Nitric oxide detection methods in vitro and in vivo

Goshi, Ekta; Zhou, Gaoxin; He, Qianjun

doi:10.4103/2045-9912.273957

Cited by 77 publications

(67 citation statements)

References 113 publications

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“…The NO concentration was determined using Griess assay. According to previous papers, nitrite is the stable end product of nitric oxide radical, and widely used as the indicator of NO 64,65 . Briefly, 50 µL of LPS-treated BV-2 cell medium or mouse brain homogenate was incubated with 50 µL of Griess reagent (sulfanilamine in 1% H 3 PO 4 /0.1% N-(1-naphthyl)-ethylenediamine dihydrochloride/1% H 3 PO 4 /distilled water, 1:1:1:1) at room temperature for 10 min.…”

Section: Methodsmentioning

confidence: 99%

Facilitation of molecular motion to develop turn-on photoacoustic bioprobe for detecting nitric oxide in encephalitis

Feng

Zhang

et al. 2021

Nat Commun

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Nitric oxide (NO) is an important signaling molecule overexpressed in many diseases, thus the development of NO-activatable probes is of vital significance for monitoring related diseases. However, sensitive photoacoustic (PA) probes for detecting NO-associated complicated diseases (e.g., encephalitis), have yet to be developed. Herein, we report a NO-activated PA probe for in vivo detection of encephalitis by tuning the molecular geometry and energy transformation processes. A strong donor-acceptor structure with increased conjugation can be obtained after NO treatment, along with the active intramolecular motion, significantly boosting “turn-on” near-infrared PA property. The molecular probe exhibits high specificity and sensitivity towards NO over interfering reactive species. The probe is capable of detecting and differentiating encephalitis in different severities with high spatiotemporal resolution. This work will inspire more insights into the development of high-performing activatable PA probes for advanced diagnosis by making full use of intramolecular motion and energy transformation processes.

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Section: Methodsmentioning

confidence: 99%

Facilitation of molecular motion to develop turn-on photoacoustic bioprobe for detecting nitric oxide in encephalitis

Feng

Zhang

et al. 2021

Nat Commun

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“…Quantitative determination of NO in biological samples is particularly challenging given its short lifespan and low concentration in biological systems – generally estimated to be on the order of seconds and nanomolar respectively [10 , 11] . Several tools to detect NO in biological systems have been developed and reviewed [12] , [13] , [14] . Many of the commonly utilized methods suffer from some disadvantages [15 , 16] .…”

Section: Methods Detailsmentioning

confidence: 99%

“…Table 1 summarizes the major methods for detecting NO. The first method to be widely used for quantifying NO was the Griess test, in which NO 2 − , a natural autoxidation product of NO, is derivatized to a purple azo dye that can be quantified using spectrophotometry [13] . However, the NO autoxidation process is subject to interference in biological systems, as NO and its natural derivatives (e.g., NO 2 , N 2 O 3 , NO 2 − ) are readily trapped by biomolecules such as heme, glutathione, sulfhydryl, and unsaturated fatty acids [10 , 17 , 18] .…”

Section: Methods Detailsmentioning

confidence: 99%

Quantitative determination of nitric oxide from tissue samples using liquid chromatography—Mass spectrometry

2021

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“…Three major NOS isoforms are usually expressed by different cell types: the constitutive isoforms, i.e., endothelial NOS (e-NOS or NOS III) and neuronal NOS (nNOS or NOS I), and the inducible NOS (iNOS or NOS II) [ 1 ]. The isoforms got their names based on their functions or the type of tissues in which they were firstly found [ 6 ]. All isoforms use three cofactors: flavin adenine dinucleotide (FAD), flavin mononucleotide (FMN), and (6R)5,6,7,8-tetrahydro-L-biopterin (BH4).…”

Section: Nitric Oxidementioning

confidence: 99%

Nitric Oxide: From Gastric Motility to Gastric Dysmotility

Idrizaj

Traini

Vannucchi

et al. 2021

IJMS

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It is known that nitric oxide (NO) plays a key physiological role in the control of gastrointestinal (GI) motor phenomena. In this respect, NO is considered as the main non-adrenergic, non-cholinergic (NANC) inhibitory neurotransmitter responsible for smooth muscle relaxation. Moreover, many substances (including hormones) have been reported to modulate NO production leading to changes in motor responses, further underlying the importance of this molecule in the control of GI motility. An impaired NO production/release has indeed been reported to be implicated in some GI dysmotility. In this article we wanted to focus on the influence of NO on gastric motility by summarizing knowledge regarding its role in both physiological and pathological conditions. The main role of NO on regulating gastric smooth muscle motor responses, with particular reference to NO synthases expression and signaling pathways, is discussed. A deeper knowledge of nitrergic mechanisms is important for a better understanding of their involvement in gastric pathophysiological conditions of hypo- or hyper-motility states and for future therapeutic approaches. A possible role of substances which, by interfering with NO production, could prove useful in managing such motor disorders has been advanced.

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Nitric oxide detection methods in vitro and in vivo

Cited by 77 publications

References 113 publications

Facilitation of molecular motion to develop turn-on photoacoustic bioprobe for detecting nitric oxide in encephalitis

Facilitation of molecular motion to develop turn-on photoacoustic bioprobe for detecting nitric oxide in encephalitis

Quantitative determination of nitric oxide from tissue samples using liquid chromatography—Mass spectrometry

Nitric Oxide: From Gastric Motility to Gastric Dysmotility

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