Nitric oxide-independent vasodilator rescues heme-oxidized soluble guanylate cyclase from proteosomal degradation

Meurer, Sabine; Pioch, Sylke; Pabst, Tatjana; Opitz, Nils; Schmidt, Peter M.; Beckhaus, Tobias; Wagner, Kristina; Matt, Simone; Gegenbauer, Kristina; Geschka, Sandra; Karas, Michael; Stasch, Johannes‐Peter; Schmidt, Harald; Müller‐Esterl, Werner

doi:10.1186/1471-2210-9-s1-p49

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“…An increased level of ROS is associated with oxidation of the β 1 -subunit prosthetic heme group that converts sGC to an NO-insensitive state (Stasch et al ., 2006). Under inflammatory conditions, sGC is not sensible to activation by NO (Meurer et al ., 2009). In the presence of excess levels of ROS, NO-dependent S-nitrosylation of a β 1 -subunit-thiol resulted in decreased responsiveness of sGC to NO (Sayed et al ., 2007).…”

Section: Discussionmentioning

confidence: 99%

Irreversible Inflammation is Associated with Decreased Levels of the α₁-, β₁-, and α₂-Subunits of sGC in Human Odontoblasts

et al. 2011

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The nitric oxide (NO) receptor enzyme soluble guanylate cyclase (sGC) contains one prosthetic heme group as an αβ heterodimer, and two heterodimer isoforms (α(1)β(1), α(2)β(1)) were characterized to have enzyme activity. To test the irreversible inflammation-dependent regulation of sGC in odontoblasts, we incubated decalcified frozen sections of healthy and inflamed human third molars with antibodies against β-actin, nitrotyrosine, inducible nitric oxide synthase (iNOS), α(1)-, β(1)-, and α(2)-subunits of sGC and analyzed them at protein levels by quantitative immunohistochemistry. The irreversible inflammation induced an increase in the signal intensities for nitrotyrosine and iNOS and a decrease for the α(1)-, β(1)-, and α(2)-subunits of sGC in odontoblasts. Inflammatory mediators, reactive oxygen, and nitrogen species may impair the expression of the α(1)-, β(1)-, and α(2)-subunits in odontoblasts. The decrease of sGC at the protein level in inflamed odontoblasts is compatible with a critical role for sGC to mediate biological effects of NO in health.

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Section: Discussionmentioning

confidence: 99%

Irreversible Inflammation is Associated with Decreased Levels of the α₁-, β₁-, and α₂-Subunits of sGC in Human Odontoblasts

et al. 2011

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show abstract

Nitric oxide-independent vasodilator rescues heme-oxidized soluble guanylate cyclase from proteosomal degradation

Cited by 1 publication

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Irreversible Inflammation is Associated with Decreased Levels of the α₁-, β₁-, and α₂-Subunits of sGC in Human Odontoblasts

Irreversible Inflammation is Associated with Decreased Levels of the α₁-, β₁-, and α₂-Subunits of sGC in Human Odontoblasts

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Nitric oxide-independent vasodilator rescues heme-oxidized soluble guanylate cyclase from proteosomal degradation

Cited by 1 publication

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Irreversible Inflammation is Associated with Decreased Levels of the α1-, β1-, and α2-Subunits of sGC in Human Odontoblasts

Irreversible Inflammation is Associated with Decreased Levels of the α1-, β1-, and α2-Subunits of sGC in Human Odontoblasts

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Irreversible Inflammation is Associated with Decreased Levels of the α₁-, β₁-, and α₂-Subunits of sGC in Human Odontoblasts

Irreversible Inflammation is Associated with Decreased Levels of the α₁-, β₁-, and α₂-Subunits of sGC in Human Odontoblasts