Nitric oxide-dependent killing of<i>Candida albicans</i>by murine peritoneal cells during an experimental infection

Rementerı́a, Aitor; García-Tobalina, Roberto; Sevilla, Marı́a Jesús

doi:10.1111/j.1574-695x.1995.tb00112.x

Cited by 21 publications

(4 citation statements)

References 19 publications

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“…NO has been identified as a mediator in many physiological functions, including the regulation of vascular tone [2], cell-to-cell communication [3], and phagocytosis [4]. In order to correlate changes in the amount of NO with disease, it is important for human health to monitor the amount of NO.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of nitrate and nitrite in biological fluids by capillary electrophoresis and preliminary study on their determination by microchip capillary electrophoresis

Miyado¹,

Tanaka²,

Nagai³

et al. 2004

Journal of Chromatography A

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In order to develop a highly sensitive and high-throughput screening method for nitrogen monoxide metabolites in biological fluids, we have investigated the simultaneous determination of nitrite and nitrate, using capillary electrophoresis and microchip capillary electrophoresis. In capillary zone electrophoresis, a running buffer based on human serum components with high ionic strength has been developed for the determination of nitrite and nitrate in human serum and human saliva. We obtained successful separation of nitrite and nitrate in the serum and the saliva within 7 min under optimum analytical conditions. Linear calibration curves for nitrite and nitrate for both peak height and area were obtained by a standard addition method. The limits of detection obtained at a signal-to-noise ratio (S/N) of 3 for nitrite and nitrate in the serum were 2.6 and 1.5 microM, respectively. The values of the relative standard deviation of peak height for the serum with 9.2 microM nitrite and 20.9 microM nitrate were 5.7 and 4.1%, respectively. For on-site analysis with high-throughput screening, a microchip capillary electrophoresis method using a microchip made of quartz with a UV detector was developed. In this high-throughput format, using a running buffer with an electroosmotic flow modifier, the peaks of nitrite and nitrate in an artificial serum sample were obtained within 8 s. In high-resolution mode, using the buffer without electroosmotic flow modifier, the separation of nitrite and nitrate was obtained within 15 s. In high-resolution mode, using an artificial serum sample with 50 microM NO2- and 50 microM NO3-, the limits of detection (S/N = 3) of 41 microM for NO2- and 26 microM for NO3- were obtained. The method was applied to human serum and saliva. We obtained peaks due to nitrite and nitrate in 10-fold diluted saliva.

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Section: Introductionmentioning

confidence: 99%

Simultaneous determination of nitrate and nitrite in biological fluids by capillary electrophoresis and preliminary study on their determination by microchip capillary electrophoresis

Miyado¹,

Tanaka²,

Nagai³

et al. 2004

Journal of Chromatography A

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“…RNS has well characterised, highly candidacidal effects (Rementería, García-Tobalina and Sevilla, 1995;Vazquez-Torres et al, 1995;Tillmann, Gow and Brown, 2011;Navarathna, Lionakis and Roberts, 2019). Similarly, Macherla et al revealed NO-releasing nanoparticles inhibit the growth of C. albicans in vitro and in vivo (Macherla et al, 2012).…”

Section: Discussionmentioning

confidence: 97%

“…(Brown, 2011), supporting our conclusion that neutrophil suppression is an important strategy of C. albicans during infection that, if rescued, could represent an exciting therapeutic opportunity. RNS has well characterised, highly candidacidal effects (Rementería, García-Tobalina and Sevilla, 1995; Vazquez-Torres et al ., 1995; Tillmann, Gow and Brown, 2011; Navarathna, Lionakis and Roberts, 2019). Similarly, Macherla et al .…”

Section: Discussionmentioning

confidence: 99%

Candida albicanssuppression of neutrophil reactive nitrogen species is rescued by host Hif-1αin vivo

Burgess,

Hammond,

Szkuta

et al. 2023

Preprint

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Candidaspp. cause 750,000 cases per annum of invasive disease worldwide, with up to a 50% mortality rate. Poor efficacy of current antifungals, lack of vaccines and rising antifungal resistance rates point towards an urgent need to develop new therapies.Candida albicansis a human commensal fungus that can cause life-threatening invasive infection in immunocompromised individuals.C. albicansis able to manipulate host macrophages and neutrophils to escape phagosomal killing and has previously been shown to suppress reactive nitrogen species (RNS) productionin vitro. However, the effects ofC. albicanson RNSin vivoand the molecular and cellular mechanisms involved remain unclear. Using a zebrafish model, we aimed to characterise RNS suppression byC. albicans in vivo. We demonstrate thatC. albicanssuppressed neutrophil RNS both proximally and distally to the infection site in a partially active process, with heat-killedC. albicansnot reducing RNS to the same extent as live fungi. Using acar1Δ mutant, we show that fungal arginase is partially responsible for the reduction in neutrophil RNS. Stabilisation of Hif-1α, a transcription factor with a key role in immune regulation, rescued neutrophil RNS production duringC. albicansinfection, leading to improved infection outcomes. The protective effect of Hif-1α stabilisation was neutrophil- and nitric oxide synthase-dependent. Together, these data demonstrate that Hif-1α stabilisation can restore the neutrophil RNS response inC. albicansinfection, leading to improved infection outcomes, highlighting the potential of targeting Hif-1α and RNS in host directed therapies against fungal infections.

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“…NO · is identical to endothelium-derived relaxing factor [5,6] and is the principal signaling molecule in the relaxation of vascular smooth muscle cells [ 7± 9]. It has been implicated in other physiological processes such as neurotransmission [10], immunity [11] and inflammation [12]. Nitric oxide synthase (NOS) converts L-arginine and oxygen to citrulline and NO · [7].…”

Section: Introductionmentioning

confidence: 99%

Simultaneous analysis of nitrite, nitrate and the nicotinamide nucleotides by capillary electrophoresis: Application to biochemical studies and human extracellular fluids

et al. 1999

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A simple but rapid capillary electrophoresis method was developed for the measurement of nitrite and nitrate in human extracellular fluids and other aqueous solutions. The capabilities of the method were demonstrated by the measurement of endogenous nitrite and nitrate in plasma and serum samples from healthy volunteers, and serum and synovial fluid samples from rheumatoid arthritis patients. Furthermore, this method was used to simultaneously measure nicotinamide adenine dinucleotide, reduced (NADH), nicotinamide adenine dinucleotide (NAD+), nitrite, and nitrate, when studying the nitrite reductase activity of xanthine oxidase. The stability of nitrite was also investigated and it was found that when whole blood was spiked with nitrite and then processed, the nitrite was more stable in the plasma than in the serum. Our findings may help to explain the variations in basal nitrite concentrations reported in the literature.

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Nitric oxide-dependent killing ofCandida albicansby murine peritoneal cells during an experimental infection

Cited by 21 publications

References 19 publications

Simultaneous determination of nitrate and nitrite in biological fluids by capillary electrophoresis and preliminary study on their determination by microchip capillary electrophoresis

Simultaneous determination of nitrate and nitrite in biological fluids by capillary electrophoresis and preliminary study on their determination by microchip capillary electrophoresis

Candida albicanssuppression of neutrophil reactive nitrogen species is rescued by host Hif-1αin vivo

Simultaneous analysis of nitrite, nitrate and the nicotinamide nucleotides by capillary electrophoresis: Application to biochemical studies and human extracellular fluids

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