Nitric oxide concentrations in gas emanating from the tails of obese rats

Abstract

This study was undertaken to investigate the effects of oral L-arginine administration and exercising training on the NO concentration emanating from rat tail and NOx in plasma. Obese (fa/fa) Zucker rats (n = 22) were divided into four groups: (1) oral L-arginine administration (A) (n = 6), (2) exercise training (E), (3) exercise training + L-arginine administration (E + A) (n = 5), and (4) non-exercise training + non-L-arginine administration (N) (n = 6). The control (+/+) Zucker rats (n = 22) were also divided into the same four groups. The body weight of the E + A and the A groups was significantly lower than that of the N group. The NO concentration emitted from the tail was higher in the L-arginine (E + A and A) groups than in the non-L-arginine (E and N) groups in both obese and control rats. Exercise training did not affect the skin gas NO concentration in either obese or control rats. Plasma NOx concentrations in four obese rats were significantly higher than those observed in control rats. Exercise training did not influence the level of plasma NOx in obese or control rats. In conclusion, this study confirmed that L-arginine administration increases the skin gas NO concentration and obesity increases the plasma NOx level. The plasma NOx concentrations were not affected by L-arginine administration or exercise training in obese or control rats.

Application of Skin Gas GC/MS Analysis for Prediction of the Severity Scale of Parkinson’s Disease

“…The same group later reported that serum levels of caffeine and nine of its downstream metabolites were decreased, which was irrelevant to total caffeine intake or disease severity [9]. We also reported by mass spectrometry that the ratio of serum tyrosine to phenylalanine was less than 0.82 in 49% of PD patients, but in none of controls [10]. Trivedi et al recently reported that perillic aldehyde and eicosane were increased in sebum samples collected from the upper back in PD [11].…”

“…According to our personal experiences, breath gas is largely influenced by volatile substances produced or consumed by oral microbiota, whereas the skin gas is devoid of these factors. Although acquisition of the skin gas was more tedious than that of breath gas, we previously developed a method to reproducibly obtain gas samples from the skin [7][8][9][10].…”

Effect of different intensities of physical activity on cardiometabolic markers and vascular and cardiac function in adult rats fed with a high-fat high-carbohydrate diet

“… 69 On the other hand, no significant differences in serum nitric oxide concentrations were observed between groups, which is in agreement with a previous study that demonstrated no change in the level of plasma nitric oxide after 4 weeks of moderate intensity exercise in Zucker obese rats. 103 Results of this study showed that sedentary behavior, LIT, and HIIT had no effect on inflammatory and oxidative stress markers. However, because this is the first study to examine the influence of an HFHC diet with different intensities of PA on markers of inflammation and oxidative stress, more research is required to fully understand these results.…”