Nitric oxide as a bioactive molecule in the regulation of chalcone synthase during jasmonic acid mediated defense signaling in ginger

Aiswarya, G.; Devakumar, Lovely Jael Paul Solomon; Vijayanathan, Mallika; Soniya, E. V.

doi:10.1007/s11240-016-1129-8

Cited by 6 publications

(2 citation statements)

References 16 publications

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“…In the qRT-PCR research on ginger, common reference genes (TUB, actin, EF1α, etc.) have been used (Girija et al, 2017;Jiang et al, 2017;Chen et al, 2020), but this study found that these are not the best choice, and some common reference genes even rank lower in this study. Therefore, the selection of appropriate reference genes in ginger is very important for the standardization of target genes.…”

Section: Discussionmentioning

confidence: 83%

Identification of Ginger (Zingiber officinale Roscoe) Reference Genes for Gene Expression Analysis

Liu

et al. 2020

Front. Genet.

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Quantitative real-time PCR (qRT-PCR) is widely used in the detection of gene expression level. However, there is no suitable ginger reference gene for qPCR analysis. Therefore, it is the primary task to select and validate the appropriate ginger reference gene to normalize the expression of target genes. In this study, 14 candidate reference genes were selected and analyzed in different tissues (leaf, and rhizome), different development stages, different varieties, and abiotic stress (ABA and salt stress). Expression stability was calculated using geNorm and NormFinder, Bestkeeper, and RefFinder. For abiotic stress and total conditions, 28S and COX were identified as the most stable genes. In addition, RPII was the most stable in the different development stages and different varieties. TEF2 and RPL2 were the least stably expressed in the tissue and all the conditions. In order to verify the feasibility of these genes as reference genes, we used the most stable and least stable reference genes to normalize the expression levels of ZoSPS genes under different conditions. This work can provide theoretical support for future research on ginger gene expression.

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Section: Discussionmentioning

confidence: 83%

Identification of Ginger (Zingiber officinale Roscoe) Reference Genes for Gene Expression Analysis

Liu

et al. 2020

Front. Genet.

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“…Nitric oxide (NO) plays diverse regulatory roles in plant growth and development. It promotes the lateral bud germination of explants, induces callus formation, and delays callus stress, among other functions [15,16]. Notably, NO exhibits antioxidant activity within plant cells.…”

Section: Introductionmentioning

confidence: 99%

Effect of Nitric Oxide on Browning of Stem Tip Explants of Malus sieversii

Yang,

Liu,

Qin

et al. 2023

Horticulturae

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Browning is a major problem in the tissue culture of woody plants. Previous studies have shown that nitric oxide (NO) plays a role in regulating plant responses to stress, but its effect on browning in the tissue culture of Malus remains unclear. This study aimed to investigate the impact of exogenous NO donor sodium nitroprusside (SNP) on the browning of Malus sieversii stem tip explants. The results demonstrated that the addition of 50 μM SNP significantly reduced explant browning. Further analysis revealed that exogenous NO decreased the browning index (BD) and levels of malondialdehyde (MDA), hydrogen peroxide (H2O2), and superoxide anion radical O2−. Additionally, NO treatment increased the activity of antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX). NO treatment also enhanced the activity of phenylalanine ammonia lyase (PAL), which contributed to the accumulation of flavonoids and inhibited the activity of polyphenol oxidase (PPO) and peroxidase (POD), which are key enzymes involved in the browning process. Furthermore, 50 μM SNP significantly promoted the accumulation of non-enzymatic antioxidants such as proline (Pro), soluble sugar (SS), and soluble protein (SP). Therefore, the results suggest that NO is able to counteract excessive reactive oxygen species (ROS) damage by enhancing both the enzymatic and non-enzymatic antioxidant systems, resulting in a reduction in browning in stem tip explants. Consequently, an improvement in the in vitro propagation efficiency of Malus sieversii shoot tip explants can be achieved.

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Exogenous Nitric Oxide‐ and Hydrogen Sulfide‐induced Abiotic Stress Tolerance in Plants

Hasanuzzaman

Bhuyan

Mohsin

et al. 2020

Protective Chemical Agents in the Amelioration of Plant Abiotic Stress

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Nitric oxide as a bioactive molecule in the regulation of chalcone synthase during jasmonic acid mediated defense signaling in ginger

Cited by 6 publications

References 16 publications

Identification of Ginger (Zingiber officinale Roscoe) Reference Genes for Gene Expression Analysis

Identification of Ginger (Zingiber officinale Roscoe) Reference Genes for Gene Expression Analysis

Effect of Nitric Oxide on Browning of Stem Tip Explants of Malus sieversii

Exogenous Nitric Oxide‐ and Hydrogen Sulfide‐induced Abiotic Stress Tolerance in Plants

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