Nitric oxide as an active substance in the enteric neurons of the porcine digestive tract in physiological conditions and under intoxication with bisphenol A (BPA)

Szymańska, Kamila; Całka, Jarosław; Gonkowski, Sławomir

doi:10.1016/j.niox.2018.08.001

Cited by 23 publications

(39 citation statements)

References 87 publications

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“…In this study, we have shown that nNOS occurs in relatively high proportion of enteric neurons, which is in agreement with previous studies, where nitrergic structures have been noted in different mammalian species such as pigs [20,38], guinea pigs [39], mice [39,40] rats [41,42] and humans [43]. The current findings are congruent with the available literature, where more numerous NOS-positive neurons were found in the myenteric plexus [22,44].…”

Section: Discussionsupporting

confidence: 93%

“…The present immunohistochemical studies revealed that in the jejunum of pigs treated with high doses of acetylsalicylic acid subpopulations of NOS-, VIP-and CART-IR myenteric and submucous neurons were statistically altered. Previous studies have also shown changes in these neuroactive substances in the swine gastrointestinal tract during induced inflammatory processes [3,20,24]. It is believed that the capacity to change neuropeptide/neurotransmitter content, termed enteric neuroplasticity, is an adaptation to an unfavorable enteric microenvironment [29].…”

Section: Discussionmentioning

confidence: 98%

“…Adaptation to the changing environmental conditions includes changing in the chemical coding of neurons by enhanced expression of some neurotransmitters and neuromodulators and decreased of others [2,18,19]. Vasoactive intestinal peptide (VIP) and nitric oxide synthase (NOS) are well-known and widely described biologically active substances found in the ENS [2,3,20,21]. Furthermore, these neurotransmitters are involved in gut neuronal defense system [22].…”

Section: Introductionmentioning

confidence: 99%

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Neurochemical Plasticity of nNOS-, VIP- and CART-Immunoreactive Neurons Following Prolonged Acetylsalicylic Acid Supplementation in the Porcine Jejunum

Rząp

Czajkowska

Całka

2020

IJMS

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Aspirin, also known as acetylsalicylic acid (ASA), is a commonly used anti-inflammatory drug that has analgesic and antipyretic properties. The side effects are well known, however, knowledge concerning its influence on gastric and intestinal innervation is limited. The enteric nervous system (ENS) innervates the whole gastrointestinal tract (GIT) and is comprised of more than one hundred million neurons. The capacity of neurons to adapt to microenvironmental influences, termed as an enteric neuronal plasticity, is an essential adaptive response to various pathological stimuli. Therefore, the goal of the present study was to determine the influence of prolonged ASA supplementation on the immunolocalization of neuronal nitric oxide synthase (nNOS), vasoactive intestinal peptide (VIP) and cocaine- and amphetamine- regulated transcript peptide (CART) in the porcine jejunum. The experiment was performed on 8 Pietrain × Duroc immature gilts. Using routine double-labelling immunofluorescence, we revealed that the ENS nerve cells underwent adaptive changes in response to the induced inflammation, which was manifested by upregulated or downregulated expression of the studied neurotransmitters. Our results suggest the participation of nNOS, VIP and CART in the development of inflammation and may form the basis for further neuro-gastroenterological research.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

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Neurochemical Plasticity of nNOS-, VIP- and CART-Immunoreactive Neurons Following Prolonged Acetylsalicylic Acid Supplementation in the Porcine Jejunum

Rząp

Czajkowska

Całka

2020

IJMS

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“…Previous studies have shown a link between excessive exposure to BPA and various civilization diseases, including diabetes, hypertension, heart attack, or neoplasms [ 31 ]. Moreover, it is known that BPA changes the neurochemical characterization of neurons and nerves within the peripheral nervous system in various internal organ including intestine, heart, and liver [ 32 , 33 , 34 , 35 ], but until now the participation of NRG-1 in mechanisms connected with BPA intoxication has not been studied.…”

Section: Introductionmentioning

confidence: 99%

The Influence of Bisphenol A (BPA) on Neuregulin 1-Like Immunoreactive Nerve Fibers in the Wall of Porcine Uterus

Rytel

2018

IJMS

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Bisphenol A (BPA), a substance commonly used in the manufacture of plastics, shows multidirectional negative effects on humans and animals. Due to similarities to estrogens, BPA initially leads to disorders in the reproductive system. On the other hand, it is known that neuregulin 1 (NRG-1) is an active substance which enhances the survivability of cells, inhibits apoptosis, and protects tissues against damaging factors. Because the influence of BPA on the nervous system has also been described, the aim of the present study was to investigate for the first time the influence of various doses of BPA on neuregulin 1-like immunoreactive (NRG-1-LI) nerves located in the porcine uterus using the routine single- and double-immunofluorescence technique. The obtained results have shown that BPA increases the number and affects the neurochemical characterization of NRG-1-LI in the uterus, and changes are visible even under the impact of small doses of this toxin. The character of observed changes depended on the dose of BPA and the part of the uterus studied. These observations suggest that NRG-1 in nerves supplying the uterus may play roles in adaptive and protective mechanisms under the impact of BPA.

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“…Nitric oxide is a gaseous neurotransmitter and/or neuromodulator, which is synthetized from Larginine with a reaction which nitric oxide synthases (nNOS) play a role [6,7]. NO is an essential mediator in the relaxation of the vascular smooth muscles in the gastrointestinal tract, respiratory system and cavernous tissues [8]. NO has different biological effects at physiological concentrations in almost all organ systems.…”

Section: Introductionmentioning

confidence: 99%

Neurotoxic effects of bisphenol A on SH-SY5Y neuroblastoma cells via nitric oxide

Ayazgök¹,

Küçükkılınç²

2019

jrp

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Bisphenol A (BPA, 2,2-bis(4-hydroxyphenyl)propane)polycarbonate is an industrial component frequently used as an additive in the construction of epoxy resin and other non-polymer plastics. In this study cytotoxicity of low dose BPA (0.01 μM-100 μM) and NO levels in SH-SY5Y cells were assessed together to determine whether there is a correlation between of them. MTT Cytotoxicity assays were performed by applying BPA at increasing doses (0.01 μM-100 μM) for 8, 24, 72 hours. Measurements to determine the nitric oxide (NO) levels of the cells, were made using the Griess method at the same time intervals and at the equal doses with BPA. It is found that percentage viability values in SH-SY5Y cells treated with 100 µM BPA for 8 hours, is reduced statistically significant compared to control groups (p<0.001). A significant correlation was found between cytotoxicity results and NO levels of cells treated BPA (0.01 μM-100 μM) for 8 and 72 hours (p<0.01, p<0.001 respectively). Neurotoxic effects of low dose BPA exposure on SH-SY5Y cells may be explained with increase in the levels of NO as a result from different parameters. One of these parameters could be a disorder in the synthesis of nNOS on different stages such as transcription and translation.

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Nitric oxide as an active substance in the enteric neurons of the porcine digestive tract in physiological conditions and under intoxication with bisphenol A (BPA)

Cited by 23 publications

References 87 publications

Neurochemical Plasticity of nNOS-, VIP- and CART-Immunoreactive Neurons Following Prolonged Acetylsalicylic Acid Supplementation in the Porcine Jejunum

Neurochemical Plasticity of nNOS-, VIP- and CART-Immunoreactive Neurons Following Prolonged Acetylsalicylic Acid Supplementation in the Porcine Jejunum

The Influence of Bisphenol A (BPA) on Neuregulin 1-Like Immunoreactive Nerve Fibers in the Wall of Porcine Uterus

Neurotoxic effects of bisphenol A on SH-SY5Y neuroblastoma cells via nitric oxide

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