Nitric oxide effectively orchestrates postharvest flower senescence: a case study of

Haq, Aehsan Ul; Lone, Mohammad Lateef; Farooq, Sumira; Parveen, Shazia; Altaf, Foziya; Tahir, Inayatullah; Hefft, Daniel Ingo; Ahmad, Ajaz; Ahmad, Parvaiz

doi:10.1071/fp21241

Cited by 12 publications

(2 citation statements)

References 67 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, the effects of GSNOR and NO on soluble sugar and soluble protein accumulation are different. A previous study found that NO alleviated postharvest senescence in Consolida ajacis cut flowers by improving the levels of sugars, phenols and soluble proteins [ 41 ]. Nevertheless, another report found that the bioproduction of sugars, organic acids, and amino acids was mildly influenced by exogenous NO and indirectly stimulated GSNOR during tomato storage [ 30 ].…”

Section: Discussionmentioning

confidence: 99%

The Crucial Role of SlGSNOR in Regulating Postharvest Tomato Fruit Ripening

Liu,

Huang,

Yao

et al. 2024

IJMS

View full text Add to dashboard Cite

S-nitrosoglutathione reductase (GSNOR) is a well-known regulator in controlling protein S-nitrosylation modification and nitric oxide (NO) homeostasis. Here, a GSNOR inhibitor N6022 and SlGSNOR silencing were applied to investigate the roles of SlGSNOR in tomato fruit postharvest ripening. We found that the application of N6022 and S-nitrosoglutathione (GSNO, a NO donor), and SlGSNOR silencing delayed the transition of fruit skin color by improving total chlorophyll level by 88.57%, 44.78%, and 91.03%, respectively. Meanwhile, total carotenoid and lycopene contents were reduced by these treatments. Concurrently, the activity of chlorophyll biosynthesis enzymes and the expression of related genes were upregulated, and the transcript abundances of total carotenoid bioproduction genes were downregulated, by N6022 and GSNO treatments and SlGSNOR silencing. In addition, fruit softening was postponed by N6022, GSNO, and SlGSNOR silencing, through delaying the decrease of firmness and declining cell wall composition; structure-related enzyme activity; and gene expression levels. Furthermore, N6022, GSNO, and SlGSNOR silencing enhanced the accumulation of titratable acid; ascorbic acid; total phenol; and total flavonoid, but repressed the content of soluble sugar and soluble protein accompanied with the expression pattern changes of nutrition-related genes. In addition, the endogenous NO contents were elevated by 197.55%; 404.59%; and 713.46%, and the endogenous SNOs contents were enhanced by 74.65%; 93.49%; and 94.85%; by N6022 and GSNO treatments and SlGSNOR silencing, respectively. Altogether, these results indicate that SlGSNOR positively promotes tomato postharvest fruit ripening, which may be largely on account of its negative roles in the endogenous NO level.

show abstract

Section: Discussionmentioning

confidence: 99%

The Crucial Role of SlGSNOR in Regulating Postharvest Tomato Fruit Ripening

Liu,

Huang,

Yao

et al. 2024

IJMS

View full text Add to dashboard Cite

show abstract

“…In an early proteomic study of potential targets of S -nitrosation in potato tuber samples treated with the NO donor S -nitrosoglutathione (GSNO), it was possible to identify 80 protein candidates and, among them, was found a LOX protein [ 52 ]. The NO treatment of the cut Consolida ajacis flower during postharvest storage triggered a decrease in LOX activity, which alleviated its deterioration since the NO promoted the membrane stability [ 53 ]. In Arabidopsis, NO induces the expression of the LOX3 gene, which leads to JA production [ 54 ].…”

Section: Discussionmentioning

confidence: 99%

Lipoxygenase (LOX) in Sweet and Hot Pepper (Capsicum annuum L.) Fruits during Ripening and under an Enriched Nitric Oxide (NO) Gas Atmosphere

González‐Gordo

Cañas

Muñoz‐Vargas

et al. 2022

IJMS

View full text Add to dashboard Cite

Lipoxygenases (LOXs) catalyze the insertion of molecular oxygen into polyunsaturated fatty acids (PUFA) such as linoleic and linolenic acids, being the first step in the biosynthesis of a large group of biologically active fatty acid (FA)-derived metabolites collectively named oxylipins. LOXs are involved in multiple functions such as the biosynthesis of jasmonic acid (JA) and volatile molecules related to the aroma and flavor production of plant tissues, among others. Using sweet pepper (Capsicum annuum L.) plants as a model, LOX activity was assayed by non-denaturing polyacrylamide gel electrophoresis (PAGE) and specific in-gel activity staining. Thus, we identified a total of seven LOX isozymes (I to VII) distributed among the main plant organs (roots, stems, leaves, and fruits). Furthermore, we studied the FA profile and the LOX isozyme pattern in pepper fruits including a sweet variety (Melchor) and three autochthonous Spanish varieties that have different pungency levels (Piquillo, Padrón, and Alegría riojana). It was observed that the number of LOX isozymes increased as the capsaicin content increased in the fruits. On the other hand, a total of eight CaLOX genes were identified in sweet pepper fruits, and their expression was differentially regulated during ripening and by the treatment with nitric oxide (NO) gas. Finally, a deeper analysis of the LOX IV isoenzyme activity in the presence of nitrosocysteine (CysNO, a NO donor) suggests a regulatory mechanism via S-nitrosation. In summary, our data indicate that the different LOX isozymes are differentially regulated by the capsaicin content, fruit ripening, and NO.

show abstract