Nitric oxide in biological fluids: analysis of nitrite and nitrate by high-performance ion chromatography

Everett, Steven A.; Dennis, Madeleine F.; Tozer, Gillian M.; Prise, Vivien E.; Wardman, Peter; Stratford, Michael R.L.

doi:10.1016/0021-9673(95)00078-2

Cited by 82 publications

(40 citation statements)

References 26 publications

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“…Massspectrometry-based methods are presently the most accurate methods for quantitative determination of nitrate and nitrite in biological fluids. Also, other analytical methods, such as gas and liquid chromatography and electrophoresis, are also applied effectively [14][15][16][17][18].…”

Section: Abbreviationsmentioning

confidence: 99%

Oxidative Stress, Nitric Oxide, and Diabetes

Pitocco¹,

Zaccardi²,

Stasio³

et al. 2010

Rev Diabet Stud

276

194

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■ AbstractIn the recent decades, oxidative stress has become focus of interest in most biomedical disciplines and many types of clinical research. Increasing evidence from research on several diseases show that oxidative stress is associated with the pathogenesis of diabetes, obesity, cancer, ageing, inflammation, neurodegenerative disorders, hypertension, apoptosis, cardiovascular diseases, and heart failure. Based on this research, the emerging concept is that oxidative stress is the "final common pathway", through which risk factors of several diseases exert their deleterious effects. Oxidative stress causes a complex dysregulation of cell metabolism and cell-cell homeostasis. In this review, we discuss the role of oxidative stress in the pathogenesis of insulin resistance and beta-cell dysfunction. These are the two most relevant mechanisms in the pathophysiology of type 2 diabetes, and in the pathogenesis of diabetic vascular complications, the leading cause of death in diabetic patients.

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Section: Abbreviationsmentioning

confidence: 99%

Oxidative Stress, Nitric Oxide, and Diabetes

Pitocco¹,

Zaccardi²,

Stasio³

et al. 2010

Rev Diabet Stud

276

194

View full text Add to dashboard Cite

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“…Samples were prepared as described by Everett et al 14 Briefly, 100 ml of buffer solution were taken from the vascular chamber at cumulative Ach concentration of 10 À5 mM and subsequently 100 ml of acetonitrile were added, and then the sample mixed and centrifuged. A 50-ml volume of sample was injected into the high-performance liquid chromatography system.…”

Section: Aortic Rings Incubationmentioning

confidence: 99%

Adiponectin directly improves endothelial dysfunction in obese rats through the AMPK–eNOS Pathway

et al. 2009

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Objective: Hypoadiponectinemia has been proved to be closely related to endothelial dysfunction in peripheral arteries and is thought to be an independent risk factor for cardiovascular disease. The objective of this study was to investigate whether adiponectin might independently improve endothelial dysfunction in aorta isolated from high-fat-diet-induced obese. SpragueDawley rat and to study the mechanism involved. Research Design and Subjects: Male Sprague-Dawley rats were fed with a regular or a high-fat diet for 6 weeks. The aorta was isolated, and vascular segments were incubated with vehicle or the globular adiponectin (globular domain (gAD); 2 mg ml À1 ) for 2 h. The effect of gAD on endothelial function and nitric oxide (NO) production was determined. Human aortic endothelial cells in primary culture were treated with vehicle or gAD (4 mg ml À1 ). The effect of gAD on the level of phosphorylation of endothelial nitric oxide synthase (eNOS) at Ser1177, AMPK at Thr176 and Akt at Ser473 in endothelial cells were determined. Results: Severe endothelial dysfunction was observed in high-fat diet fed rat aortic segments. After gAd incubation, the endothelium-dependent relaxation was partly improved and total production of nitric oxide as result of enhanced eNOS activity was also increased. In the cultured endothelial cell line HUVEC, globular adiponectin increased the activity of eNOS through activating AMPK by stimulating its phosphorylation at Thr176 but not Akt. Conclusion:The demonstration in the current study that adiponectin reverses endothelial dysfunction through increasing NO production by eNOS phosphorylation, and decreasing NO inactivation by blocking superoxide production provides a new direction in the prevention of vascular injury in the obesity population.

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“…In the previous study, high performance ion chromatography was used to detect nitrite and nitrate in the foodstuffs, biological fluids and seawater (Santillana et al, 1993;Tozer et al, 1995;Horioka et al, 2014). In this study, the anions NO 2 -and NO 3 -in celeries and carrots were determined with the technique of IC and a conductivity detector.…”

Section: Discussionmentioning

confidence: 99%

“…High performance ion chromatography was used to detect nitrite and nitrate in the foodstuffs, biological fluids and seawater (Santillana et al, 1993;Tozer et al, 1995;Horioka et al, 2014). In addition, the kinetic studies were performed with ion-pair chromatography to detect nitrite and nitrate in rats (Stein et al, 1988).…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Detection of Nitrite and Nitrate in Celeries and Carrots

Liu¹,

Niu²,

Kong³

et al. 2016

American Journal of Biochemistry and Biotechnology

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Abstract:In this study, the anions nitrite ion (NO 2 -) and nitrate ion (NO 3 -) in celeries and carrots were detected with the technique of ion chromatography. A Dionex ICS-2000 ion chromatograph was used to detect the content of NO 2 -and NO 3 -in celeries and carrots. NO 2 -and NO 3 -were separated on a DIONEX Ionpac AS19 A-4 mm ion-exchange column and an Iopac AG AS19 A-4 mm guard column. The eluant was suppressed with an anion electrical self-regenerating suppressor (ASRS 300) and the content of NO 2 -and NO 3 -was detected with a conductivity detector. The detection limits were obtained for anions NO 2 -and NO 3 -. The present results showed that the ion chromatography was a suitable technique for the simultaneous determination of NO 2 -and NO 3 -in vegetables.

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Nitric oxide in biological fluids: analysis of nitrite and nitrate by high-performance ion chromatography

Cited by 82 publications

References 26 publications

Oxidative Stress, Nitric Oxide, and Diabetes

Oxidative Stress, Nitric Oxide, and Diabetes

Adiponectin directly improves endothelial dysfunction in obese rats through the AMPK–eNOS Pathway

Simultaneous Detection of Nitrite and Nitrate in Celeries and Carrots

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