Nitric oxide downregulates tumour necrosis factor mRNA in RAW 264.7 cells

Sinha, Bhanu; Eigler, Andreas; Baumann, Kaspar; Greten, Tim F.; Moeller, J; Endres, Stefan

doi:10.1016/s0923-2494(98)80297-6

Cited by 34 publications

(22 citation statements)

References 43 publications

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“…High cGMP concentrations can cross-activate protein kinase A and lead to ERK as well as Raf-1 inhibition (36,37). Also, destabilization of TNF-␣ mRNA by cGMP in LPS-activated macrophages was discussed previously (38). The inhibition of TNF gene expression by atrial natriuretic peptide has been found to be a cGMP-mediated effect, involving inhibition of AP-1 and NF-B activation (12,39).…”

Section: Discussionmentioning

confidence: 88%

Zinc-Mediated Inhibition of Cyclic Nucleotide Phosphodiesterase Activity and Expression Suppresses TNF-α and IL-1β Production in Monocytes by Elevation of Guanosine 3′,5′-Cyclic Monophosphate

Bülow

Rink

Haase

2005

The Journal of Immunology

141

110

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The trace element zinc affects several aspects of immune function, such as the release of proinflammatory cytokines from monocytes. We investigated the role of cyclic nucleotide signaling in zinc inhibition of LPS-induced TNF-α and IL-1β release from primary human monocytes and the monocytic cell line Mono Mac1. Zinc reversibly inhibited enzyme activity of phosphodiesterase-1 (PDE-1), PDE-3, and PDE-4 in cellular lysate. It additionally reduced mRNA expression of PDE-1C, PDE-4A, and PDE-4B in intact cells. Although these PDE can also hydrolyze cAMP, only the cellular level of cGMP was increased after incubation with zinc, whereas cAMP was found to be even slightly reduced due to inhibition of its synthesis. To investigate whether an increase in cGMP alone is sufficient to inhibit cytokine release, the cGMP analogues 8-bromo-cGMP and dibutyryl cGMP as well as the NO donor S-nitrosocysteine were used. All three treatments inhibited TNF-α and IL-1β release after stimulation with LPS. Inhibition of soluble guanylate cyclase-mediated cGMP synthesis with LY83583 reversed the inhibitory effect of zinc on LPS-induced cytokine release. In conclusion, inhibition of PDE by zinc abrogates the LPS-induced release of TNF-α and IL-1β by increasing intracellular cGMP levels.

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Section: Discussionmentioning

confidence: 88%

Zinc-Mediated Inhibition of Cyclic Nucleotide Phosphodiesterase Activity and Expression Suppresses TNF-α and IL-1β Production in Monocytes by Elevation of Guanosine 3′,5′-Cyclic Monophosphate

Bülow

Rink

Haase

2005

The Journal of Immunology

141

110

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“…and their LPS stimulate TNF and NO production via expression of iNOS in macrophages. 41,42) This study showed that HCWE activates bacterial uptake and simultaneously induces NO production and iNOS expression in RAW 264.7 cells. It appears that the phagocytic activation of macrophages is directly affected by HCWE and results in increased internalization of Salmonella by macrophages.…”

Section: )mentioning

confidence: 86%

Biological and Antibacterial Activities of the Natural Herb <i>Houttuynia cordata</i> Water Extract against the Intracellular Bacterial Pathogen <i>Salmonella</i> within the RAW 264.7 Macrophage

Kim

Lim

et al. 2008

Biological & Pharmaceutical Bulletin

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Houttuynia cordata has a long history of use in indigenous systems of medicine as an antiseptic, febrifuge, diuretic, and deobstruant. It is frequently used in many traditional medicines for its antimicrobial, antiviral, and antiinflammatory properties. [1][2][3][4] H. cordata contains of methyl nonyl ketone, caryophyllene, bornyl acetate, a-pinene, b-pinene, limonene, and other components. These components have antibacterial properties against the Gram-positive bacteria Staphylococcus aureus and Sarcina ureae. 5) In experimental studies, H. cordata exhibited antibacterial activity, antileukemic activity, antiinflammatory activity, anaphylactic reaction and mast cell activation, and modulated nitric oxide (NO) production. [6][7][8][9][10] However, to date, studies to understand the other antibacterial effects, including the intracellularly replicating pathogens of these plants, are lacking.The mammalian immune response is divided broadly into the innate and adaptive responses. Innate immune responses of mammals involve the first line of defense against invading microorganisms, including the release of antimicrobial peptides at epithelial surfaces, phagocytosis, and intracellular killing of microorganisms by phagocytes and the activation of the complement cascade. In contrast, the adaptive immune response is antigen specific and modified by previous antigen exposure. 11) In the innate immune response, the reactive oxygen intermediates (ROIs) such as superoxide and reactive nitrogen intermediates (RNIs) such as NO have a key role in intracellular killing of microorganisms within phagocytes. 12)Several studies reported that RNIs are important for controlling Salmonella multiplication within macrophages. 13,14)Salmonellosis is a major bacterial zoonosis that causes a variety of disease syndromes, from self-limiting enteritis to fatal infection in animals and food-borne infection and typhoid fever in humans.15) The etiologic agents of salmonellosis are Salmonella sp. characterized by motile, Gram-negative, rod-shaped bacteria and facultative intracellular pathogens that can multiply within professional and nonprofessional phagocytes. Recently, the emergence of multidrugresistant strains of Salmonella sp. has caused more serious problems in public health.16) Multidrug resistance is a worldwide problem that does not recognize international borders and can indiscriminately affect the treatment and prevention of various diseases.To kill intracellular bacteria including Salmonella sp., Brucella sp., Listeria sp., Shigella sp., etc., much effort is needed to develop antibiotics, determine treatment duration, and resolve other issues.In this study, we examined the antibacterial effects, and macrophage activation of H. cordata water extract (HCWE) such as morphologic changes, NO production, phagocytic activity, and effects against the Salmonella enterica serovar Typhimurium in vitro and in vivo. Salmonellosis is a major bacterial zoonosis that causes a variety of disease syndromes, from self-limiting enteritis to fatal infec...

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“…They also demonstrated that the IFN-␥-induced enhancement of anti-Brucella activities by murine peritoneal macrophages was inhibited by the addition of anti-TNF-␣ antibodies to the cultures, indicating that TNF-␣ is necessary for full expression of macrophage anti-Brucella activities. This is interesting considering that in RAW264.7 cells, endogenous NO has a negative feedback effect on TNF-␣ synthesis in vitro, mainly by reducing the half-life of TNF-␣ mRNA (19,55). These findings underscore the complexity of the relationship between the host and intracellular parasites that cannot be reduced to the expression of a single gene or the production of a single antimicrobial factor.…”

Section: Vol 69 2001mentioning

confidence: 97%

Stable Transfection of the BovineNRAMP1Gene into Murine RAW264.7 Cells: Effect onBrucella abortusSurvival

Barthel¹,

Feng²,

Piedrahita³

et al. 2001

Infect Immun

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Genetically based natural resistance to brucellosis in cattle provides for novel strategies to control zoonotic diseases. Bovine NRAMP1, the homologue of a murine gene (Bcg), has been identified as a major candidate for controlling the in vivo resistant phenotype. We developed an in vitro model for expression of resistance-and susceptibility-associated alleles of bovine NRAMP1 as stable transgenes under the regulatory control of the bovine NRAMP1 promoter in the murine RAW264.

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Nitric oxide downregulates tumour necrosis factor mRNA in RAW 264.7 cells

Cited by 34 publications

References 43 publications

Zinc-Mediated Inhibition of Cyclic Nucleotide Phosphodiesterase Activity and Expression Suppresses TNF-α and IL-1β Production in Monocytes by Elevation of Guanosine 3′,5′-Cyclic Monophosphate

Zinc-Mediated Inhibition of Cyclic Nucleotide Phosphodiesterase Activity and Expression Suppresses TNF-α and IL-1β Production in Monocytes by Elevation of Guanosine 3′,5′-Cyclic Monophosphate

Biological and Antibacterial Activities of the Natural Herb <i>Houttuynia cordata</i> Water Extract against the Intracellular Bacterial Pathogen <i>Salmonella</i> within the RAW 264.7 Macrophage

Stable Transfection of the BovineNRAMP1Gene into Murine RAW264.7 Cells: Effect onBrucella abortusSurvival

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