Nitric oxide measurements in biological samples

Taha, Ziad

doi:10.1016/s0039-9140(03)00354-0

Cited by 101 publications

(60 citation statements)

References 49 publications

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“…Development of sensitive and selective analytical techniques for the measurement of NO Å in biological systems has been a matter of great concern (reviewed in Packer, 2002 andTaha, 2003). The techniques so far implemented in biomedical research explore the physical and chemical properties that support NO Å biological activity and provide complementary information.…”

Section: Measurement Of No å In Hippocampal Subregions Using Electrocmentioning

confidence: 99%

Nitric oxide in brain: diffusion, targets and concentration dynamics in hippocampal subregions

Ledo

Frade

Barbosa

et al. 2004

Molecular Aspects of Medicine

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Nitric oxide (NO Å ) is a diffusible regulatory molecule involved in a wide range of physiological and pathological events. At the tissue level, a local and temporary increase in NO Å concentration is translated into a cellular signal. From our current knowledge of biological synthesis and decay, the kinetics and mechanisms that determine NO Å concentration dynamics in tissues are poorly understood. Generally, NO Å mediates its effects by stimulating (e.g., guanylate cyclase) or inhibiting (e.g., cytochrome oxidase) transition metal-containing proteins and by post-translational modification of proteins (e.g., formation of nitrosothiol adducts). The borderline between the physiological and pathological activities of NO Å is a matter of controversy, but tissue redox environment, supramolecular organization and compartmentalisation of NO Å targets are important features in determining NO Å actions. In brain, NO Å synthesis in the dependency of glutamate NMDA receptor is a paradigmatic example; the NMDA-subtype glutamate receptor triggers intracellular signalling pathways that govern neuronal plasticity, development, senescence and disease, suggesting a role for NO Å in these processes. Measurements of NO Å in the different subregions of hippocampus, in a glutamate NMDA receptor-dependent fashion, by means of electrochemical selective microsensors illustrate the concentration dynamics of NO Å in the sub-regions of this brain area. The analysis of NO Å concentration-time profiles in the hippocampus requires consideration of at least two interrelated issues, also addressed in this review. NO Å diffusion in a biological medium and regulation of NO Å activity. Ó 2004 Elsevier Ltd. All rights reserved.Abbreviations: CAPON, Carboxy-terminal PDZ ligand of nNOS; DOPAC, dihydroxyphenylacetic acid; EPR, electron paramagnetic resonance; LTP, long-term potentiation; NMDA, N-methyl-D D -aspartate; NOS, nitric oxide synthase; PDZ, PSD-95 discs large/ZO-1 homology domain; PSD-95, post-synaptic density protein 95

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Section: Measurement Of No å In Hippocampal Subregions Using Electrocmentioning

confidence: 99%

Nitric oxide in brain: diffusion, targets and concentration dynamics in hippocampal subregions

Ledo

Frade

Barbosa

et al. 2004

Molecular Aspects of Medicine

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“…Modification of the carbon electrode surface (e.g., with complex compounds or enzymes) is often needed to improve the selectivity of the sensor to other important cell analytes, such as nitrogen oxide or glucose. 15,16 Alteration of electrode properties requires analysis of the sensor response prior to performing electrochemical cell studies.…”

Section: ' Ultramicroelectrodesmentioning

confidence: 99%

“…9,10,41,42 Among the diverse range of applications, SECM has probed membrane permeability, 7,43 detected the presence of metabolites, 15,44 and evaluated enzymatic activity. 45,46 A few recent examples are briefly discussed below.…”

Section: ' Selected Demonstrationsmentioning

confidence: 99%

Biological Scanning Electrochemical Microscopy and Its Application to Live Cell Studies

et al. 2011

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“…Current methods available for detecting biologically produced NO include spectrophotometric, fluorometric, electrochemical, chemiluminescent, and electromagnetic spin resonance techniques [20]. Of these analyses however, only fluorometric and electrochemical have both the sensitivity and flexibility to measure low levels of constitutively produced NO from cultured cells in situ.…”

Section: Introductionmentioning

confidence: 99%

An Improved Assay for Measuring Low Levels of Nitric Oxide in Cultured Pulmonary Myofibroblasts

Sharma¹,

Rowland²,

Clouse³

et al. 2014

ABC

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Quantification of nitric oxide (NO) from cultured cells is a valuable tool for studying cell signaling. Detection of NO in biological fluids can be difficult however, due to its transient half-life and low physiological concentrations. In this study, we have refined an existing amperometric method to determine relative levels of accumulated nitrogen oxides (NO X ) in cell culture and have used this method to reproducibly quantify NO from cultured pulmonary myofibroblasts. Basal levels of NO produced by pulmonary myofibroblasts ranged from 0.6 nM to 20 nM and varied due to the growth conditions of the cells, i.e. higher NO concentrations were observed in differentiated cells. The constitutive eNOS isoform is primarily responsible for the observed NO accumulation in these cells since transcript levels of eNOS are 10-fold higher than the inducible iNOS form while nNOS was undetectable. Treatment of myofibroblasts with the inhibitors L-NNA and L-NAME resulted in a concentration dependent decrease in measured NOx. Overall, the improved assay presented here should be applicable to measuring NOX levels from many different cell types and under a wide variety of conditions.

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Nitric oxide measurements in biological samples

Cited by 101 publications

References 49 publications

Nitric oxide in brain: diffusion, targets and concentration dynamics in hippocampal subregions

Nitric oxide in brain: diffusion, targets and concentration dynamics in hippocampal subregions

Biological Scanning Electrochemical Microscopy and Its Application to Live Cell Studies

An Improved Assay for Measuring Low Levels of Nitric Oxide in Cultured Pulmonary Myofibroblasts

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