Nitric oxide is involved in hydrogen sulfide-induced adventitious rooting in tomato (

Abstract

Nitric oxide (NO) and hydrogen sulfide (H2S) are signalling molecules that regulate adventitious rooting in plants. However, little is known about the cross-talk between NO and H2S during adventitious rooting. Tomato (Solanum lycopersicum L.) explants were used to investigate the roles of and relationships between NO and H2S during rooting. Effects of the NO donor sodium nitroprusside (SNP) and the H2S donor sodium hydrosulfide (NaHS) on adventitious rooting were dose-dependent, and the greatest biological responses were observed under 25 μM SNP and 50 μM NaHS. The positive effect of NaHS was reversed by the NO scavenger 2-(4-carboxy-2-phenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (cPTIO), indicating that the H2S-induced response was partially NO-dependent. Peroxidase (POD), polyphenol oxidase (PPO), and superoxide dismutase (SOD) activities significantly increased by SNP and NaHS treatment, and indoleacetic acid oxidase (IAAO) activity and the O2− and H2O2 content significantly decreased by SNP and NaHS treatment. SNP and NaHS treatment also increased the content of soluble sugar and protein and indole-3-acetic acid (IAA). cPTIO significantly mitigated the increases in POD, PPO and SOD activity and soluble sugar, protein and IAA content induced by NaHS. SNP and NaHS upregulated the expression of auxin-related genes (ARF4 and ARF16), cell cycle-related genes (CYCD3, CYCA3 and CDKA1), and antioxidant-related genes (TPX2, SOD and POD); whereas cPTIO significantly inhibited the increase in the expression of these genes induced by NaHS. Overall, these results show that NO may be involved in H2S-induced adventitious rooting by regulating the activity of rooting-related enzymes, the expression of related genes, and the content of various nutrients.

RNA Sequencing Analysis and Verification of Paeonia ostii ‘Fengdan’ CuZn Superoxide Dismutase (PoSOD) Genes in Root Development

“…In this study, we found that DEGs were expressed at four different stages of root development and were enriched in various pathways (Figure 2). During the rooting process, the activities of antioxidant enzymes may enhance the plant's ability to withstand abiotic stress and contribute to the formation of ARs [35][36][37]. Therefore, researching the root development mechanisms of genes related to antioxidant enzymes will help address the question of the low propagation coefficient.…”

Free Radicals Mediated Redox Signaling in Plant Stress Tolerance

“…The increased degradation of HY5 resulted in decreased expression of the ABI5 gene, thereby enhancing seed germination under heat stress [ 68 ]. In another study, the combinatorial effect of NO and H 2 S signaling regulated the adventitious rooting in tomato plants by activating the expression of auxin-related genes (ARF4 and ARF16) and cell cycle-related genes (CYCD3, CYCA3, and CDKA1) [ 69 ]. Exogenous application of NaHS leads to the activation of transcription and activity of L-DES1 that stimulates the H 2 S biosynthesis, which in turn activates the germination of Arabidopsis seeds [ 70 ].…”

Nitric oxide promotes adventitious root formation in cucumber under cadmium stress through improving antioxidant system, regulating glycolysis pathway and polyamine homeostasis

“…Nitric oxide (NO) has been implicated as an essential signaling molecule in plants. Numerous studies have discovered that NO plays an essential role in the regulation of plant growth and development including seed germination ( Ren et al., 2020 ), root growth and development ( Pagnussat et al., 2002 ; Sun et al., 2019 ; Liu et al, 2022a ), pollen tube germination ( Prado et al., 2004 ) and fruit senescence ( Zuccarelli et al., 2021 ). The increasing evidence indicates that, NO function in plant stress response.…”