Nitric oxide involvement in the peptide VIP‐associated inhibitory junction potential in the guinea‐pig ileum.

He, Xue-Dao; Goyal, Raj K.

doi:10.1113/jphysiol.1993.sp019524

Cited by 109 publications

(98 citation statements)

References 42 publications

(93 reference statements)

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“…The serial action of both VIP and NO to effect the slow IJP in circular smooth muscle of murine stomach is similar to that reported in guinea pig ileum (18). Our findings are also consistent with mechanical studies showing that both VIP and NO are involved in EFS-induced relaxation of fundic circular smooth muscle strips (19).…”

Section: Discussionsupporting

confidence: 91%

“…All drugs were made up fresh on the day of the experiment. KRB solution and authentic NO were prepared as described elsewhere (18). The following drug concentrations were used: apamin (0.…”

Section: Methodsmentioning

confidence: 99%

“…The NANC transmission includes an excitatory junction potential mediated by SP and an inhibitory junction potential (IJP). Desensitizing SP receptors allows full expression of this IJP and the hyperpolarizing action of VIP (17,18). Electrical field stimulation (EFS) was provided using silver chloride electrodes (120 V, 2 ms duration square pulses, 10 pulses delivered over 0.5 s) as described earlier (17).…”

Section: Methodsmentioning

confidence: 99%

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Neuronal constitutive nitric oxide synthase is involved in murine enteric inhibitory neurotransmission.

Mashimo

He²,

Huang³

et al. 1996

J. Clin. Invest.

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106

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Section: Discussionsupporting

confidence: 91%

“…All drugs were made up fresh on the day of the experiment. KRB solution and authentic NO were prepared as described elsewhere (18). The following drug concentrations were used: apamin (0.…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Neuronal constitutive nitric oxide synthase is involved in murine enteric inhibitory neurotransmission.

Mashimo

He²,

Huang³

et al. 1996

J. Clin. Invest.

Self Cite

106

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“…Using SK channel and nNOS inhibition, a distinctive fast IJP and slow IJP mediated by purinergic and nitrergic nerves, respectively, have been clearly shown in both guinea pig small intestine (He and Goyal, 1993) and murine lower esophageal sphincter (Zhang et al, 2008). The purinergic fast IJPs result from opening of SK channels, whereas nitrergic slow IJPs appear to be caused by closing of Ca 2ϩ -activated Cl Ϫ channels (Crist et al, 1991;Zhang and Paterson, 2003).…”

Section: Discussionmentioning

confidence: 99%

P2Y₁ Receptors Mediate Apamin-Sensitive and -Insensitive Inhibitory Junction Potentials in Murine Colonic Circular Smooth Muscle

Zhang¹,

Lomax²,

Paterson³

2010

J Pharmacol Exp Ther

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Purinergic inhibitory neuromuscular transmission plays an important role in the control of intestinal motility. In most tissues this neurotransmission is apamin-sensitive, but recent studies in human colonic circular smooth muscle (CSM) suggest the presence of apamin-insensitive purinergic inhibitory junction potentials (IJPs). The current studies used conventional intracellular recordings on colonic CSM strips to characterize the purinergic IJPs in murine colonic CSM. P2Y 1 receptor expression was examined by using reverse transcriptase-polymerase chain reaction (RT-PCR) and immunohistochemistry. The IJP induced by nerve stimulation (NS) of one and four pulses in neuronal nitric-oxide synthase knockout mice consists of an apamin-sensitive and a dominant apamin-resistant component. These are identical to the IJPs in wild-type and CD1 mice in the presence of N -nitro-L-arginine methyl ester (200 M) and were significantly inhibited by ␣,␤-methylene ATP (50 M), an analog of ATP. IJPs were not affected by the P2X receptor antagonist 2Ј,3Ј-o-(2,4,6-trinitrophenyl)-ATP (10 M). Furthermore, apamin-resistant IJPs induced by singlepulse NS were abolished by pyridoxal-phosphate-6-azophenyl-2Ј,4Ј-disulfonate (100 M), a P2 receptor antagonist; 2Ј-deoxy-N6-methyl adenosine 3,5-diphosphate (MRS-2179; 10 M), a selective P2Y 1 receptor antagonist; and tetrodotoxin (1 M). Aboral NS induced apaminsensitive purinergic IJPs, whereas oral and circumferential NS produced apamin-sensitive and -resistant IJPs, with the latter predominating. RT-PCR and immunohistochemistry confirmed the presence of P2Y 1 receptors on smooth muscle and in the myenteric plexus. These data suggest that, depending on stimulus location, activation of P2Y 1 receptors produces both apamin-sensitive and apamin-resistant IJPs in murine colonic CSM.

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“…Inhibitory motor neuron activation relaxes intestinal smooth muscle [86,92,94], and this relaxation is markedly reduced IJP blockade in some preparations (for examples see [95][96][97][98]). The inhibitory motor neurons contain nitric oxide synthase (NOS) [99], and blockade of this enzyme prevents the prolonged hyperpolarisations and depresses smooth-muscle relaxation, but not the IJPs, evoked by their activity [87,90,93,98,100]. The IJPs are blocked by the bee venom toxin apamin, an antagonist of the SK form of calcium-dependent potassium channels [95,96,101].…”

Section: Inhibitory Neuromuscular Transmissionmentioning

confidence: 99%

Purinergic mechanisms in the control of gastrointestinal motility

Bornstein

2007

Purinergic Signalling

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For many years, ATP and adenosine have been implicated in movement regulation of the gastrointestinal tract. They act through three major receptor subtypes: adenosine or P1 receptors, P2X receptors and P2Y receptors. Each of these major receptor types can be subdivided into several different classes and is widely distributed amongst various neurons, muscle types, glia and interstitial cells that regulate intestinal functions. Several key roles for the different receptors and their endogenous ligands have been identified in physiological and pharmacological studies. For example, adenosine acting at A 1 receptors appears to inhibit intestinal motility in various pathological conditions. Similarly, ATP acting at P2Y receptors is an important component of inhibitory neuromuscular transmission, acting as a cotransmitter with nitric oxide. ATP acting at P2X and P2Y 1 receptors is important for synaptic transmission in simple descending excitatory and inhibitory reflex pathways. Some P2Y receptor subtypes prefer uridine nucleotides over purine nucleotides. Thus, roles for UTP and UDP as enteric transmitters in place of ATP cannot be excluded. ATP also appears to be important for sensory transduction, especially in chemosensitive pathways that initiate local inhibitory reflexes. Despite this evidence, data are lacking about the roles of either adenosine or ATP in more complex motility patterns such as segmentation or the interdigestive migrating motor complex. Clarification of roles for purinergic transmission in these common, but understudied, motility patterns will depend on the use of subtype-specific antagonists that in some cases have not yet been developed.

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Nitric oxide involvement in the peptide VIP‐associated inhibitory junction potential in the guinea‐pig ileum.

Cited by 109 publications

References 42 publications

Neuronal constitutive nitric oxide synthase is involved in murine enteric inhibitory neurotransmission.

Neuronal constitutive nitric oxide synthase is involved in murine enteric inhibitory neurotransmission.

P2Y₁ Receptors Mediate Apamin-Sensitive and -Insensitive Inhibitory Junction Potentials in Murine Colonic Circular Smooth Muscle

Purinergic mechanisms in the control of gastrointestinal motility

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Nitric oxide involvement in the peptide VIP‐associated inhibitory junction potential in the guinea‐pig ileum.

Cited by 109 publications

References 42 publications

Neuronal constitutive nitric oxide synthase is involved in murine enteric inhibitory neurotransmission.

Neuronal constitutive nitric oxide synthase is involved in murine enteric inhibitory neurotransmission.

P2Y1 Receptors Mediate Apamin-Sensitive and -Insensitive Inhibitory Junction Potentials in Murine Colonic Circular Smooth Muscle

Purinergic mechanisms in the control of gastrointestinal motility

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P2Y₁ Receptors Mediate Apamin-Sensitive and -Insensitive Inhibitory Junction Potentials in Murine Colonic Circular Smooth Muscle