Nitric oxide interacts with the cAMP pathway to modulate capacitation of human spermatozoa

Herrero, Marı́a Belén; Chatterjee, Suvro; Lefièvre, Linda; Lamirande, Eve de; Gagnon, Claude

doi:10.1016/s0891-5849(00)00339-7

Cited by 101 publications

(85 citation statements)

References 64 publications

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“…On the other hand, high NO concentrations (25-125 mM pure NO, 0.25-2.5 mM sodium nitroprusside, 12-600 mM S-nitroso-N-acetylpenicillamine, and 100-125 mM 3-morpholinosydnonimine) seem to exert opposite effects on the motility of human spermatozoa in vitro (Rosselli et al 1995, Weinberg et al 1995, Nobunaga et al 1996. Studies on sperm capacitation showed that NO (1-100 mM spermine NONOate (SPNO) or diethylamine-NONOate) increases cAMP levels, thus triggering protein kinase A activation and tyrosine phosphorylation (Herrero et al 2000) and is also involved in activation of protein ERKs (Thundathil et al 2003, O'Flaherty et al 2006. On the other hand, like in many other cell types, NO activates the soluble guanylate cyclase (sGC) in human spermatozoa (Revelli et al 2002).…”

Section: Introductionmentioning

confidence: 99%

Nitric oxide stimulates human sperm motility via activation of the cyclic GMP/protein kinase G signaling pathway

Miraglia¹,

Angelis²,

Gazzano³

et al. 2011

Reproduction

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Nitric oxide (NO), a modulator of several physiological processes, is involved in different human sperm functions. We have investigated whether NO may stimulate the motility of human spermatozoa via activation of the soluble guanylate cyclase (sGC)/cGMP pathway. Sperm samples obtained by masturbation from 70 normozoospermic patients were processed by the swim-up technique. The kinetic parameters of the motile sperm-rich fractions were assessed by computer-assisted sperm analysis. After a 30-90 min incubation, the NO donor S-nitrosoglutathione (GSNO) exerted a significant enhancing effect on progressive motility (77, 78, and 78% vs 66, 65, and 62% of the control at the corresponding time), straight linear velocity (44, 49, and 48 mm/s vs 34, 35, and 35.5 mm/s), curvilinear velocity (81, 83, and 84 mm/s vs 68 mm/s), and average path velocity (52, 57, and 54 mm/s vs 40, 42, and 42 mm/s) at 5 mM but not at lower concentrations, and in parallel increased the synthesis of cGMP. A similar effect was obtained with the NO donor spermine NONOate after 30 and 60 min. The GSNO-induced effects on sperm motility were abolished by 1H-[1,2,4]oxadiazolo-[4,3-a]quinoxalin-1-one (a specific sGC inhibitor) and mimicked by 8-bromo-cGMP (8-Br-cGMP; a cell-permeating cGMP analog); the treatment with Rp-8-Br-cGMPS (an inhibitor of cGMP-dependent protein kinases) prevented both the GSNO-and the 8-Br-cGMP-induced responses. On the contrary, we did not observe any effect of the cGMP/PRKG1 (PKG) pathway modulators on the onset of hyperactivated sperm motility. Our results suggest that NO stimulates human sperm motility via the activation of sGC, the subsequent synthesis of cGMP, and the activation of cGMP-dependent protein kinases.

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Section: Introductionmentioning

confidence: 99%

Nitric oxide stimulates human sperm motility via activation of the cyclic GMP/protein kinase G signaling pathway

Miraglia¹,

Angelis²,

Gazzano³

et al. 2011

Reproduction

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“…Although SOD restricts ONOO -formation through the dismutation of O 2 -, it catalyses nitration (Schopfer et al, 2003). Nitration affects protein status and contributes to several signal transduction pathways in mammalian sperm (Aitken et al, 1995;de Lamirande and Gagnon, 1995;de Lamirande et al, 1997;Aitken et al, 1998;de Lamirande and Gagnon, 1998;de Lamirande et al, 1998a;Herrero et al, 2000;Herrero and Gagnon, 2001;O'Flaherty et al, 2006). In mammalian sperm, nitration affects tyrosine phosphorylation, which is involved in capacitation (Aitken et al, 1995;de Lamirande et al, 1997;O'Flaherty et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

“…In mammalian sperm, peroxynitrite (ONOO -), which is formed by the reaction of nitric oxide (NO) with the superoxide anion (O 2 -), attaches to tyrosine residues in sperm proteins ('nitration'), thereby affecting capacitation (Aitken et al, 1995;de Lamirande and Gagnon, 1995;de Lamirande et al, 1998a;de Lamirande et al, 1998b;Belen Herrero et al, 2000;de Lamirande and Gagnon, 2002). The amount of O 2 -present determines the amount of ONOOproduced (Wink and Mitchell, 1998).…”

Section: Reactive Oxygen Species Production In Spermmentioning

confidence: 99%

Involvement of redox- and phosphorylation-dependent pathways in osmotic adaptation in sperm cells of euryhaline tilapia

Morita

Nakajima

Takemura

et al. 2011

Journal of Experimental Biology

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SUMMARYSperm cells involved in fertilisation must tolerate hypo-osmotic and hyper-osmotic environments. Euryhaline tilapia (Oreochromis mossambicus) can acclimatise to and reproduce in freshwater and seawater because its sperm are able to adapt to these differing osmotic environments. In this study, we found that the dephosphorylation of sperm proteins in O. mossambicus correlated with the activation of flagellar motility when sperm were exposed to hypotonic or hypertonic conditions, and that differences in phosphorylation may reflect adaptations to a given osmotic environment. Of the sperm proteins that were dephosphorylated, the phosphorylation pattern of an 18kDa protein, identified as the superoxide anion scavenger Cu/Zn superoxide dismutase (Cu/Zn SOD), was different in freshwater-and seawater-acclimatised tilapia sperm. Cu/Zn SOD was distributed from the sperm head to the flagellum. Additionally, differences were observed between freshwater and seawater tilapia in the nitration of tyrosine residues (which might be mediated by SOD) in sperm flagellar proteins in response to osmotic shock. These results demonstrate that reactive-oxygen-species-dependent mechanisms contribute to both osmotic tolerance and the activation of flagellar motility.

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“…In studies using a variety of mammals, ROS were harmful to the sperm of some species [16], while in other species low levels of ROS were beneficial [14]. For example, nitric oxide (NO) in sperm promotes motility [16,21], maturation [9,13], capacitation [7,8,19], hyperactivation [4,23], and the acrosome reaction [6,20]. In human spermatozoa, NO is required for successful binding to the zona pellucida [5,17], and in rabbits the NO=NOS system is involved in follicle rupture during the ovulatory process [22].…”

Section: Introductionmentioning

confidence: 99%

Detection and Bioimaging of Nitric Oxide in Bovine Oocytes and Sperm Cells

Reyes

Vázquez²,

Delgado³

2004

Archives of Andrology

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Mammalian gametes contain constitutive nitric oxide synthases (NOS) to synthesize nitric oxide (NO). The detection and bioimaging of NO in bovine gametes is important to determine the regulatory roles of NO during the different events of fertilization. Diaminofluoresceins, new fluorescence indicators for NO, were applied to detect the release of NO from bovine gametes. These compounds yield green fluorescent triazolofluoresceins, which provide the advantages of high specificity, sensitivity, and simplicity for the detection of NO. In this study, we mapped the expression of NOS in the bovine sperm and ova. NOS activity in sperm first appeared in the acrosome, then 60 min later in the head, middle piece, cytoplasmic droplet, and tail. Cow ova had high NO activity in the cytoplasm and in the surrounding corona cells, but not in the zona pellucida. These results show that for bovine gametes, the synthesis NO by the NOS system presents clear patterns of time and spatial distribution that may be important for the different events of fertilization.

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Nitric oxide interacts with the cAMP pathway to modulate capacitation of human spermatozoa

Cited by 101 publications

References 64 publications

Nitric oxide stimulates human sperm motility via activation of the cyclic GMP/protein kinase G signaling pathway

Nitric oxide stimulates human sperm motility via activation of the cyclic GMP/protein kinase G signaling pathway

Involvement of redox- and phosphorylation-dependent pathways in osmotic adaptation in sperm cells of euryhaline tilapia

Detection and Bioimaging of Nitric Oxide in Bovine Oocytes and Sperm Cells

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