nitric oxide is involved in the brassinolide-induced adventitious root development in cucumber

Liao

et al. 2020

BMC Plant Biol

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Background: Brassinolide (BR), as a new type of plant hormones, is involved in the processes of plant growth and stress response. Previous studies have reported the roles of BR in regulating plant developmental processes and also response tolerance to abiotic stresses in plants. The main purpose of our study was to explore whether nitric oxide (NO) plays a role in the process of BR-induced adventitious root formation in cucumber (Cucumis sativus L.).Results: Exogenous application of 1 μM BR significantly promoted adventitious rooting, while high concentrations of BR (2-8 μM) effectively inhibited adventitious rooting. NO donor (S-nitroso-N-acerylpenicillamine, SNAP) promoted the occurrence of adventitious roots. Simultaneously, BR and SNAP applied together significantly promoted adventitious rooting and the combined effect was superior to the application of BR or SNAP alone. Moreover, NO scavenger (c-PTIO) and inhibitors (L-NAME and Tungstate) inhibited the positive effects of BR on adventitious rooting. BR at 1 μM also increased endogenous NO content, NO synthase (NOS-like) and Nitrate reductase (NR) activities, while BRz (a specific BR biosynthesis inhibitor) decreased these effects. In addition, the relative expression level of NR was up-regulated by BR and SNAP, whereas BRz down-regulated it. The application of NO inhibitor (Tungstate) in BR also inhibited the up-regulation of NR.Conclusion: BR promoted the formation of adventitious roots by inducing the production of endogenous NO in cucumber.

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Section: Discussionsupporting

confidence: 94%

Nitric oxide is involved in the brassinolide-induced adventitious root development in cucumber

Liao

et al. 2020

BMC Plant Biol

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“…This observation was consistent with the findings of Li et al [56]. Our results suggested that nitric oxide was involved in the brassinolide-induced adventitious root development in cucumber explants.…”

Section: Discussionsupporting

confidence: 94%

Nitric oxide is involved in the brassinolide-induced adventitious root development in cucumber

Liao

et al. 2020

Preprint

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Background: Brassinolide (BR), as a new type of plant hormones, is involved in the processes of plant growth and stress response. Previous studies have reported the roles of BR in regulating plant developmental processes and also response tolerance to abiotic stresses in plants. The main purpose of our study was to explore whether nitric oxide (NO) plays a role in the process of BR-induced adventitious root formation in cucumber ( Cucumis sativus L.). Results: Exogenous application of 1 μM BR significantly promoted adventitious rooting, while high concentrations of BR (2-8 μM) effectively inhibited adventitious rooting. NO donor (S-nitroso-N-acerylpenicillamine, SNAP) promoted the occurrence of adventitious roots. Simultaneously, BR and SNAP applied together significantly promoted adventitious rooting and the combined effect was superior to the application of BR or SNAP alone. Moreover, NO scavenger (c-PTIO) and inhibitors (L-NAME and Tungstate) inhibited the positive effects of BR on adventitious rooting. BR at 1 μM also increased endogenous NO content, NO synthase (NOS-like) and Nitrate reductase (NR) activities, while BRz (a specific BR biosynthesis inhibitor) decreased these effects. In addition, the relative expression level of NR was up-regulated by BR and SNAP, whereas BRz down-regulated it. The application of NO inhibitor (Tungstate) in BR also inhibited the up-regulation of NR . Conclusion: BR promoted the formation of adventitious roots by inducing the production of endogenous NO in cucumber.

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“…Brassinosteroids are a group of phytohormones that regulate multiple physiological and metabolic processes in plants ( Ahammed et al, 2014 ; Li et al, 2016 , 2017a ). BRs also enhance tolerance to abiotic stress, including high temperature stress in a range of crop species ( Ahammed et al, 2014 , 2016 ).…”

Section: Introductionmentioning

confidence: 99%

Brassinosteroids Attenuate Moderate High Temperature-Caused Decline in Tea Quality by Enhancing Theanine Biosynthesis in Camellia sinensis L.

Wei

Ahammed

et al. 2018

Front. Plant Sci.

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Temperature is a major environmental signal that governs plant growth and development. A moderately high ambient temperature alters plant metabolism without significant induction of heat–stress responses. Despite ancillary reports on the negative effect of warmer climate on tea quality, information on specific effect of sub high temperature (SHT) on theanine accumulation is scanty. L-Theanine is the most abundant free amino acid in tea (Camellia sinensis L.) leaves that contributes to the unique umami flavor of green tea infusion. Tea harvested in warmer months lacks distinctive umami taste due to low theanine content. In this study, we showed that SHT (35°C) gradually decreased theanine concentration over time, which was closely associated with the SHT-induced suppression in theanine biosynthetic genes. 24-epibrassinolide (BR), a bioactive brassinosteroids, attenuated the SHT-induced reduction in theanine concentration by upregulating the transcript levels of theanine biosynthetic genes, such as ARGININE DECARBOXYLASE (CsADC), GLUTAMINE SYNTHETASE (CsGS), GLUTAMATE SYNTHASE (CsGOGAT) and THEANINE SYNTHASE (CsTS). Furthermore, time-course analysis of the activity of theanine biosynthetic enzyme reveals that BR-induced regulation of GS and GOGAT activity plays essential role in maintaining theanine content in tea leaves under SHT, which is consistent with the central position of GOGAT in theanine biosynthetic pathway. Therefore, it is convincing to propose that exogenous BR treatment can be advocated to improve summer tea quality by enhancing in vivo accumulation of theanine. However, a future challenge is to use this information on the role of BR in theanine biosynthesis and thermotolerance to further understand how BR may be tuned to benefit plant fitness for enhancing tea quality.

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Nitric oxide mediates brassinosteroid-induced flavonoid biosynthesis in Camellia sinensis L.

Cited by 79 publications

References 20 publications

Nitric oxide is involved in the brassinolide-induced adventitious root development in cucumber

Nitric oxide is involved in the brassinolide-induced adventitious root development in cucumber

Nitric oxide is involved in the brassinolide-induced adventitious root development in cucumber

Brassinosteroids Attenuate Moderate High Temperature-Caused Decline in Tea Quality by Enhancing Theanine Biosynthesis in Camellia sinensis L.

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